Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress

LI Jiasi; LIU Yingqing; ZHANG Yongheng; ZHANG Ying'ao; XIAO Yezi; LIU Lu; YU Youben

doi:10.13305/j.cnki.jts.2023.03.007

Journal of Tea Science >

2023 , Vol. 43 >Issue 3: 325 - 334

DOI: https://doi.org/10.13305/j.cnki.jts.2023.03.007

Research Paper

Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress

LI Jiasi ,
LIU Yingqing ,
ZHANG Yongheng ,
ZHANG Ying'ao ,
XIAO Yezi ,
LIU Lu ,
YU Youben

Expand

College of Horticulture, Northwest A&F University, Yangling 712100, China

Received date: 2023-02-12

Revised date: 2023-04-03

Online published: 2023-06-29

Fold

Abstract

Nine cis epoxide carotenoid dioxygenase (NCED) is a key rate-limiting enzyme in abscisic acid (ABA) synthesis and widely involved in plant growth and development as well as abiotic stress response. CsNCED2 is involved in the response to drought and salt stress in tea plants, while the transcriptional regulation mechanism involved is still unclear. In this study, two transcription factors, CsDof5.4 and CsERF38, which binded to the CsNCED2 promoter were identified by yeast single hybrid (Y1H) library screening. Subcellular localization, yeast self-activation and luciferase (LUC) assay show that they were located in the cell nucleus and could activate the expression of CsNCED2. RT-qPCR results show that the expressions of CsERF38 and CsDof5.4 were highly correlated with CsNCED2 under salt stress. While under drought stress, only the expression of CsERF38was highly correlated with that of CsNCED2. In this study, two transcription factors (CsDof5.4 and CsERF38) binding to the CsNCED2 promoter were identified. Both drought and salt stresses could induce the expression of CsNCED2, thus participate in the abiotic stress response in tea plants.

Key words： tea plant; CsNCED2; transcriptional regulation; expression analysis

Cite this article

LI Jiasi , LIU Yingqing , ZHANG Yongheng , ZHANG Ying'ao , XIAO Yezi , LIU Lu , YU Youben . Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress[J]. Journal of Tea Science, 2023 , 43(3) : 325 -334 . DOI: 10.13305/j.cnki.jts.2023.03.007

References

[1] Leung J, Giraudat J.Abscisic acid signal transduction[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1998, 49: 199-222.
[2] Chen K, Li G J, Bressan R A, et al.Abscisic acid dynamics, signaling, and functions in plants[J]. Journal of Integrative Plant Biology, 2020, 62(1): 25-54.
[3] Shu K, Chen Q, Wu Y R, et al.ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein Levels[J]. The Plant Journal, 2016, 85(3): 348-361.
[4] Shu K, Chen Q, Wu Y, et al.ABSCISIC ACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LOCUS C transcription[J]. Journal of Experimental Botany, 2015, 67(1): 195-205.
[5] 陈唯, 曾晓贤, 谢楚萍, 等. 植物内源ABA水平的动态调控机制[J]. 植物学报, 2019, 54(6): 677-687.
Chen W, Zeng X X, Xie C P, et al.The dynamic regulation mechanism of the endogenous ABA in plant[J]. Chinese Bulletin of Botany, 2019, 54(6): 677-687.
[6] Sauter A, Davies W J, Hartung W.The long-distance abscisic acid signal in the droughted plant: the fate of the hormone on its way from root to shoot[J]. Journal of Experimental Botany, 2001, 52(363): 1991-1997.
[7] Ding F, Wang X Z, Li Z Y, et al.Jasmonate positively regulates cold tolerance by promoting ABA biosynthesis in tomato[J]. Plants, 2022, 12(1): 60. doi: 10.3390/plants12010060.
[8] Huang Y Y, Zhou J H, Li Y X, et al.Salt stress promotes abscisic acid accumulation to affect cell proliferation and expansion of primary roots in rice[J]. International Journal of Molecular Sciences, 2021, 22(19): 10892. doi: 10.3390/ijms221910892.
[9] Peng Z, Hu Y, Zhang J L, et al.Xanthomonas translucens commandeers the host Rate-limiting step in ABA biosynthesis for disease susceptibility[J]. PNAS, 2019, 116(42): 20938-20946.
[10] Mittler R, Blumwald E.The roles of ROS and ABA in systemic acquired acclimation[J]. The Plant Cell, 2015, 27(1): 64-70.
[11] Lefebvre V, North H, Frey A, et al.Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy[J]. The Plant Journal, 2006, 45(3): 309-319.
[12] Pei X X, Wang X Y, Fu G Y, et al.Identification and functional analysis of 9-cis-epoxy carotenoid dioxygenase (NCED) homologs in G. Hirsutum[J]. International Journal of Biological Macromolecules, 2021, 182: 298-310.
[13] Huang Y, Jiao Y, Xie N K, et al.OsNCED5, a 9-cis-epoxycarotenoid dioxygenase gene, regulates salt and water stress tolerance and leaf senescence in rice[J]. Plant Science, 2019, 287: 110188. doi: 10.1016/j.plantsci.2019.110188.
[14] Jiang Y J, Liang G, Yu D Q.Activated expression of WRKY57 confers drought tolerance in Arabidopsis[J]. Molecular Plant, 2012, 5(6): 1375-1388.
[15] Huang S Z, Ma Z M, Hu L J, et al.Involvement of rice transcription factor OsERF19 in response to ABA and salt stress responses[J]. Plant Physiology and Biochemistry, 2021, 167: 22-30.
[16] An S M, Liu Y, Sang K Q, et al.Brassinosteroid signaling positively regulates abscisic acid biosynthesis in response to chilling stress in tomato[J]. Journal of Integrative Plant Biology, 2023, 65(1): 10-24.
[17] 丁杰荣, 张静, 江立群, 等. OsWRKY67负向调控水稻耐旱性的功能分析[J]. 分子植物育种, 2023, 21(10): 3272-3281.
Ding J R, Zhang J, Jiang L Q, et al.Function analysis of OsWRKY67 negatively regulating drought-tolerance in rice[J]. Molecular Plant Breeding, 2023, 21(10): 3272-3281.
[18] 倪子鑫, 武清扬, 杨云, 等. 茶树CsCCD基因家族全基因组鉴定及乌龙茶LED补光晾青下表达分析[J]. 生物工程学报, 2022, 38(1): 359-373.
Ni Z X, Wu Q Y, Yang Y, et al.Genome-wide identification of CsCCD gene family in tea plant (Camellia sinensis) and expression analysis of the oolong tea processing with supplementary LED light[J]. Chinese Journal of Biological Engineering, 2022, 38(1): 359-373.
[19] Cho J-Y, Mizutani M, Shimizu B, et al.Chemical profiling and gene expression profiling during the manufacturing process of taiwan oolong tea “oriental beauty”[J]. Bioscience, Biotechnology, and Biochemistry, 2007, 71(6): 1476-1486.
[20] 王赞, 陈丹, 岳川, 等. 茶树CsNCED2基因的克隆和表达分析[J]. 西北植物学报, 2018, 38(6): 994-1002.
Wang Z, Chen D, Yue C, et al.Cloning and expression analysis of CsNCED2 gene in tea plant (Camellia sinensis)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(6): 994-1002.
[21] Liu S C, Jin J Q, Ma J Q, et al.Transcriptomic analysis of tea plant responding to drought stress and recovery[J]. Plos One, 2016, 11(1): e0147306. doi: 10.1371/journal.pone.0147306.
[22] Zhang Y H, Xiao Y Z, Zhang Y G, et al.Accumulation of galactinol and ABA is involved in exogenous EBR-induced drought tolerance in tea plants[J]. Journal of Agricultural and Food Chemistry, 2022, 70(41): 13391-13403.
[23] Gao M J, Yin X, Yang W B, et al.GDSL lipases modulate immunity through lipid homeostasis in rice[J]. PLOS Pathogens, 2017, 13(11): e1006724. doi: 10.1371/journal.ppat.1006724.
[24] Patankar H V, Al-Harrasi I, Al-Yahyai R, et al.Functional characterization of date palm aquaporin gene PdPIP1;2 confers drought and salinity tolerance to yeast and arabidopsis[J]. Genes, 2019, 10(5): 390. doi: 10.3390/genes10050390.
[25] Yoon J S, Flores P C, Seo Y W.Overexpression of BdASR2 plays a positive role in response to salt tolerance in Brachypodium distachyon[J]. Journal of Plant Biology, 2021, 65: 111-119.
[26] Beisson F, Li Y H, Bonaventure G, et al.The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis[J]. The Plant Cell, 2007, 19(1): 351-368.
[27] 黄桂媛, 张瑛, 林玲, 等. 酵母单杂交文库构建及巨峰葡萄VvFT基因启动子上游调控因子筛选[J]. 南方农业学报, 2020, 51(12): 2875-2883.
Huang G Y, Zhang Y, Lin L, et al.Yeast one-hybrid library construction and screening of upstream regulators of VvFT promoter in kyoho grape[J]. Journal of Southern Agriculture, 2020, 51(12): 2875-2883.
[28] 许奕, 李羽佳, 魏卿, 等. 香蕉MaAQP1启动子诱饵载体及干旱胁迫酵母单杂交cDNA文库的构建[J]. 福建农业学报, 2020, 35(10): 1078-1085.
Xu Y, Li Y J, Wei Q, et al.Constructions of banana MaAQP1 bait vector and drought-resistance cDNA library[J]. Fujian Journal of Agricultural Sciences, 2020, 35(10): 1078-1085.
[29] 郇蕾, 王旭旭, 陈修淼, 等. 桃ABA信号关键基因PpABI5酵母单杂交文库构建及其上游转录因子的筛选[J]. 植物生理学报, 2017, 53(7): 1259-1266.
Huan L, Wang X X, Chen X M, et al.Constructing yeast one-hybrid library and screening the potential regulator of PpABI5 in peach (Prunus persica)[J]. Plant Physiology Journal, 2017, 53(7): 1259-1266.
[30] Lee S U, Mun B G, Bae E K, et al.Drought stress-mediated transcriptome profile reveals NCED as a key player modulating drought tolerance in Populus davidiana[J]. Frontiers in Plant Science, 2021, 12: 755539. doi: 10.3389/fpls.2021.755539.
[31] Estrada-Melo A C, Ma Co, Reid M S, et al. Overexpression of an ABA biosynthesis gene using a stress-inducible promoter enhances drought resistance in Petunia[J]. Horticulture Research, 2015, 2(1): 15013. doi: 10.1038/hortres.2015.13.
[32] Wan S Q, Wang W D, Zhou T S, et al.Transcriptomic analysis reveals the molecular mechanisms of Camellia sinensis in response to salt stress[J]. Plant Growth Regulation, 2018, 84(3): 481-492.
[33] Ni Z Q, Jin J, Ye Y, et al.Integrative transcriptomic and phytohormonal analyses provide insights into the cold injury recovery mechanisms of tea leaves[J]. Plants, 2022, 11(20): 2751. doi: 10.3390/plants11202751.
[34] Shang X G, Yu Y J, Zhu L J, et al.A cotton NAC transcription factor GhirNAC2 plays positive roles in drought tolerance via regulating ABA biosynthesis[J]. Plant Science, 2020, 296: 110498. doi: 10.1016/j.plantsci.2020.110498.
[35] Ma H Z, Liu C, Li Z X, et al.ZmbZIP4 contributes to stress resistance in maize by regulating ABA synthesis and root development[J]. Plant Physiology, 2018, 178(2): 753-770.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References