用热水浸泡法从粗老茶叶中提取茶叶多糖,在碱性条件下,茶叶多糖水溶液与三氯化铁反应合成茶叶多糖铁配合物(TPC),并用邻菲罗啉分光光度法和原子吸收光谱法测定TPC中铁(Ⅲ)的含量。实验结果表明:TPC是深棕红色无定型粉末, 易溶于水,在pH值3~12范围内不沉淀,不水解。TPC中铁(Ⅲ)的含量:邻菲罗啉分光光度法测定结果为20.71%,原子吸收光谱法测定结果为20.13%。
Tea polysaccharide was abstracted with warm water, tea polysaccharide-Fe(Ⅲ)complex(TPC) was synthesized with tea polysaccharide and FeCl3 under the alkaline condition, The determination of Fe(Ⅲ) was conducted with the method of ferroin-Fe(Ⅱ) and atomic absorption spectrophometry. The results showed that: TPC is heavy brownish red and shapeless powder. It can dissolve in water, its solution is very stable at the pH from 3 to 12. Content of Fe(Ⅲ)in TPC is 20.71% by the method of ferroin- Fe(Ⅱ)and 20.13% by the method of atomic absorption.
[1] 马慧民, 曹庆弘. 多糖铁胶囊市场推广策划分析[J]. 中国药房, 2003. 14(10): 584~586.
[2] Bannister WH, Bannister JV, Searle AJ, et al. The reaction of superoxide radicals with metal picolinate complexes[J]. Inorg Chem Acta , 1983, 78: 139~142.
[3] Sas G, Nemesaszky E, Brauer H.On the therapcutic effects of trivalent and divalent iron deficiency anemia[J]. Arznelm-Forsch/Drug Res, 1984, 34(1): 1575~1579.
[4] 汪东风, 李俊, 王常红, 等. 茶叶多糖的组成及免疫活性研究[J]. 茶叶科学, 2000, 20(1): 45~50.
[5] 叶盛, 程玉祥, 汪东风. 茶叶多糖及其生物活性[J]. 茶叶科学技术, 2000, 1: 1~4.
[6] 罗明志, 严明潮. 茶叶多糖及其开发利用[J]. 中国食品添加剂, 2000, 2: 65~69.
[7] 汪东风, 谢晓凤, 王泽农, 等. 粗老茶中的多糖含量及其保健作用[J]. 茶叶科学, 1994, 14(1): 73~74