Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

QIU Shiting; HOU Xue; LEI Shaorong; HAN Mei; HE Guangyun; LI Ying; QIN Shudi

doi:10.13305/j.cnki.jts.2023.02.012

2023 , Vol. 43 >Issue 2: 216 - 226

DOI: https://doi.org/10.13305/j.cnki.jts.2023.02.012

Research Paper

Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

QIU Shiting ,
HOU Xue ,
LEI Shaorong ,
HAN Mei ,
HE Guangyun ,
LI Ying ,
QIN Shudi

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1. Institute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
2. Laboratory of Risk Assessment for Agricultural Product (Chengdu), Ministry of Agriculture and Rural Affairs, Chengdu 610066, China

Received date: 2023-01-04

Revised date: 2023-03-03

Online published: 2023-05-05

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Abstract

Nicotinamide ribosome (NR), nicotinamide mononucleotide (NMN), nicotinic acid (NA) and nicotinamide (NAM) are 4 precursor compounds of NAD⁺, which can be converted to NAD⁺ in vivo to work after oral administration. This study established a method for simultaneous determination of NAD⁺and its four precursors in tea by ultra-high performance liquid chromatography-tandem mass spectrometry. The tea samples were extracted by water, then diluted and directly analyzed by UPLC-MS/MS. Method validation shows this method has good linearity in their respective range with correlation coefficients (R²) higher than 0.99. The average recoveries ranged from 70.00 % to 120.00% with relative standard deviations (RSDs) of 2.09%-14.50%. The limits of quantitation (LOQs) were 0.10-0.50 μg·L^-1. The determination results of five target compounds in green tea, black tea and dark tea show that green tea and black tea were the good natural food sources of NR, NMN, NAD⁺. The results of principal component analysis show that the contents of five compounds could well distinguish black tea, green tea and dark tea. The cluster analysis shows the same type of tea from the same origin was of uneven quality, with high dispersion in samples, and it was difficult to identify each other.

Key words： tea; nicotinamideadenine dinucleotide (NAD⁺); nicotinamide nononucleotide (NMN); nicotinamide riboside (NR); UPLC-MS/MS

Cite this article

QIU Shiting , HOU Xue , LEI Shaorong , HAN Mei , HE Guangyun , LI Ying , QIN Shudi . Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Tea Science, 2023 , 43(2) : 216 -226 . DOI: 10.13305/j.cnki.jts.2023.02.012

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