Journal of Tea Science ›› 1998, Vol. 18 ›› Issue (02): 119-124.doi: 10.13305/j.cnki.jts.1998.02.007

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Hydroxyprol ine-rich Glycoprotein ( HRGP) Separation and Identification in Tea Leaves and Its Inducing by Rare Earths

The protein in tea polysaccha rides was separ ated and its components were identified. The results showed that there wa s hydroxy proline-rich gly coprotein (HRGP) in tea leaves and the hydorxy proline was in combination with tea polysaccharides. The content of hydroxy proline in HRGP purified by proteinase and Sevag method was over 30%, Unpurified HRGP about 8%-10%. HRGP could be dissolved in water and extracted by boiling water. There was little HRGP presented in tea residue. HRGP was related to plant hardiness. Spraying rare earths solution could induce tea plants to produce proline and promote it to be transformed into hydroxy proline whether tea plants were pruned or not. The conditions of separating and purifying glycoprotein, the existing pattern and formation way of hydroxy proline in tea plants and the mechanism o f hydroxyproline inducing by rare earths were also discussded in this paper.   

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