In this study, Pu-erh tea was extracted with acetone, water, chloroform, ethyl acetate, and n-butanol. then, the extraction was concentrated with rotary evaporation and dried with lyophilizer into chloroform layer, ethyl acetate layer, n-butanol layer and surplus water layer extracts, which were used to administer ICR obese mouse for analyzing the lipid-lowering and anti-obesity effects. The experimental results demonstrated that the pu-erh tea total extract, ethyl acetate extract and surplus water extract are all effective on lipid-lowering and anti-obesity of ICR obese mouse, and the effects of ethyl acetate extract and surplus water extract on inhibiting fat accumulation（inhibition ratio was 24.7% and 22.5%）was slightly less than those in the L-carnitine （38.8%）, but the effect on improving HDL-C level(61.7% and 73.4%) significantly superior to that in the L-carnitine (9.4%), the similar results were obtained in the mice liver, which suggest that the key lipid-lowering and anti-obesity function components were existed in the ethyl acetate layer and (or) surplus water layer extracts of pu-erh tea.