观察茶儿茶素对铅诱导HepG2细胞氧化损伤和茶多酚对铅染毒小鼠体内铅含量的影响。采用MTT（四甲基偶氮唑盐）法检测细胞活力,TBA（硫代巴比妥酸）反应生成MDA（丙二醛）的量测量细胞脂质过氧化水平。以醋酸铅染毒小鼠,采用原子荧光光度法测定小鼠器官中的铅含量。结果发现儿茶素能改善铅诱导细胞的氧化损伤,使细胞活力升高,MDA下降;铅染毒后,小鼠血液、肝、肾、脑和股骨蓄积的铅含量显著增加,茶多酚对小鼠肝脏排铅有显著作用,但对其他器官的铅排泄无明显影响。
朴宰日
,
王岳飞
,
杨贤强
,
陈留记
,
杨勇
,
赵保路
,
焦鸿利
,
周淡宜
. 儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响[J]. 茶叶科学, 2003
, 23(2)
: 119
-123
.
DOI: 10.13305/j.cnki.jts.2003.02.007
The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.
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