对不同外植体来源的茶树培养细胞的茶氨酸生物合成能力、适宜培养细胞合成茶氨酸的培养基条件、培养细胞茶氨酸生物合成动态和更新培养基对提高培养细胞茶氨酸累积含量的效果等进行了分析。在一个培养周期中,培养细胞的茶氨酸累积高峰出现在第11βd左右。在以每10βd更新一次培养基的条件下,培养细胞茶氨酸合成能力可维持至第30βd左右,达到细胞干重的近20%。
The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30th day and reached a high peak of 20% cell dry weight.
[1] 吕毅,郭雯飞,倪捷儿,等. 茶氨酸的生理作用及合成[J]. 茶叶科学. 2003,23(1):1-5.
[2] 钟俊辉,陶文沂. 茶愈伤组织培养及茶氨酸的积累[J]. 无锡轻工大学学报. 1997,16:1-7.
[3] 陈瑛,陶文沂. 几种激素对茶愈伤组织合成茶氨酸的影响[J]. 无锡轻工大学学报. 1998,17:74-77.
[4] Takanobu Matsuura, Takami Kakuda, Tatsuyaki Kinoshita, et al. Theanine formation by Tea Suspension Cells. Biosci.Biotech[J]. Biochem, 1992, 56(8):1175-1181.
[5] T. Matsuura, T. Kakuda.Effcts of Precursor,Temperature and Illuminatin on Theanine Accumulation in Tea Callus[J]. Agric Biol Chem, 1990, 54(9): 2283-2286.
[6] Tsutomu Furuya, Yutaka Orihara, Yumiko Tsuda.Caffeine and Theanine From Cultured Cells of Camellia Sinensis[J]. Phytochemistry. 1990, 29(8):2539-2543.
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