Welcome to Journal of Tea Science,Today is
Research Paper

Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

  • QIU Shiting ,
  • HOU Xue ,
  • LEI Shaorong ,
  • HAN Mei ,
  • HE Guangyun ,
  • LI Ying ,
  • QIN Shudi
Expand
  • 1. Institute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
    2. Laboratory of Risk Assessment for Agricultural Product (Chengdu), Ministry of Agriculture and Rural Affairs, Chengdu 610066, China

Received date: 2023-01-04

  Revised date: 2023-03-03

  Online published: 2023-05-05

Abstract

Nicotinamide ribosome (NR), nicotinamide mononucleotide (NMN), nicotinic acid (NA) and nicotinamide (NAM) are 4 precursor compounds of NAD+, which can be converted to NAD+ in vivo to work after oral administration. This study established a method for simultaneous determination of NAD+ and its four precursors in tea by ultra-high performance liquid chromatography-tandem mass spectrometry. The tea samples were extracted by water, then diluted and directly analyzed by UPLC-MS/MS. Method validation shows this method has good linearity in their respective range with correlation coefficients (R2) higher than 0.99. The average recoveries ranged from 70.00 % to 120.00% with relative standard deviations (RSDs) of 2.09%-14.50%. The limits of quantitation (LOQs) were 0.10-0.50 μg·L-1. The determination results of five target compounds in green tea, black tea and dark tea show that green tea and black tea were the good natural food sources of NR, NMN, NAD+. The results of principal component analysis show that the contents of five compounds could well distinguish black tea, green tea and dark tea. The cluster analysis shows the same type of tea from the same origin was of uneven quality, with high dispersion in samples, and it was difficult to identify each other.

Cite this article

QIU Shiting , HOU Xue , LEI Shaorong , HAN Mei , HE Guangyun , LI Ying , QIN Shudi . Simultaneous Determination of Nicotinamide Adenine Dinucleotide and Its Four Precursors in Tea by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Tea Science, 2023 , 43(2) : 216 -226 . DOI: 10.13305/j.cnki.jts.2023.02.012

References

[1] Grant R, Berg J, Mestayer R, et al.A pilot study investigating changes in the human plasma and urine NAD+ metabolome during a 6 hour intravenous infusion of NAD+[J]. Frontiers in Aging Neuroscience, 2019, 11: 257-267.
[2] Zamporlini F, Ruggieri S, Mazzola F, et al.Novel assay for simultaneous measurement of pyridine mononucleotides synthesizing activities allows dissection of the NAD+ biosynthetic machinery in mammalian cells[J]. FEBS Journal, 2014, 281: 5104-5119.
[3] Zhu X H, Lu M, Lee B Y, et al.In vivo NAD assay reveals the intracellular NAD contents and redox state in healthy human brain and their age dependences[J]. PNAS, 2015, 112(9): 2876-2881.
[4] Massudi H, Grant R, Braidy N, et al.Age-associated changes in oxidative stress and NAD+ metabolism in human tissue[J]. Plos One, 2012, 7(7): e42357. doi: 10.1371/journal.pone.0042357.
[5] Hong W, Mo F, Zhang Z, et al.Nicotinamide mononucleotide: a promising molecule for therapy of diverse diseases by targeting NAD+ metabolism[J]. Frontiers in Cell and Developmental Biology, 2020, 8: 246. doi: 10.3389/fcell.2020.00246.
[6] Lautrup S, Sinclair D A, Mattson M P, et al.NAD+ in brain aging and neurodegenerative disorders[J]. Cell Metabolism, 2019, 30(4): 630-655.
[7] 刘晓谦, 杨红, 赵靖源, 等. UPLC-MS/MS测定铁皮石斛及其同属近源石斛品种中烟酰胺单核苷酸和烟酰胺腺嘌呤二核苷酸含量[J]. 中国中药杂志, 2021, 46(16): 4034-4039.
Liu X Q, Yang H, Zhao J Y, et al.Determination of β-nicotinamide mononucleotide and nicotinamide adenine dinucleotide in Dendrobium officinale and congeneric species by UPLC-MS/MS[J]. China Journal of Chinese Materia Medica, 2021, 46(16): 4034-4039.
[8] Ummarino S, Mozzon M, Zamporlini F, et al.Simultaneous quantitation of nicotinamide riboside, nicotinamide mononucleotide and nicotinamide adenine dinucleotide in milk by a novel enzyme-coupled assay[J]. Food Chemistry, 2017, 221(15): 161-168.
[9] Shi W, Hegeman M A, Dartel D, et al.Effects of a wide range of dietary nicotinamide riboside (NR) concentrations on metabolic flexibility and white adipose tissue (WAT) of mice fed a mildly obesogenic diet[J]. Molecular Nutrition & Food Research, 2017, 61(8): 1600878. doi: 10.1002/mnfr.201600878.
[10] Bogan K L, Brenner C.Nicotinic acid, nicotinamide, and nicotinamide riboside: a molecular evaluation of NAD+ precursor vitamins in human nutrition[J]. Annual Review of Nutrition, 2008, 28(1): 115-130.
[11] Bieganowski P, Brenner C.Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans[J]. Cell, 2004, 117(4): 495-502.
[12] Yoshino J, Mills K F, Yoon M J, et al.Nicotinamide mononucleotide, a key NAD+ intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice[J]. Cell Metabolism, 2011, 14(4): 528-536.
[13] Wu K, Li J Q, Zhou X H, et al.NADH and NRH as potential dietary supplements or pharmacological agents for early liver injury caused by acute alcohol exposure[J]. Journal of Functional Foods, 2021, 87: 104852. doi: 10.1016/j.jff.2021.104852.
[14] Mills K, Yoshida S, Stein L, et al.Long-term administration of nicotinamide mononucleotide mitigates age-associated physiological decline in mice[J]. Cell Metabolism, 2016, 24(6): 795-806.
[15] Zhang H B, Ryu D, Wu Y B, et al.NAD+ repletion improves mitochondrial and stem cell function and enhances life span in mice[J]. Science, 2016, 352(6292): 1436-1443.
[16] Cantó C, Menzies K, Auwerx J.NAD+ metabolism and the control of energy homeostasis: a balancing act between mitochondria and the nucleus[J]. Cell Metabolism, 2015, 22(1): 31-53.
[17] Yoshino J, Baur J A, Imai S I.NAD+ intermediates: the biology and therapeutic potential of NMN and NR[J]. Cell Metabolism, 2018, 27(3): 513-528.
[18] Fang E F, Lautrup S, Hou Y, et al.NAD+ in aging: molecular mechanisms and translational implications[J]. Trends in Molecular Medicine, 2017, 23(10): 899-916.
[19] 孙先枝, 刘小杰, 张芬, 等. 烟酰胺单核苷酸的生理功能及其在药品和食品中的应用[J]. 中国食品添加剂, 2022, 33(11): 246-251.
Sun X Z, Liu X J, Zhang F, et al.Nicotinamide mononucleotides physiological functions and its applications in medicine and food industry[J]. China Food Additives, 2022, 33(11): 246-251.
[20] 刘小芳, 蒋永毅, 王超, 等. 高效液相色谱-串联质谱法测定食品原料中烟酰胺单核苷酸的含量[J]. 食品科技, 2021, 46(8): 251-256, 262.
Liu X F, Jiang Y Y, Wang C, et al.Determination of nicotinamide mononucleotide in the natural food materials by high performance liquid chromatography-mass spectrometry[J]. Food Science and Technology, 2021, 46(8): 251-256, 262.
[21] 杨月欣. 中国食物成分表标准版:第一册[M]. 第6版. 北京: 北京大学医学出版社, 2018.
Yang Y X.China food composition tables standard edition: Volume 1 [M]. Version 6. Beijing: Peking University Medical Press, 2018.
[22] 戴申, 鹿颜, 余鹏辉, 等. 茶叶预防衰老及衰老相关疾病研究进展[J]. 茶叶科学, 2019, 39(1): 23-33.
Dai S, Lu Y, Yu P H, et al.Research progress of the preventing effects of tea on aging and aged-related pathologies[J]. Journal of Tea Science, 2019, 39(1): 23-33.
[23] 申雯, 黄建安, 李勤, 等. 茶叶主要活性成分的保健功能与作用机制研究进展[J]. 茶叶通讯, 2016, 43(1): 8-13, 65.
Shen W, Huang J A, Li Q, et al.The research progress of health care function and mechanism of the active ingredients in tea[J]. Journal of Communication, 2016, 43(1): 8-13, 65.
[24] Yaku K, Okabe K, Nakagawa T.Simultaneous measurement of NAD metabolome in aged mice tissue using liquid chromatography tandem-mass spectrometry[J]. Biomedical Chromatography, 2018, 32(6): e4205. doi: 10.1002/bmc.4205.
[25] Yoshino J, Imai S I.Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography[J]. Methods in Molecular Biology, 2013, 1077: 203-215.
[26] Stocchi V, Cucchiarini L, Magnani M, et al.Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells[J]. Analytical Biochemistry, 1985, 146(1): 118-124.
[27] 李东芹. 液质联用法测定蔬菜和水果中的烟酰胺单核苷酸[J]. 实验技术与管理, 2019, 36(9): 57-59, 72.
Li D Q.Determination of nicotinamide mononucleotides in vegetables and fruits by liquid chromatography-mass spectrometry[J]. Experimental Technology and Management, 2019, 36(9): 57-59, 72.
[28] 胡高华, 曹建荣, 杨蕾文宣, 等. 基于壳聚糖/氧化石墨烯/硅藻土固相萃取-液相色谱串联质谱测定茶叶中多种农药残留[J]. 茶叶科学, 2022, 42(2): 249-262.
Hu G H, Cao J H, Yanlei W X, et al.Determination of multi-pesticide residues in tea based on the combination of CS/GO/DM SPE column and UPLC-MS/MS[J]. Journal of Tea Science, 2022, 42(2): 249-262.
[29] Ferrer C, Lozano A, Agüera A, et al.Overcoming matrix effects using the dilution approach in multiresidue methods for fruits and vegetables[J]. Journal of Chromatography A, 2011, 1218(42): 7634-7639.
[30] 柯朝甫, 张涛, 武晓岩, 等. 代谢组学数据分析的统计学方法[J]. 中国卫生统计, 2014, 31(2): 357-359.
Ke C F, Zhang T, Wu X Y, et al.Statistical methods for metabolomics data analysis[J]. Chinese Journal of Health Statistics, 2014, 31(2): 357-359.
Outlines

/