采用脂代谢紊乱模型法—预防性给予不同地区不同剂量的红茶（剂量为成人每日饮用量的5倍、10倍和20倍3种）,观察小鼠体重、血脂、相关酶及肝脏形态的变化。结果表明：与高脂模型对照组相比,红茶剂量组能极显著（P<0.01）降低血清总胆固醇（TC）、低密度脂蛋白（LDL-C）、肝脏丙二醛（MDA）、肝脏指数;极显著（P<0.01）升高血清载脂蛋白A1（apoA1）、脂蛋白脂酶（LPL）、肝脂酶（HL）、总脂酶、肝脏超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GPX）,血清高密度脂蛋白（HDL-C）有所上升,小鼠体重也得到了一定的抑制,肝脏病变程度得到了相应的减轻,说明红茶具有一定的调节血脂及减小肝脏因高脂饮食所带来的损伤。
：The lipid metabolic disturbance model method was used to investigate the regulatory effects of Black Tea (BT) at different dosages (5 times, 10 times and 20 times of adult daily consumption) on serum lipid levels in hyperlipoidemia mice. In present study, blood lipid levels, body weight, and related enzymes of liver and serum were measured, and the histopathological changes in tissues of liver were also examined. It was found that the serum levels of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C), liver malondialdehyde (MDA) and liver index were significantly lower (P<0.01) in BT-treated mice compared with the high hyperlipoidemia model mice, while serum levels of apoA1, lipoprotein lipase (LPL), hepatictriglyceridelipase (HL) and total lipase, and liver superoxide dismutase (SOD) actives and glutathione peroxidase (GSH-Px) actives were significantly higher after treatment with BT (P<0.01). Additionally, serum levels of high density lipoprotein cholesterol (HDL-C) was slightly increased. Moreoverr, body weight was reduced, and the liver lesions were attenuated to some degree in BT-treated mice. The results suggested that BT showed a positive effect on regulating the serum lipid and reducing the liver injury induced by high fat diet.
[1] Kuo K L, Weng M S, Chiang C T, et al. Comparative studies on hypolipidemic and growth suppressive effects of Oolong, black, Pu-erh and green tea leaves in rats[J]. J Agr Food Chem, 2005, 53: 480-489.
[2] 陈玉琼, 张伟, 倪德江, 等. 湖北青砖茶辅助降血脂作用及其抗氧化效果[J]. 茶叶科学, 2010, 30(2): 124-128.
[3] 傅冬和, 刘仲华, 黄建安, 等. 茯砖茶降脂功能成分研究[J]. 茶叶科学, 2012, 32(2): 217-223.
[4] 熊昌云, 彭远菊, 王兴华, 等. 普洱茶不同溶剂提取组分降脂减肥作用的比较研究[J]. 茶叶科学, 2012, 32(6): 543-551.
[5] 赵宝权, 邵宛芳, 刘家奇, 等. 六堡茶、黑茶茶粉和普洱(熟茶)茶粉对Wistar大鼠调节血脂及抗氧化功能的比较研究[J]. 云南农业大学学报, 2013, 28(2): 236-241.
[6] 刘安军, 郭丹霄, 刘慧慧, 等. 绿茶提取物的降血脂及减肥作用研究[J]. 现代食品科技, 2012, 128(6): 601-605.
[7] Wilgus T A, Koki A T, Zweifel B S, et a1. Inhibition of cutaneous ultraviolet light B-mediated inflammation and tumor formation with topical celecoxib treatment[J]. Mol Carcinog, 2003, 38(2): 49-58.
[8] Ya-tao Du, Xuan Wang, Xiao-dong Wu, et al. Keetnun black tea extract contains potent fatty acid synthase inhibitors and reduces food intake and body weight of rats via oral administration [J]. Journal of Enzyme Inhibition and Medicinal Chemistry, 2005, 20(4): 349-356.
[9] S De Vos, R De Schrijver.Lipid metabolism, intestinal fermentation and mineral absorption in rats consuming black tea[J]. Nutrition Research, 2003, 23: 527-537.
[10] Ali Alshatwi, Manal A Al Obaaid, Sahar A Al Sedairy, et al. Black and green tea improves lipid profile and lipid peroxidation parameters in Wistar rats feda high-cholesterol diet[J]. Physiol Biochem, 2011, 67(1): 95-104.
[11] Hiroyuki Fujita, Tomohide Yamagami.Extract of Black Tea (Pu-Ehr) Inhibits Postprandial Rise in Serum Cholesterol in Mice, and With Long Term Use Reduces Serum Cholesterol and Low Density Lipoprotein Levels and Renal Fat Weight in Rats[J]. Phytothe. Res, 2008, 22(10): 1275-1281.
[12] Subhra Karmakar, Dolan Das, Anasuya Maiti, et al. Black Tea Prevents High Fat Diet-induced Non-alcoholic Steatohepatitis[J]. Phytother Res, 2011, 25(7): 1073-1081.
[13] 保健食品检验与评价技术规范[M]. 北京: 中华人民共和国卫生部, 2003.
[14] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1997.
[15] 曹丹, 刘仲华, 黄建安, 等. 不同时间给予大红袍水提物对小鼠急性酒精性肝损伤的影响[J]. 茶叶科学, 2013, 33(4): 289-294.
[16] 徐叔云, 卞如濂, 陈修. 药理实验方法学[M]. 3版. 北京: 人民卫生出版社, 2002.
[17] 陈奇. 中药药理研究方法学[M]. 北京: 人民卫生出版社, 1996: 619.
[18] 赵文. 食品安全性评价[M]. 1版. 北京: 化学工业出版社, 2006.
[19] 赵水平. 大幅度降低胆固醇能更有效地防治冠心病[J]. 中华医学信息导报, 2006, 21(12): 22.
[20] Sto K Y, Cooper D, Tailor A, et al. Hypercholesterolemia promotes inflammation and microvascular dysfunction: Role of nitric oxide and superoxide[J]. Free Radic Bio Med, 2002, 33(8): 1026-1036.
[21] 陈忠, 马根山, 黄峻. 脂蛋白脂酶与血脂代谢及冠心病关系研究进展[J]. 心血管病学进展, 2005, 26(3): 320-322.
[22] 李亚舒张宇佳, 王钟, 等. 载脂蛋白ApoA-I和ApoB测定对于冠心病防治的临床意义[J]. 南京军医学院学报, 2002, 24(3): 180-182.
浙公网安备 33019902000101号 
