“冷泡茶”是一种新型茶类,为确保产品的安全性,对“冷泡茶”进行安全性评价研究很有必要。研究结果表明：受试物茶样采用最大耐受剂量法（40βml/kg·BW）经口染毒受试小鼠,测得小鼠经口急性毒性试验LD₅₀>40βml/kg·BW,换算为茶叶量为LD₅₀>20βg/kg·BW,可以判定为无毒;基于LD₅₀测定结果,受试物茶样设一次最大灌胃量（20βml/kg·BW）为高剂量组,并设中、低剂量组,试验结果表明小鼠骨髓细胞微核试验为阴性,即在所测试水平,茶样对昆明种小鼠没有精子致畸作用和致微核作用。说明该产品具有较高的食用安全性。

“Cold-making tea” is a new type of tea. To ensure the safety of cold-brewing tea, it is necessary to conduct the research on its property and safety. Results showed that the mice were ingested the tea sample by means of maximal tolerant dosage(40βml/kg·BW)method, The median acute oral lethal dose(LD₅₀)>40βml/kg·BW and it equals to LD₅₀>20βg/kg·BW of the dried tea sample. It can be classified as the class of nontoxic . Based on the above results, three-treatment levels（the maximum dosage 20βml/kg·BW）of the tea infusion were ingested into the mice stomach. It was showed that the result of mice micronucleual test of bone marrow cell was negitive. which means the tea sample was no teratogenicity on sperm and had no micronucleus effect on Kunming mice under the test levels. Results showed that the tea sample was nontoxic and safe.