在山东日照东港区茶园茶树叶部发现一种新病害。感病部位缢缩,畸形,病斑褐色,坏死,命名为茶褐枯病。取病健交界处采用组织分离法共获得两种微生物,编号为RC4、RC3,分离率分别为67.1%和32.9%。结合微生物形态学、培养学、多基因序列分析和柯赫氏法则验证,明确该病害由围小丛壳菌山茶专化型（Glomerella cingulata f. sp. camelliae）和葡萄座腔菌（Botryosphaeria dothidea）复合侵染引起。这也是首次在茶树叶部发现B. dothidea的危害。
A new leaf disease of tea plant was found in Donggang district of Rizhao, Shandong Province. The infected parts were shrunken and malformed, turned brown, necrotic and named brown blight of tea. Two microorganisms were isolated from the edge of infected parts through tissue separation, and named as RC4 and RC3. Their separation rates were 67.1% and 32.9% respectively. According to morphology, culture characteristics, multi- sequence analysis and Koch's Postulates test, Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea were found to be the cause of disease. This was the first report that Botryosphaeria dothidea damaged the leaves of tea plant.
[1] 肖强. 茶树病虫害绿色防控技术与专业化防治模式[J]. 中国茶叶, 2012(5): 20-23.
[2] 谢思惠, 张丽霞, 刘峰, 等. 山东茶叶发展现状及研究进展[J]. 山东林业科技, 2007, 170(3): 104-107.
[3] 孙晓玲. 中国重要茶树叶部病害的研究现状及展望[J].中国茶叶, 2016, 12(12): 12-15.
[4] 詹三良. 皖南茶区三种主要病害发生及防治初探[J]. 茶叶通报, 2016(4): 175-176.
[5] 张强, 杨云祥, 唐方圆, 等. 茶树主要病害及防治措施研究[J]. 中国农业信息, 2015, 6: 80-81.
[6] 戚利潮, 张叶大. 茶树主要病害及其防治[J]. 茶叶, 2016, 42(1): 10-12.
[7] 蒲国涛, 张锡友, 胡春学, 等. 汉中市城固县茶园病害调查及防治对策[J]. 陕西农业科学, 2014(7): 82-85.
[8] 方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998: 122-145.
[9] 汤铱泠, 周国英, 李河, 等. 多基因序列鉴定油茶炭疽病原Colletotrichum boninense新种[J]. 热带作物学报, 2015, 36(5): 972-977.
[10] 姚晟伟, 谢悦, 李兴红. 葡萄溃疡病病菌Botryosphaeria dothidea的诱导产孢方法评价[J]. 中外葡萄与葡萄酒, 2011(9): 4-7.
[11] 刘威, 陈玉森, 刘伟, 等. 茶树炭疽病的病原鉴定及其生物特性的研究[J]. 宁德师范学院学报(自然科学版), 2013, 25(1): 1-5.
[12] White T J, Bruns T, Lee S, et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics [M]//Innis M A, Gelfand D H, Sninsky J J, et al. PCR Protocols, A Guide to Methods and Applications. New York: Academic Press, 1990: 315-322.
[13] Glass NL, Donaldson GC.Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Appl Environ Microbiol, 1995, 61: 1323-1330.
[14] Weir B S, Johnston P R, Damm U.The Colletotrichum gloeosporioides species complex[J]. Studies in Mycology, 2012, 73(1): 115-180.
[15] Carbone I, Kohn LM.A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia, 1999, 91: 553-556.
[16] 王璠. 桃流胶病菌Botryosphaeria spp.鉴定、分布、遗传多样性及PCR快速检测技术研究[D]. 武汉: 华中农业大学, 2012.
[17] 刘威, 袁丁, 叶乃兴, 等. 茶树炭疽病病原鉴定[J]. 南方农业报, 2017, 48(3): 448-453.
[18] 姜晓龙, 金磊磊, 陈辉辉, 等. 1株侵染蓖麻的葡萄座腔菌菌株鉴定[J]. 江苏农业科学, 2015, 43(2): 319-322.
[19] Slipper B, Wingfield M J.Botryosphaeriaceae as endophytes and latent pathogens of woody Plants:diversity, ecology and impact[J]. Fungal Biological Reviews, 2007, 21: 90-106.
[20] 徐成楠, 迟福梅, 冀志蕊, 等. 蓝莓葡萄座腔菌枝枯病研究进展[J]. 中国果树, 2014(5): 71-74.
[21] 朱琪丽, 赵会长, 谢甲涛, 等. 葡萄座腔菌侵染柑橘果实的报道[J]. 植物病理学报, 2017, 44(9): 1-6.
[22] 李文英, 李夏, 解开治, 等. 葡萄座腔菌科真菌的系统性和多样性探讨[J]. 生物多样性, 2017, 25(8): 874-885.
[23] 程燕林. 中国部分Botryosphaeriaceae真菌的系统发育及模式菌Botryosphaeria dothidea的遗传多样性研究[D]. 北京: 中国林业科学研究院, 2012.
[24] 马翔, 迟文娟. 木棉干腐病病原鉴定及发病因素[J]. 中国森林病虫, 2014, 33(6): 1-4.
[25] 王璠, 黄俊斌, 李国怀, 等. 葡萄座腔菌属Botryosphaeria引起的果树病害及研究进展[J]. 植物保护, 2013, 39(6): 7-13.
[26] 周如军, 孙建潮, 薛彩云, 等. 花生褐斑病菌和网斑病菌混合侵染对侵染概率和潜育期的影响[J]. 中国农业科学, 2015, 48(21): 4264-4271.
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