采用MTT方法研究表没食子儿茶素没食子酸酯（EGCG）对人结肠癌细胞HT-29、人乳腺癌细胞MCF-7和人宫颈癌细胞Hela生长的影响。通过检测培养基中乳酸脱氢酶的活力研究EGCG对三种细胞的细胞毒作用,用流式细胞仪检测EGCG对HT-29的凋亡影响,用HPLC方法分析EGCG在培养基DMEM/F-12中的稳定性。以上研究均设加入SOD和不加SOD两个对照组。研究表明：与没有SOD存在条件下相比,加入SOD后,细胞存活率下降,培养基中LDH活性增强,HT-29细胞凋亡率上升;EGCG在培养基中的稳定性增强。
Effects of epigallocatechin-3-gallate (EGCG) on the growth of human colonic cancer cells HT-29, human breast cancer MCF-7 and human cervical cancer Hela were investigated by MTT assay. Cytotoxicity of EGCG on these cells was detected by determining the activity of LDH in culture medium. Induction of apoptosis in HT-29 cell after the EGCG treatment was analyzed by flow cytometry. Stability of EGCG in DMEM/F-12 was determined by HPLC and all studies above were compared in the presence or absence of SOD. Investigation showed that cells survival percentage deceased and activity of LDH in the culture medium increased after adding SOD. The apoptotic rate of HT-29 and the stability of EGCG in culture increased in the presence of SOD comparing to absence of SOD.
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