壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响

doi:10.13305/j.cnki.jts.2022.05.003

茶叶科学 ›› 2022, Vol. 42 ›› Issue (5): 731-739.doi: 10.13305/j.cnki.jts.2022.05.003

壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响

陈珂^1,2, 王苑竹^1,2, 杨晓颖^1,3, 张冬英^1,3, 朱强强^1,2,*

1.云南农业大学普洱茶学教育部重点实验室,云南昆明 650201;
2.云南农业大学食品科学技术学院,云南昆明 650201;
3.云南农业大学理学院,云南昆明 650201

收稿日期:2022-04-11 修回日期:2022-06-20 出版日期:2022-10-15 发布日期:2022-10-28
通讯作者: * tianjiao125@126.com
作者简介:陈珂,女,硕士研究生,主要从事食品成分与代谢免疫研究,877525918@qq.com。
基金资助:
云南省基础研究专项-青年项目（202101AU070084）、云南农业大学科研启动基金（KY2019-24）、国家自然科学基金（31760226）

Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice

CHEN Ke^1,2, WANG Yuanzhu^1,2, YANG Xiaoying^1,3, ZHANG Dongying^1,3, ZHU Qiangqiang^1,2,*

1. Key Laboratory of Pu-erh Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China;
2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
3. College of Science, Yunnan Agricultural University, Kunming 650201, China

Received:2022-04-11 Revised:2022-06-20 Online:2022-10-15 Published:2022-10-28

摘要/Abstract

摘要： 表没食子儿茶素没食子酸酯（EGCG）作为茶叶中主要生物活性成分,具有良好的生理功能,但低稳定性使其容易被氧化降解,生物利用率低。利用羧甲基壳聚糖（CMC）、壳聚糖盐酸盐（CHC）、β-乳球蛋白（β-LG）作为壁材,制备壳聚糖复合β-乳球蛋白负载EGCG纳米粒,通过透射电镜、结构表征（粒径、Zeta电位测定）对颗粒微观形态进行观察,利用高效液相色谱仪对颗粒包埋率、模拟胃肠液释放率进行测定,最后建立糖尿病小鼠模型,探究包埋后颗粒的降血糖活性。结果表明,CS-β-LG-EGCG纳米粒结构完整、粒径10~100 nm、粒子分散;包埋率大于50%,且在肠液和胃液中具有缓释作用;CS-β-LG-EGCG纳米粒与胰岛素无拮抗作用,与未包埋的EGCG相比,包埋后颗粒具备的缓释作用可减缓血糖的回升。

关键词: EGCG, 壳聚糖, β-乳球蛋白, 模拟胃液, 糖尿病

Abstract: As the main bioactive component in tea, EGCG has abundant physiological functions. However, its low stability makes it easy to be degraded and has low bioavailability. In this experiment, carboxymethyl chitosan (CMC), chitosan hydrochloride (CHC), and β-lactoglobulin (β-LG) were used as wall materials to encapsulate EGCG. The microscopic morphology of particles were observed through electron microscope microscopic morphological observation, structural characterization (measurement of particle size, Zeta potential). The entrapment efficiency and simulated gastrointestinal fluid release rate were determined by HPLC. Finally, the activities of nanoparticles were explored by measuring the effect of nanoparticles on blood glucose in diabetic mice. The result shows that the prepared CS-β-LG-EGCG nanoparticles had complete structures, particle size of 10-100 nm, and certain dispersibilities. The entrapment efficiency was greater than 50%, and it had a slow-release effect in intestinal and gastric juices. CS-β-LG-EGCG nanoparticles had no antagonistic effect with insulin. Compared with uncoated EGCG, the sustained-release effect of the particles can slow down the recovery of blood glucose.

Key words: EGCG, chitosan, β-lactoglobulin, simulated gastric juice, diabetes

中图分类号:

陈珂, 王苑竹, 杨晓颖, 张冬英, 朱强强. 壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响[J]. 茶叶科学, 2022, 42(5): 731-739. doi: 10.13305/j.cnki.jts.2022.05.003.

CHEN Ke, WANG Yuanzhu, YANG Xiaoying, ZHANG Dongying, ZHU Qiangqiang. Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice[J]. Journal of Tea Science, 2022, 42(5): 731-739. doi: 10.13305/j.cnki.jts.2022.05.003.

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壳聚糖复合β-乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响

Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice

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