利用外源前体饲喂生物合成14C-儿茶素的研究

成浩; 李素芳; 沈星荣; 孙锦荷

doi:10.13305/j.cnki.jts.2003.s1.016

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茶叶科学 ›› 2003, Vol. 23 ›› Issue (s1) : 82-87. DOI: 10.13305/j.cnki.jts.2003.s1.016

利用外源前体饲喂生物合成¹⁴C-儿茶素的研究

成浩^{1, 2}, 李素芳², 沈星荣², 孙锦荷¹

作者信息 +

Biosynthesis of ¹⁴C-catechins by Tea Cell Culture

CHENG Hao^{1, 2}, LI Su-fang2, SHEN Xing-rong², SUN Jin-he¹

Author information +

文章历史 +

摘要

采用在茶树细胞培养过程中饲喂标记前体的方法,进行了¹⁴C标记儿茶素的合成研究。比较了¹⁴C-苯丙氨酸和¹⁴C-乙酸钠两种前体的饲喂效果,分析了饲喂前体被培养细胞吸收与利用的动态,以及外源苯丙氨酸饲喂所带来的细胞产物组成与水平变化,并采用高速逆流色谱和高效液相色谱技术对合成的标记产物进行了分离与分析。

Abstract

The preparation of ¹⁴C labeled catechins was investigated by feeding ¹⁴C labeled precursor to tea cultured cells. Two labeled precursors were tested and their effects were compared. The dynamics of absorption and transformation of fed precursors and the changes of product composition and content caused by exogenous precursor were analyzed. Product analysis was also made by HSCCC and HPLC techniques.

导出引用

成浩, 李素芳, 沈星荣, 孙锦荷. 利用外源前体饲喂生物合成¹⁴C-儿茶素的研究[J]. 茶叶科学. 2003, 23(s1): 82-87 https://doi.org/10.13305/j.cnki.jts.2003.s1.016

CHENG Hao, LI Su-fang, SHEN Xing-rong, SUN Jin-he. Biosynthesis of ¹⁴C-catechins by Tea Cell Culture[J]. Journal of Tea Science. 2003, 23(s1): 82-87 https://doi.org/10.13305/j.cnki.jts.2003.s1.016

中图分类号： Q813.1

参考文献

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