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渥堆发酵对六堡茶低聚糖的化学组成及抗氧化活性影响

  • 唐芷琦 ,
  • 秦寒傲 ,
  • 夏宁 ,
  • 滕建文 ,
  • 黄丽 ,
  • 欧筱瑄 ,
  • 陈丽萍 ,
  • 韦玮 ,
  • 王瑞
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  • 广西大学轻工与食品工程学院,广西 南宁 530004
唐芷琦,女,硕士研究生,主要从事食品功能性成分与食品安全方面的研究。

收稿日期: 2024-12-13

  修回日期: 2025-01-23

  网络出版日期: 2025-06-18

基金资助

国家自然科学基金(32160571)、广西创新训练项目(S202410593148)

The Effect of Pile-fermentation on the Chemical Composition and Antioxidant Activities of Liupao Tea Oligosaccharides

  • TANG Zhiqi ,
  • QIN Han'ao ,
  • XIA Ning ,
  • TENG Jianwen ,
  • HUANG Li ,
  • OU Xiaoxuan ,
  • CHEN Liping ,
  • WEI Wei ,
  • WANG Rui
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  • College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China

Received date: 2024-12-13

  Revised date: 2025-01-23

  Online published: 2025-06-18

摘要

为探讨渥堆过程对六堡茶低聚糖的结构特征及抗氧化活性的影响,选取同一批次的毛茶原料与渥堆后的六堡茶,利用水提醇沉法制得粗低聚糖,通过大孔吸附树脂与葡聚糖凝胶LH-20色谱柱分离纯化得到毛茶低聚糖RLTO-1与渥堆后六堡茶低聚糖ALTO-2,并采用化学分析、凝胶渗透色谱、离子交换色谱、红外光谱、紫外可见分光光度计分析其化学组成;通过测定其ABTS自由基清除能力、DPPH自由基清除能力及总抗氧化能力,评价发酵前后低聚糖的体外抗氧化能力的差异。结果显示,RLTO-1与ALTO-2的总糖含量分别为57.30%与63.98%,多酚含量分别为11.77%与22.28%,糖醛酸含量分别为3.49%与18.25%。RLTO-1含2个组分,分子质量分别为1 565 Da和607 Da,主要由葡萄糖、阿拉伯糖、核糖等单糖构成;ALTO-2的分子质量为833 Da,主要由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、半乳糖醛酸等单糖构成。RLTO-1是一类具有αβ构型和非还原性吡喃环的杂低聚糖,ALTO-2是具有α-构型和非还原性吡喃环的杂低聚糖。体外抗氧化研究结果表明,发酵前后的低聚糖均具有抗氧化活性,ALTO-2相较于RLTO-1表现出更好的抗氧化能力。研究表明,六堡茶低聚糖经过渥堆发酵后,其分子质量、单糖组成与结构均发生明显改变,具有更强的抗氧化活性,为六堡茶低聚糖相关功能产品的开发提供理论基础。

本文引用格式

唐芷琦 , 秦寒傲 , 夏宁 , 滕建文 , 黄丽 , 欧筱瑄 , 陈丽萍 , 韦玮 , 王瑞 . 渥堆发酵对六堡茶低聚糖的化学组成及抗氧化活性影响[J]. 茶叶科学, 2025 , 45(3) : 509 -521 . DOI: 10.13305/j.cnki.jts.20250421.001

Abstract

To investigate the effect of pile-fermentation of Liupao tea on the structural characteristics and antioxidant capacities of oligosaccharides, the same batch of the raw tea and Liupao tea after fermentation were selected in this study. The raw tea oligosaccharide RLTO-1 and the oligosaccharide ALTO-2 of Liupao tea after fermentation were separated and purified by water extraction and alcohol precipitation, macroporous adsorption resin and dextran gel LH-20 column. The chemical composition was analyzed by chemical analysis, gel penetration chromatography, ion exchange chromatography, infrared spectroscopy and UV visible spectrophotometer. The differences in in vitro antioxidant capacities of oligosaccharides before and after fermentation were evaluated by measuring their ABTS radical scavenging capacity, DPPH radical scavenging capacity and total antioxidant capacity. The results show that the total sugar contents of RLTO-1 and ALTO-2 were 57.30% and 63.98%, and the polyphenol contents were 11.77% and 22.28%, respectively. The contents of uronic acid were 3.49% and 18.25%, respectively. RLTO-1 contains two components with molecular weights of 1 565 Da and 607 Da, primarily composed of glucose, arabinose, ribose and other monosaccharides. ALTO-2 has a molecular weight of 833 Da and is mainly composed of monosaccharides such as rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid. RLTO-1 is a class of hetero-oligosaccharide with α and β configurations and non-reducing pyran rings, and ALTO-2 is a hetero-oligosaccharide with a non-reducing pyran ring with a α-configuration. The results of in vitro antioxidant studies show that the oligosaccharides before and after fermentation had antioxidant activity, and ALTO-2 shows better antioxidant capacity compared with RLTO-1. This indicates that the molecular weight, monosaccharide composition and structure of Liupao tea oligosaccharides are significantly changed after heap fermentation, and have stronger antioxidant activities, which provide a theoretical basis for the development of Liupao tea oligosaccharides.

参考文献

[1] Sako T, Matsumoto K, Tanaka R.Recent progress on research and applications of non-digestible galacto-oligosaccharides[J]. International Dairy Journal, 1999, 9(1): 69-80.
[2] 梁珊, 李小凤, 廖文镇, 等. 功能性低聚糖分离纯化研究进展[C]//广东省食品学会. “健康与安全”学术研讨会暨2015年广东省食品学会年会论文集. 广州: 广东省食品学会, 2015: 5.
Liang S, Li X F, Liao W Z, et al.Research progress in separation and purification of functional oligosaccharides[C]//Guangdong Food Society. “Health and Safety” Academic Seminar and 2015 Guangdong Food Society Annual Meeting Proceedings. Guangzhou: Guangdong Food Society, 2015: 5.
[3] Kaur A P, Bhardwaj S, Dhanjal D S, et al.Plant prebiotics and their role in the amelioration of diseases[J]. Biomolecules, 2021, 11(3): 440. doi: 10.3390/biom11030440.
[4] Nattga P, Santad W, Pritsana R, et al.Inflammatory response of raw 264.7 macrophage cells teated with dragon fruit oligosaccharide on lipopolysaccharide-induced inflammation[J]. Food Science and Technology Research, 2021, 27(1): 111-119.
[5] Deng J F, Ran Y H.The primary antitumor activities study of a novel peach gum oligosaccharide[J]. Transactions on Cancer, 2020, 2: 1-6.
[6] Ackerman D L, Craft K M, Doster R S, et al.Antimicrobial and antibiofilm activity of human milk oligosaccharides against Streptococcus agalactiae, Staphylococcus aureus, and Acinetobacter baumannii[J]. ACS Infectious Diseases, 2018, 4(3): 315-324.
[7] Huang S Y, Chen H, Teng J W, et al.Antihyperlipidemic effect and increased antioxidant enzyme levels of aqueous extracts from Liupao tea and green tea in vivo[J]. Journal of Food Science, 2022, 87(9): 4203-4220.
[8] Ding Q Z, Zhang B W, Zheng W, et al.Liupao tea extract alleviates diabetes mellitus and modulates gut microbiota in rats induced by streptozotocin and high-fat, high-sugar diet[J]. Biomedicine & Pharmacotherapy, 2019, 118: 109262. doi: 10.1016/j.biopha.2019.109262.
[9] 聂晴, 庞月兰, 吴焕, 等. 陈年六堡茶对Aβ25-35诱导的PC12细胞损伤的神经保护研究[J]. 茶叶科学, 2024, 44(6): 1005-1013.
Nie Q, Pang Y L, Wu H, et al.Neuroprotective mechanisms of aged Liupao tea against Aβ25-35-induced PC12 cell damage[J]. Journal of Tea Science, 2024, 44(6): 1005-1013.
[10] Gong Z P, Ouyang J, Wu X L, et al.Dark tea extracts: chemical constituents and modulatory effect on gastrointestinal function[J]. Biomedicine & Pharmacotherapy, 2020, 130: 110514. doi: 10.1016/j.biopha.2020.110514.
[11] 刘仲华, 黄建安, 施兆鹏. 黑茶初制中主要酶类的变化[J]. 茶叶科学, 1991, 11(s1): 17-22.
Liu Z H, Huang J A, Shi Z P.Dynamics of the major enzymes during the primary processing of dark green tea[J]. Journal of Tea Science, 1991, 11(s1): 17-22.
[12] Pang B W, Huang L, Teng J W, et al.Effect of pile fermentation on the cells of Chinese Liupao tea: the first record of cell wall of Liupao tea on transmission electron microscope[J]. Food Chemistry, 2021, 361: 130034. doi: 10.1016/J.foodchem.2021.130034.
[13] Feng X, Deng H C, Huang L, et al.Degradation of cell wall polysaccharides during traditional and tank fermentation of Chinese Liupao tea[J]. Journal of Agricultural and Food Chemistry, 2024, 72(8): 4195-4206.
[14] Liu S, Hu J L, Zhong Y D, et al.A review: effects of microbial fermentation on the structure and bioactivity of polysaccharides in plant-based foods[J]. Food Chemistry, 2023, 440: 137453. doi: 10.1016/j.foodchem.2023.137453.
[15] Xiang G, Sun H P, Chen Y Y, et al.Antioxidant and hypoglycemic activity of tea polysaccharides with different degrees of fermentation[J]. International Journal of Biological Macromolecules, 2022, 228: 224-233.
[16] Dubois M, Gilles K A, Hamilton J K, et al.Colorimetric method for determination of sugars and related substances[J]. Analytical Chemistry, 1956, 28(3): 350-356.
[17] Kumazawa S, Hamasaka T, Nakayama T.Antioxidant activity of propolis of various geographic origins[J]. Food Chemistry, 2004, 84(3): 329-339.
[18] 姜瑞芝, 陈英红, 杨勇杰, 等. 银耳多糖中糖醛酸含量的测定[J]. 中草药, 2004(9): 36-38.
Jiang R Z, Chen Y H, Yang Y J, et al.Determination of uronic acids in polysaccharide from Tremella fuciformis[J]. Chinese Traditional and Herbal Drugs, 2004(9): 36-38.
[19] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1/2): 248-254.
[20] Wang L, Zhang B, Xiao J, et al.Physicochemical, functional, and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit[J]. Food Chemistry, 2018, 249: 127-135.
[21] Dahmoune F, Nayak B, Moussi K, et al.Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves[J]. Food Chemistry, 2015, 166: 585-595.
[22] Liu A L, Huang B H, Lei L, et al.Production of high antioxidant activity flavonoid monoglucosides from citrus flavanone with immobilised α-L-rhamnosidase in one step[J]. International Journal of Food Science & Technology, 2019, 54(10): 2854-2862.
[23] 周杨, 胡小静, 周红杰, 等. 云南普洱茶水溶性碳水化合物的变化[J]. 湖南农业大学学报(自然科学版), 2006(6): 625-627.
Zhou Y, Hu X J, Zhou H J, et al.Changes of water soluble carbohydrates in different Yunnan Pu-erh tea[J]. Journal of Hunan Agricultural University (Natural Sciences), 2006(6): 625-627.
[24] Le Bourvellec C, Renard C M G C. Interactions between polyphenols and macromolecules: quantification methods and mechanisms[J]. Critical Reviews in Food Science and Nutrition, 2012, 52(3): 213-248.
[25] Zhang H L, Li J, Xia J M, et al.Antioxidant activity and physicochemical properties of an acidic polysaccharide from Morinda officinalis[J]. International Journal of Biological Macromolecules, 2013, 58: 7-12.
[26] Zhang Z J, Wang F H, Wang M C, et al.A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge[J]. Carbohydrate Polymers, 2015, 117: 980-987.
[27] Xu P, Chen H, Wang Y Q, et al.Oral administration of puerh tea polysaccharides lowers blood glucose levels and enhances antioxidant status in alloxan-induced diabetic mice[J]. Journal of Food Science, 2012, 77(11): H246-H252.
[28] 龚加顺, 胡小静, 彭春秀, 等. 普洱茶及其原料多糖分子组成及光谱学特性研究[J]. 光谱学与光谱分析, 2010, 30(7): 1960-1964.
Gong J S, Hu X J, Peng C X, et al.The molecular composition and spectral properties of polysaccharide isolated from pu-erh tea and its material[J]. Spectroscopy and Spectral Analysis, 2010, 30(7): 1960-1964.
[29] Liu Y M, Liu W, Li J, et al.A polysaccharide extracted from Astragalus membranaceus residue improves cognitive dysfunction by altering gut microbiota in diabetic mice[J]. Carbohydrate Polymers, 2018, 205: 500-512.
[30] Wioleta W, Anna K, Lucyna D, et al.Fibres from flax overproducing β-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity[J]. BMC Biotechnology, 2013, 13(1): 10. doi: 10.1186/1472-6750-13-10.
[31] Phukan M M, Chutia R S, Konwar B K, et al.Microalgae chlorella as a potential bio-energy feedstock[J]. Applied Energy, 2010, 88(10): 3307-3312.
[32] Xu P, Wu J, Zhang Y, et al.Physicochemical characterization of puerh tea polysaccharides and their antioxidant and α-glycosidase inhibition[J]. Journal of Functional Foods, 2014, 6: 545-554.
[33] Guo H, Fu M X, Wu D, et al.Structural characteristics of crude polysaccharides from 12 selected Chinese teas, and their antioxidant and anti-diabetic activities[J]. Antioxidants, 2021, 10(10): 1562. doi: 10.3390/antiox10101562.
[34] Yuan Q, Lin S, Fu Y, et al.Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus)[J]. International Journal of Biological Macromolecules, 2019, 127: 178-186.
[35] 李焱, 林泳峰, 刘文美, 等. 茶多糖研究的现状与发展趋势[J]. 茶叶科学, 2023, 43(4): 447-459.
Li Y, Lin Y F, Liu W M, et al.Present status and development trends of research on tea polysaccharides[J]. Journal of Tea Science, 2023, 43(4): 447-459.
[36] Yan J K, Wu L X, Qiao Z R, et al.Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices[J]. Food Chemistry, 2019, 271: 588-596.
[37] Liang J, Zhao Y L, Yang F R, et al.Preparation and structure-activity relationship of highly active black garlic polysaccharides[J]. International Journal of Biological Macromolecules, 2022, 220: 601-612.
[38] Wei Q, Zhang Y H.Ultrasound-assisted polysaccharide extraction from Cercis chinensis and properites, antioxidant activity of polysaccharide[J]. Ultrasonics Sonochemistry, 2023, 96: 106422. doi: 10.1016/j.ultsonch.2023.106422.
[39] Zhang J Q, Li C, Huang Q, et al.Comparative study on the physicochemical properties and bioactivities of polysaccharide fractions extracted from Fructus Mori at different temperatures[J]. Food & Function, 2019, 10(1): 410-421.
[40] Liu G M, Sun J, He X M, et al.Fermentation process optimization and chemical constituent analysis on longan (Dimocarpus longan Lour.) wine[J]. Food Chemistry, 2018, 256: 268-279.
[41] Wu S H, Li F, Jia S Y, et al.Drying effects on the antioxidant properties of polysaccharides obtained from Agaricus blazei Murrill[J]. Carbohydrate Polymers, 2014, 103: 414-417.
[42] Li X, Wei J, Lin L Z, et al.Structural characterization, antioxidant and antimicrobial activities of polysaccharide from Akebia trifoliata (Thunb.) Koidz stem[J]. Colloids and Surfaces B: Biointerfaces, 2023, 231: 113573. doi: 10.1016/j.colsurfb.2023.113573.
[43] Tian L M, Zhao Y, Guo C, et al.A comparative study on the antioxidant activities of an acidic polysaccharide and various solvent extracts derived from herbal Houttuynia cordata[J]. Carbohydrate Polymers, 2010, 83(2): 537-544.
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