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茶叶科学 ›› 2019, Vol. 39 ›› Issue (5): 530-536.doi: 10.13305/j.cnki.jts.2019.05.004

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黄金芽不同色泽叶片生理特性研究

范延艮, 赵秀秀, 王翰悦, 田月月, 向勤锃, 张丽霞*   

  1. 山东农业大学园艺科学与工程学院,山东 泰安 271018
  • 收稿日期:2019-03-13 修回日期:2019-05-01 出版日期:2019-10-15 发布日期:2019-10-15
  • 通讯作者: *zlx_sdau@163.com
  • 作者简介:范延艮,男,博士研究生,主要研究方向为茶树生理与生态,876562801@qq.com。
  • 基金资助:
    山东“双一流”奖补资金(515563001)

Study on Physiological Characteristics of Leaves with Different Colors of ‘Huangjinya’

FAN Yangen, ZHAO Xiuxiu, WANG Hanyue, TIAN Yueyue, XIANG Qinzeng, ZHANG Lixia*   

  1. Shandong Agricultural University, Tai'an 271017, China
  • Received:2019-03-13 Revised:2019-05-01 Online:2019-10-15 Published:2019-10-15

摘要: 为筛选用于黄金芽叶色黄化分子机理研究的适宜材料,采用双层遮荫(平均光强约10βklx)、单层遮荫(平均光强约40βklx)和不遮荫3种光照强度处理黄金芽春梢,获得嫩绿色(H1W)、浅黄稍带绿色(H4W)、明黄色(Hs)3种新梢,同时以福鼎大白绿色新梢(CKf)为对照,选择芽下第二叶为材料,通过测定H2O2、O2-含量和组织化学定位、抗氧化物酶活性、光合响应曲线、叶绿素荧光等叶片生理指标,观测叶绿体膜系统结构。结果表明,浅黄稍带绿叶片H4W的H2O2、O2-含量、Fv/Fm值和叶绿体膜系统情况与嫩绿色叶片H1W相近,处于未受逆境胁迫的生理状态;同时浅黄叶片H4W与明黄叶片Hs具有相似的黄化叶光合生理特性,两者在ΦPSⅡqP、光饱和点、光补偿点等叶绿素荧光参数和光合指标上差异不显著,光响应曲线特征相似。由于浅黄稍带绿叶片(H4W)所具有的上述生理特点,推测其是探究黄金芽茶树品种黄化分子机理必不可少的研究材料。

关键词: 黄金芽, 光氧化胁迫, 光照强度, 黄化材料

Abstract: In order to screen suitable materials for studying the molecular mechanism of ‘Huangjinya’, the spring shoots of ‘Huangjinya’ were treated with double shading (average light intensity of 10βklx), single shading (average light intensity of 40βklx) and non-shading. And three new shoots, light green (H1W), light yellow (H4W) and bright yellow (Hs), were obtained. At the same time, the second leaf under bud was selected as material with ‘Fudingdabai’ green shoot (CKf) as control. The content of hydrogen peroxide, oxygen-and histochemical localization, antioxidant enzyme activity, photosynthetic response curve, chlorophyll fluorescence and other physiological indicators of leaves were measured and the structure of chloroplast membrane system was observed. The results show that The H2O2, O2- contents, Fv/Fm value and chloroplast membrane system of light yellow leaf H4W were similar to those of light green leaf H1W, which were in a stress-free state. At the same time, the photosynthetic and physiological characteristics of light yellow leaf H4W and bright yellow leaf Hs were similar. There were no significant differences in chlorophyll fluorescence parameters and photosynthetic indexes such as ΦPSⅡ, qP, light saturation point and light compensation point, and the characteristics of light response curve were similar. Because of the above physiological characteristics of light yellow leaf H4W, it could be used as the research material to explore the molecular mechanism of yellowing of golden bud tea varieties.

Key words: Camellia sinensis cv. Huangjinya, photooxidation stress, light-intensity, chlorotic material

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