茶鲜叶挥发物组分及对茶树病原菌的熏蒸抑制作用

智亚楠; 徐运飞; 朱明星; 王春生; 金银利; 陈利军

doi:10.13305/j.cnki.jts.2021.03.005

茶叶科学 >

2021 , Vol. 41 >Issue 3: 371 - 378

DOI: https://doi.org/10.13305/j.cnki.jts.2021.03.005

研究报告

茶鲜叶挥发物组分及对茶树病原菌的熏蒸抑制作用

智亚楠 ,
徐运飞 ,
朱明星 ,
王春生 ,
金银利 ,
陈利军

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1.信阳农林学院农学院,河南信阳 464000;
2.河南省豫南农作物有害生物绿色防控院士工作站,河南信阳 464000

智亚楠,女,讲师,主要从事植物源农药与农药剂型研究,zhiyanan000@126.com。

收稿日期: 2020-06-08

修回日期: 2020-07-28

网络出版日期: 2021-06-15

基金资助

茶园化肥农药减施增效技术集成研究与示范（2016YFD0200900）、信阳农林学院科技创新团队项目（KJCXTD-201903）、河南省科技攻关项目（162102110085）

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Volatile Organic Compounds from Fresh Tea Leaves and Their Fumigation Antifungal Activities on Fungal Pathogens of Tea Plants

ZHI Yanan ,
XU Yunfei ,
ZHU Mingxing ,
WANG Chunsheng ,
JIN Yinli ,
CHEN Lijun

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1. Agriculture College, Xinyang Agriculture and Forestry University, Xinyang 464000, China;
2. Henan Provincial South Henan Crop Pest Green Prevention and Control Academician Workstation, Xinyang 464000, China

Received date: 2020-06-08

Revised date: 2020-07-28

Online published: 2021-06-15

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摘要

为明确茶鲜叶中的挥发物组分及其与茶树主要病原菌之间的关系,利用HS-SPME-GC-MS法萃取分析茶鲜叶挥发物组分,并以茶褐枯病菌、茶云纹叶枯病菌、茶炭疽病菌、茶轮斑病菌为目标菌,测定茶鲜叶主要挥发物单体组分对病原菌的熏蒸抑制作用。结果显示,从茶鲜叶挥发物中分离到28个组分,鉴定出其中19个组分,占挥发物总量的94.405%,其主要组分为(Z)-己酸-3-己烯酯（18.395%）、乙酸叶醇酯（16.935%）、罗勒烯（12.615%）和顺-3-己烯基丁酯（11.210%）,其中顺-3-己烯基丁酯对4种病原菌均具有熏蒸抑制作用,EC₅₀均低于61.29 μL·L^-1。结果表明,顺-3-己烯基丁酯有作为熏蒸型杀菌剂应用于茶树病害防控的潜力。

关键词： 茶鲜叶; 熏蒸; 抑菌活性; 挥发物; 顺-3-己烯基丁酯

本文引用格式

智亚楠 , 徐运飞 , 朱明星 , 王春生 , 金银利 , 陈利军 . 茶鲜叶挥发物组分及对茶树病原菌的熏蒸抑制作用[J]. 茶叶科学, 2021 , 41(3) : 371 -378 . DOI: 10.13305/j.cnki.jts.2021.03.005

Abstract

In order to determine the relationship between volatile organic compounds (VOCs) from fresh tea leaves and the main fungal pathogens of tea plants, the VOCs from fresh tea leaves were extracted and analyzed by HS-SPME-GC-MS. The fumigation antifungal activities of volatile monomers from fresh tea leaves against four fungal pathogens including Botryosphaeria dothidea, Colletotrichum camelliae, Colletotrichum gloeosporioides, Pestalotiopsis theae were also studied. The results show that 28 chemical compounds were found from VOCs of fresh tea leaves, and 19 of them were characterized, accounting for 94.405% of the total VOCs. The main chemical compounds were hexanoic acid, 3-hexenyl ester, (Z)-hexanoic acid-3-hexenyl ester (18.395%), leaf acetate (16.935%), ocimene (12.615%), and cis-3-hexenyl butyrate (11.210%). Cis-3-hexenyl butyrate exhibited strong fumigation antifungal effect on the four tested fungal pathogens, and the EC₅₀ were lower than 61.29 μL·L^-1. In conclusion, cis-3-hexenyl butyrate can be used as a potential fumigant control of tea diseases.

Key words： fresh tea leaves; fumigation; antifungal activity; VOCs; cis-3-hexenyl butyrate

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