为筛选用于黄金芽叶色黄化分子机理研究的适宜材料,采用双层遮荫（平均光强约10βklx）、单层遮荫（平均光强约40βklx）和不遮荫3种光照强度处理黄金芽春梢,获得嫩绿色（H_1W）、浅黄稍带绿色（H_4W）、明黄色（Hs）3种新梢,同时以福鼎大白绿色新梢（CK_f）为对照,选择芽下第二叶为材料,通过测定H₂O₂、O₂^-含量和组织化学定位、抗氧化物酶活性、光合响应曲线、叶绿素荧光等叶片生理指标,观测叶绿体膜系统结构。结果表明,浅黄稍带绿叶片H_4W的H₂O₂、O₂^-含量、Fv/Fm值和叶绿体膜系统情况与嫩绿色叶片H_1W相近,处于未受逆境胁迫的生理状态;同时浅黄叶片H_4W与明黄叶片Hs具有相似的黄化叶光合生理特性,两者在ΦPSⅡ、qP、光饱和点、光补偿点等叶绿素荧光参数和光合指标上差异不显著,光响应曲线特征相似。由于浅黄稍带绿叶片（H_4W）所具有的上述生理特点,推测其是探究黄金芽茶树品种黄化分子机理必不可少的研究材料。

In order to screen suitable materials for studying the molecular mechanism of ‘Huangjinya’, the spring shoots of ‘Huangjinya’ were treated with double shading (average light intensity of 10βklx), single shading (average light intensity of 40βklx) and non-shading. And three new shoots, light green (H_1W), light yellow (H_4W) and bright yellow (Hs), were obtained. At the same time, the second leaf under bud was selected as material with ‘Fudingdabai’ green shoot (CK_f) as control. The content of hydrogen peroxide, oxygen-and histochemical localization, antioxidant enzyme activity, photosynthetic response curve, chlorophyll fluorescence and other physiological indicators of leaves were measured and the structure of chloroplast membrane system was observed. The results show that The H₂O₂, O₂^- contents, Fv/Fm value and chloroplast membrane system of light yellow leaf H_4W were similar to those of light green leaf H_1W, which were in a stress-free state. At the same time, the photosynthetic and physiological characteristics of light yellow leaf H_4W and bright yellow leaf Hs were similar. There were no significant differences in chlorophyll fluorescence parameters and photosynthetic indexes such as ΦPSⅡ, qP, light saturation point and light compensation point, and the characteristics of light response curve were similar. Because of the above physiological characteristics of light yellow leaf H_4W, it could be used as the research material to explore the molecular mechanism of yellowing of golden bud tea varieties.