SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响

doi:10.13305/j.cnki.jts.2007.04.006

茶叶科学 ›› 2007, Vol. 27 ›› Issue (4): 293-298.doi: 10.13305/j.cnki.jts.2007.04.006

SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响

张斌, 夏艳秋, 金礼吉, 徐永平

大连理工大学环境与生命学院生物科学与工程系,辽宁大连 116024

收稿日期:2007-03-19 修回日期:2007-05-31 出版日期:2007-12-25 发布日期:2019-09-11
作者简介:张斌（1981— ）,男,辽宁本溪市人,硕士研究生,主要从事茶叶活性物质抗肿瘤作用的研究。
基金资助:
国家杰出青年基金（30125034）

Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate

ZHANG Bin, XIA Yan-qiu, JIN Li-ji, XU Yong-ping

Department of Bioscience and Biotechnology, School of of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China

Received:2007-03-19 Revised:2007-05-31 Online:2007-12-25 Published:2019-09-11

摘要/Abstract

摘要： 采用MTT方法研究表没食子儿茶素没食子酸酯（EGCG）对人结肠癌细胞HT-29、人乳腺癌细胞MCF-7和人宫颈癌细胞Hela生长的影响。通过检测培养基中乳酸脱氢酶的活力研究EGCG对三种细胞的细胞毒作用,用流式细胞仪检测EGCG对HT-29的凋亡影响,用HPLC方法分析EGCG在培养基DMEM/F-12中的稳定性。以上研究均设加入SOD和不加SOD两个对照组。研究表明：与没有SOD存在条件下相比,加入SOD后,细胞存活率下降,培养基中LDH活性增强,HT-29细胞凋亡率上升;EGCG在培养基中的稳定性增强。

关键词: EGCG, SOD, HT-29细胞

Abstract: Effects of epigallocatechin-3-gallate (EGCG) on the growth of human colonic cancer cells HT-29, human breast cancer MCF-7 and human cervical cancer Hela were investigated by MTT assay. Cytotoxicity of EGCG on these cells was detected by determining the activity of LDH in culture medium. Induction of apoptosis in HT-29 cell after the EGCG treatment was analyzed by flow cytometry. Stability of EGCG in DMEM/F-12 was determined by HPLC and all studies above were compared in the presence or absence of SOD. Investigation showed that cells survival percentage deceased and activity of LDH in the culture medium increased after adding SOD. The apoptotic rate of HT-29 and the stability of EGCG in culture increased in the presence of SOD comparing to absence of SOD.

Key words: EGCG, SOD, HT-29 cells

中图分类号:

S571.1
R983

张斌, 夏艳秋, 金礼吉, 徐永平. SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响[J]. 茶叶科学, 2007, 27(4): 293-298. doi: 10.13305/j.cnki.jts.2007.04.006.

ZHANG Bin, XIA Yan-qiu, JIN Li-ji, XU Yong-ping. Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate[J]. Journal of Tea Science, 2007, 27(4): 293-298. doi: 10.13305/j.cnki.jts.2007.04.006.

参考文献

[1] Chi C, Guoxiang S, Vidya H, et al. Epigallocatechin- 3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells[J]. Carcinogenesis, 2003, 24(8): 1369~1378.
[2] Masatoshi Yokoyama, Mitsuyo Noguchi, Yoshifumi Nakao, et al. The tea polyphenol, (-)-epigallocatechin gallate effects on growth, apoptosis, and telomerase activity in cervical cell lines[J]. Gynecologic Oncology, 2004, 92(1): 197~204.
[3] Mittal A, Pate MS, Wylie RC, et al. EGCG down-regulates telomerase in human breast carcinoma MCF-7 cells, leading to suppression of cell viability and induction of apoptosis[J]. Int J Oncol., 2004, 24(3): 703~710.
[4] 陈勋, 于海宁, 沈生荣. EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响[J]. 茶叶科学, 2006, 26(3): 219~224.
[5] Mochizuki M, Yamazaki S, Kano K, et al. Kinetic analysis and mechanistic aspects of autoxidation of catechins[J]. Biochim Biophys Acta, 2002, 1569(1~3): 35~44.
[6] Nakayama T, Enoki Y, Hashimoto K.Hydrogen peroxide formation during catechin oxidation is inhibited by superoxide dismutase[J]. Food Sci Technol Int, 1995(1): 65~69.
[7] Miura YH, Tomita I, Watanabe T, et al. Fukui S. Active oxygens generation by flavonoids[J]. Biol Pharm Bull, 1998, 21(2): 93~96.
[8] Yang CS, Maliakal P, Meng X.Inhibition of carcinogenesis by tea[J]. Annu ReV Pharmacol Toxicol, 2002(42): 25~54.
[9] Hong J, Lu H, Meng X, et al. Stability, cellular uptake, biotransformation, and efflux of tea polyphenol (-)- epigallocatechin-3-gallate in HT-29 human colon adenocarcinoma cells[J]. Cancer Res, 2002, 62: 7241~7246.
[10] Zhe H, Shengmin S, Hui Y, et al. Mechanism of Action of (-)-Epigallocatechin-3-Gallate: Auto-oxidation-Dependent Inactivation of Epidermal Growth Factor Receptor and Direct Effects on Growth Inhibition in Human Esophageal Cancer KYSE 150 Cells[J]. Cancer Res, 2005, 65(17): 8049~8056.

[1]	陈珂, 王苑竹, 杨晓颖, 张冬英, 朱强强. 壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响[J]. 茶叶科学, 2022, 42(5): 731-739.
[2]	俞蓉欣, 郑芹芹, 陈红平, 张劲松, 张相春. 儿茶素生物医用纳米材料研究进展[J]. 茶叶科学, 2022, 42(4): 447-462.
[3]	张旖旎, 吉铮. 表没食子儿茶素没食子酸酯（EGCG）研究的文献计量分析[J]. 茶叶科学, 2022, 42(3): 423-434.
[4]	张建勇, 陈琳, 崔宏春, 王伟伟, 薛金金, 熊春华, 江和源. PBD和RSM联用优化聚酯型儿茶素A化学合成技术参数[J]. 茶叶科学, 2020, 40(1): 51-62.
[5]	孙丽丽, 曾祥泉, NileshWGaikwad, 王欢, 须海荣, 叶俭慧. 绿茶儿茶素类生物标记物的检测及应用[J]. 茶叶科学, 2017, 37(5): 429-441.
[6]	黄翔翔, 杨哲, 禹利君. 绿茶、EGCG预防及缓解香烟烟雾诱导COPD的研究进展[J]. 茶叶科学, 2017, 37(4): 332-338.
[7]	祁洁, 徐颖磊, 梁文怡, 费朵, 乌昕儿, 金建昌, 杜琪珍, 许勇泉, 高颖. EGCG纳米载体制备技术及其对EGCG活性影响的研究进展[J]. 茶叶科学, 2017, 37(2): 119-129.
[8]	张静, 黄建安, 蔡淑娴, 易晓芹, 刘建军, 王英姿, 田丽丽, 刘仲华. 茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤[J]. 茶叶科学, 2016, 36(6): 655-662.
[9]	柳敏, 饶国武, 华允芬. EGCG衍生物合成及药理活性研究进展[J]. 茶叶科学, 2016, 36(2): 119-130.
[10]	叶晓锋, 张芳, 朱军莉, 张蕾, 谢杜梅. EGCG抑制波罗的海希瓦氏菌生物被膜和腐败活性的研究[J]. 茶叶科学, 2016, 36(2): 201-209.
[11]	孟庆, 屠幼英. TF₁与EGCG在Caco-2细胞中的吸收规律研究[J]. 茶叶科学, 2015, 35(3): 233-238.
[12]	高媛圆, 毛立民, 徐平, 王岳飞. 茶多酚防治2型糖尿病的分子机理研究进展[J]. 茶叶科学, 2015, 35(3): 239-247.
[13]	陈怡君, 熊立瑰, 黄建安, 龚雨顺, 刘仲华. EGCG对细胞的促氧化作用研究进展[J]. 茶叶科学, 2015, 35(2): 130-136.
[14]	高静, 禹利君, 李晓焕, 高鹏, 周清明. EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究[J]. 茶叶科学, 2015, 35(2): 171-178.
[15]	刘晓慧, 揭国良, 林康, 吴媛媛, 王岳飞, 屠幼英. EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究[J]. 茶叶科学, 2014, 34(3): 239-247.

SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响

Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10