茶树悬浮细胞茶氨酸生物合成动态研究

成浩; 高秀清

doi:10.13305/j.cnki.jts.2004.02.008

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茶叶科学 ›› 2004, Vol. 24 ›› Issue (2) : 115-118. DOI: 10.13305/j.cnki.jts.2004.02.008

茶树悬浮细胞茶氨酸生物合成动态研究

成浩, 高秀清

作者信息 +

Dynamics of Theanine Biosynthesis in Tea Suspension Cells

CHENG Hao, GAO Xiu-qing

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摘要

对不同外植体来源的茶树培养细胞的茶氨酸生物合成能力、适宜培养细胞合成茶氨酸的培养基条件、培养细胞茶氨酸生物合成动态和更新培养基对提高培养细胞茶氨酸累积含量的效果等进行了分析。在一个培养周期中,培养细胞的茶氨酸累积高峰出现在第11βd左右。在以每10βd更新一次培养基的条件下,培养细胞茶氨酸合成能力可维持至第30βd左右,达到细胞干重的近20%。

Abstract

The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11^th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30^th day and reached a high peak of 20% cell dry weight.

导出引用

成浩, 高秀清. 茶树悬浮细胞茶氨酸生物合成动态研究[J]. 茶叶科学. 2004, 24(2): 115-118 https://doi.org/10.13305/j.cnki.jts.2004.02.008

CHENG Hao, GAO Xiu-qing. Dynamics of Theanine Biosynthesis in Tea Suspension Cells[J]. Journal of Tea Science. 2004, 24(2): 115-118 https://doi.org/10.13305/j.cnki.jts.2004.02.008

中图分类号： Q946

参考文献

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