不同品种茶树新梢响应&#x0201c;倒春寒&#x0201d;的转录组分析

王君雅; 陈玮; 刘丁丁; 陈亮; 姚明哲; 马春雷

doi:10.13305/j.cnki.jts.2019.02.007

茶叶科学 >

2019 , Vol. 39 >Issue 2: 181 - 192

DOI: https://doi.org/10.13305/j.cnki.jts.2019.02.007

不同品种茶树新梢响应“倒春寒”的转录组分析

王君雅 ,
陈玮 ,
刘丁丁 ,
陈亮 ,
姚明哲 ,
马春雷

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1. 中国农业科学院茶叶研究所国家茶树改良中心/农业部茶树生物学与资源利用重点实验室,浙江杭州 310008;
2. 中国农业科学院研究生院,北京 100081

王君雅,女,硕士研究生,主要从事茶树资源育种与遗传改良研究,wjy_8995@163.com。

收稿日期: 2019-01-07

网络出版日期: 2019-06-13

基金资助

中国农业科学院科技创新工程（CAAS-ASTIP-TRICAAS）、国家茶叶产业技术体系（CARS-19）、国家自然科学基金（31100504、31170624）、浙江省公益技术研究农业项目（2016C32024）、浙江省农业（茶树）新品种选育重大科技专项子课题（2016C02053-5）、中国农业科学院茶叶研究所基本科研业务费（1610212017008、1610212016002）

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The Transcriptome Analysis of Different Tea Cultivars in Response to the Spring Cold Spells

WANG Junya ,
CHEN Wei ,
LIU Dingding ,
CHEN Liang ,
YAO Mingzhe ,
MA Chunlei

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1. Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agriculture Science, Beijing 100081, China

Received date: 2019-01-07

Online published: 2019-06-13

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摘要

为探究“倒春寒”冻害对不同品种茶树新梢转录水平的影响,本研究以茶园“倒春寒”发生时受冻害和未受冻害的龙井43和中茶126新梢为研究材料,对两组样品进行转录组分析,分别鉴定到1 012个和1 079个差异基因,以及284个共同差异基因。从中选取18个差异基因进行实时荧光定量PCR验证,结果与转录组测序结果基本一致,证明转录组数据可靠。利用GO和KEGG数据库,对差异基因进行代谢通路富集分析,发现两个品种的差异基因主要集中在光合作用、碳代谢和细胞色素P450等代谢进程,说明“倒春寒”对新梢生长发育相关的基础代谢造成了严重损伤,抑制了相关基因的正常表达;随后对284个共同差异基因进行表达聚类分析,结果表明,其中99个差异基因在两个茶树品种中的表达模式完全相反,涉及的生物过程包括MAPK信号通路、谷胱甘肽和苯丙烷代谢等,推测这些差异基因与龙井43和中茶126在受到低温胁迫后产生的不同信号传导模式有关。

关键词： 茶树; 倒春寒; 转录组; 差异基因

本文引用格式

王君雅 , 陈玮 , 刘丁丁 , 陈亮 , 姚明哲 , 马春雷 . 不同品种茶树新梢响应“倒春寒”的转录组分析[J]. 茶叶科学, 2019 , 39(2) : 181 -192 . DOI: 10.13305/j.cnki.jts.2019.02.007

Abstract

In order to explore the effect of freezing damage caused by spring cold spells on the transcription profiling of young shoots of different tea cultivars, transcriptome analysis of the frozen and unfrozen shoots was performed on tea cultivar ‘Longjing 43’ and ‘Zhongcha 126’ during the cold spells. A total of 1 012 and 1 079 differentially expressed genes (DEGs) were identified in two cultivars with 284 DEGs overlapped. Then, eighteen DEGs were selected for Real-time quantitative PCR verification. The results were consistent with the transcriptome sequencing results, indicating that the transcriptome data was reliable. Utilizing the GO and KEGG pathway databases, the enriched pathways of the DEGs were identified, which included photosynthesis, carbon metabolism and cytochrome P450. This indicates that the cold spells cause serious damage to the basal metabolism related to the normal growth and development of young tea shoots and inhibit the expression of related genes. Subsequently, the expression cluster analysis of 284 overlapped DEGs showed that 99 DEGs exhibited the opposite expression patterns in two cultivars. The involved biological processes included MAPK signaling pathway, glutathione and phenylpropanoid metabolism. It was speculated that these DEGs were related to different signal transduction patterns in ‘Longjing 43’ and ‘Zhongcha 126’ in response to low temperature stress.

Key words： tea plant; cold spells; transcriptome; differentially expressed genes

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