Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM

ZHANG Jianyong; CHEN Lin; CUI Hongchun; WANG Weiwei; XUE Jinjin; XIONG Chunhua; JIANG Heyuan

doi:10.13305/j.cnki.jts.20200117.002

Journal of Tea Science >

2020 , Vol. 40 >Issue 1: 51 - 62

DOI: https://doi.org/10.13305/j.cnki.jts.20200117.002

Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM

ZHANG Jianyong ,
CHEN Lin ,
CUI Hongchun ,
WANG Weiwei ,
XUE Jinjin ,
XIONG Chunhua ,
JIANG Heyuan

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1. School of Food Science and biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Tea Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received date: 2019-11-04

Revised date: 2019-12-22

Online published: 2020-02-04

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Abstract

The effect of chemical synthesis conditions on the yield of theasinensin A was studied. Key factors for chemical synthesis of theasinensin A (TSA) were determined by single factor test and Plackett-Burman Design (PBD). The response surface methodology (RSM) was employed to optimize the technical parameters for the chemical synthesis. The experiment results indicate that the effects of copper oxide content, methanol content and temperature on the yield were extremely significant. The main factors were methanol content, next copper oxide content, and then temperature. The optimal conditions were as follows: copper chloride content 43%, methanol content 26%, and temperature 15℃. Under these conditions, the yield of TSA was 59.12%, which was close to the predicted value of 59.34%. The combination of PBD and RSM to optimize the chemical synthesis of TSA achieved ideal result, which could provide reference and theoretical basis for the efficient chemical synthesis of other catechin oxidation polymers.

Key words： theasinensin A (TSA); EGCG; chemical synthesis; PBD; RSM; optimization

Cite this article

ZHANG Jianyong , CHEN Lin , CUI Hongchun , WANG Weiwei , XUE Jinjin , XIONG Chunhua , JIANG Heyuan . Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM[J]. Journal of Tea Science, 2020 , 40(1) : 51 -62 . DOI: 10.13305/j.cnki.jts.20200117.002

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