Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1

WANG Jinbo; XIE Siyi; DOU Xiangya; SHEN Xiaohua; TIAN Na; LIU Shuoqian

doi:10.13305/j.cnki.jts.2025.02.005

Journal of Tea Science >

2025 , Vol. 45 >Issue 2: 191 - 200

DOI: https://doi.org/10.13305/j.cnki.jts.2025.02.005

Research Paper

Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1

WANG Jinbo ,
XIE Siyi ,
DOU Xiangya ,
SHEN Xiaohua ,
TIAN Na ,
LIU Shuoqian

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1. Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Ministry of Education, Changsha 410128, China;
2. Department of Tea Science, College of Horticulture, Hunan Agricultural University, Changsha 410128, China

Received date: 2024-11-07

Revised date: 2024-12-27

Online published: 2025-04-30

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Abstract

The Patellin (PATL) gene family is essential for plant growth, development and environmental adaptation. This study systematically identified and analyzed the CsPATL gene family in tea plants (Camellia sinensis). Five members of the CsPATL family were identified using a variety of bioinformatics techniques, and the physicochemical characteristics of their protein sequences were analyzed. The results show that the five CsPATL genes encode 232~585 amino acids, their molecular weights are 26.31~64.69 kDa, and their theoretical isoelectric points are 4.65~9.35. The chromosomal localization of these genes and the cis-elements in their promoter sequences were also analyzed in detail, and it was found that these genes were unevenly distributed on four chromosomes, and were mainly involved in phytohormone response, abiotic stress response, and light response, with light-responsive elements occupying a significant proportion in particular. Y1H, EMSA, and dual-luciferase assays confirm that Cshdz7 can directly bind to the CsPATL1 promoter and promote CsPATL1 gene expression. These findings provided a new perspective on the role of the CsPATL family genes in plant developmental regulation of tea plant development.

Key words： Camellia sinensis; gene family; CsPATL1; promoter; transcriptional regulation

Cite this article

WANG Jinbo , XIE Siyi , DOU Xiangya , SHEN Xiaohua , TIAN Na , LIU Shuoqian . Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1[J]. Journal of Tea Science, 2025 , 45(2) : 191 -200 . DOI: 10.13305/j.cnki.jts.2025.02.005

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