一株云南木霉菌培养条件的优化及其对茶炭疽病的防控研究

刘辉; 冯月玲; 朱秀英; 郑周洲; 刘思睿; 周罗娜; 潘雪珍; 宋莉

doi:10.13305/j.cnki.jts.2024.04.013

茶叶科学 >

2024 , Vol. 44 >Issue 4: 627 - 638

DOI: https://doi.org/10.13305/j.cnki.jts.2024.04.013

研究报告

一株云南木霉菌培养条件的优化及其对茶炭疽病的防控研究

刘辉 ,
冯月玲 ,
朱秀英 ,
郑周洲 ,
刘思睿 ,
周罗娜 ,
潘雪珍 ,
宋莉

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1.贵州省农业科学院生物技术研究所/贵州省农业生物技术重点实验室,贵州贵阳 550009;
2.贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,贵州贵阳 550025;
3.贵州师范学院,贵州贵阳 550018;
4.贵州发来地农业科技有限公司,贵州龙里 551202

刘辉,男,副研究员,主要从事作物栽培生理学和作物病害绿色防控方面的研究。

收稿日期: 2023-12-09

修回日期: 2024-03-24

网络出版日期: 2024-09-03

基金资助

贵州省科技支撑计划（黔科合支撑[2021]一般192、黔科合支撑[2023]一般037）、黔南州科技支撑计划（黔南科合[2022]01号）

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Optimization of Culture Conditions of A Trichoderma yunnanensis and Its Control Efficiency of Tea Anthracnose

LIU Hui ,
FENG Yueling ,
ZHU Xiuying ,
ZHENG Zhouzhou ,
LIU Sirui ,
ZHOU Luona ,
PAN Xuezhen ,
SONG Li

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1. Institute of Biotechnology, Guizhou Academy of Agricultural Sciences/Guizhou Provincial Key Laboratory of Agricultural Biotechnology, Guiyang 550009, China;
2. Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;
3. Guizhou Education University, Guiyang 550018, China;
4. Guizhou Falaidi Agricultural Technology Co., Ltd., Longli 551202, China

Received date: 2023-12-09

Revised date: 2024-03-24

Online published: 2024-09-03

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摘要

为优化对茶炭疽病菌Colletotrichum camelliae有拮抗作用的云南木霉菌Trichodema yunnanense的发酵培养条件,并明确发酵液对茶炭疽病的防控效果,以对C. camelliae的抑菌率为评价指标,通过单因素和响应面试验,优化云南木霉菌的发酵条件并获得发酵液,比较其对茶炭疽病的抑菌活性、离体叶片和盆栽茶树叶片防效及茶树生长的影响。结果显示,T. yunnanense的最佳发酵条件为马铃薯200βg·L^-1、甘露醇18.85βg·L^-1、酵母浸膏4.73βg·L^-1、装液量372.60βmL·L^-1、培养温度25β℃、pH 6.6、12L∶12D。10%的发酵液对C. camelliae的抑菌率达92.61%,对感染茶炭疽病的离体茶树叶片与盆栽茶树叶片的防效分别为63.71%与68.95%,均显著高于哈茨木霉菌（T. harzianum）可湿性粉剂与多菌灵的防效。同时,10%发酵液处理后的茶树幼苗根长提升69.16%,根鲜重提升215.70%,株高增加42.13%,地上部鲜重增加212.11%。表明T. yunnanense发酵液兼具抗菌和促生作用。研究结果为T. yunnanense在茶炭疽病生物防治中的应用奠定了一定的理论基础。

关键词： 云南木霉菌; 茶炭疽病; 发酵液; 响应面; 生物防控

本文引用格式

刘辉 , 冯月玲 , 朱秀英 , 郑周洲 , 刘思睿 , 周罗娜 , 潘雪珍 , 宋莉 . 一株云南木霉菌培养条件的优化及其对茶炭疽病的防控研究[J]. 茶叶科学, 2024 , 44(4) : 627 -638 . DOI: 10.13305/j.cnki.jts.2024.04.013

Abstract

To optimize the fermentation conditions of Trichoderma yunnanensis, which has antagonistic effect on Colletotrichum camelliae, single factor and response surface tests were performed with the inhibition rate of C. camelliae as the evaluation index. Then, the antagonistic activity, in vitro leaf control effect, indoor pot control effect, and growth effect of the fermentation broth on C. camelliae and tea plants, respectively, were determined. The results show that the optimum fermentation conditions of T. yunnanense were as follows: potato 200βg·L^-1, mannitol 18.85βg·L^-1, yeast extract 4.73βg·L^-1, liquid content 372.60βmL·L^-1, culture temperature 25β℃, pH 6.6, 12L∶12D. The inhibitory rate of the obtained fermentation broth on C. camelliae reached 92.61% under the ratio of 1∶9 mixed with potato broth (PDA) medium. The control effect of the fermentation broth on anthrax in vitro leaf was 63.71% and that on indoor potted tea plants was 68.95%, both of which were significantly higher than that of T. harzianum wettable powder and carbendazim. Compared with the water control, the growth of tea seedlings treated with fermentation solution of T. yunnensis was significantly improved, which was manifested by the increase of root length, root fresh weight, plant height and above ground fresh weight by 69.16%, 215.70%, 42.13% and 212.11% respectively. Overall, T. yunnanense fermentation liquid has both antibiotic and growth-promoting effects. The results provided a theoretical basis for the application of T. yunnanense fermentation broth in the biological control of tea anthracnose.

Key words： Trichoderma yunnanensis; tea anthracnose; fermentation broth; response surface method; biological control

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