采用在茶树细胞培养过程中饲喂标记前体的方法,进行了14C标记儿茶素的合成研究。比较了14C-苯丙氨酸和14C-乙酸钠两种前体的饲喂效果,分析了饲喂前体被培养细胞吸收与利用的动态,以及外源苯丙氨酸饲喂所带来的细胞产物组成与水平变化,并采用高速逆流色谱和高效液相色谱技术对合成的标记产物进行了分离与分析。
The preparation of 14C labeled catechins was investigated by feeding 14C labeled precursor to tea cultured cells. Two labeled precursors were tested and their effects were compared. The dynamics of absorption and transformation of fed precursors and the changes of product composition and content caused by exogenous precursor were analyzed. Product analysis was also made by HSCCC and HPLC techniques.
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