茶氨酸的衍生化及毛细管电泳定量技术

doi:10.13305/j.cnki.jts.2004.02.009

茶叶科学 ›› 2004, Vol. 24 ›› Issue (2): 119-123.doi: 10.13305/j.cnki.jts.2004.02.009

茶氨酸的衍生化及毛细管电泳定量技术

李平¹, 宛晓春^{1, *}, 李健¹, 张正竹¹, 沈佐君²

1. 安徽农业大学轻工业学院,农业部茶叶生物化学与生物技术重点开放实验室,安徽合肥 230036;
2. 安徽省省立医院临床检测中心, 安徽合肥 230001

收稿日期:2003-10-24 出版日期:2004-06-25 发布日期:2019-09-16
基金资助:
国家自然基金（30270853）及安徽省教育厅自然基金（2002kj085）资助课题

Derivation of Theanine and Its Determination by Capillary Electrophoresis

LI Ping¹, WAN Xiao-chun¹, LI Jian¹, ZHANG Zheng-zhu¹, SHEN Zuo-jun²

1. Anhui Agricultural University, Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Agriculture, Hefei 230036, China;
2. Anhui Provincial Hospital, Anhui Provincial Center for Clinical Laboratory, Hefei 230001, China

Received:2003-10-24 Online:2004-06-25 Published:2019-09-16

摘要/Abstract

摘要： 采用胶束电动力学毛细管电泳技术定量茶氨酸,以2,4-二硝基氟苯为衍生化试剂,建立茶氨酸定量的新方法。运行缓冲液为pH9.8的0.03βmol/L四硼酸钠缓冲液体系,分离电压为28kV,分离温度为17℃,在360βnm下进行检测。研究了不同pH、反应时间、反应温度、缓冲液浓度对衍生化反应的影响,确立了衍生化反应的最佳条件。证实了4℃和25℃环境下衍生化产物可以稳定保存5天。测定了茶氨酸衍生化方法的性能指标：线性范围为0.2~5βmmol/L（r²=0.993）,最低检测限为0.05βmmol/L。

关键词: 茶学, 茶氨酸, 衍生化, 毛细管电泳, 定量

Abstract: A novel derivative method for determination of theanine was developed by micellar electrokinetic capillary chromatography (MECC). Samples were derivated with 2,4-dinitrofluorobenzen. The buffer solution used in this method was 0.03mol/L sodium borate solution (pH9.8). Column temperature was 17℃. The separating voltage was 28βkV and detection at 360βnm. Factors of pH value, reaction time, reaction temperature and concentration of buffer were optimized. The products of derivation of theanine can be stable for five days at 4℃ or 25℃. The linearity of this assay was 0.2~5βmmol/L (r²=0.993). The minimal detection limit (S/N=2) was 0.05βmmol/L.

Key words: Tea science, theanine, derivative, capillary electrophoresis, determination

中图分类号:

Q946

李平, 宛晓春, 李健, 张正竹, 沈佐君. 茶氨酸的衍生化及毛细管电泳定量技术[J]. 茶叶科学, 2004, 24(2): 119-123. doi: 10.13305/j.cnki.jts.2004.02.009.

LI Ping, WAN Xiao-chun, LI Jian, ZHANG Zheng-zhu, SHEN Zuo-jun. Derivation of Theanine and Its Determination by Capillary Electrophoresis[J]. Journal of Tea Science, 2004, 24(2): 119-123. doi: 10.13305/j.cnki.jts.2004.02.009.

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茶氨酸的衍生化及毛细管电泳定量技术

Derivation of Theanine and Its Determination by Capillary Electrophoresis

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