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Research Paper

Study on the Genetic Diversity of 78 Tea Germplasm Resources in Hunan Based on Agronomic Traits and SNP Molecular Markers

  • GUO Jialu ,
  • QU Furong ,
  • CAI Tianchen ,
  • ZHAO Yang ,
  • YANG Peidi ,
  • LIU Yong ,
  • ZHOU Yuebin ,
  • LIU Zhen
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  • 1. Hunan Agricultural University, Changsha 410125, China;
    2. Tea Research Institute, Hunan Academy of Agricultural Sciences, Hunan Branch of National Tea Improvement Center, Changsha 410125, China;
    3. Huangpu Innovation Research Institute, Hunan Agricultural University, Guangzhou 510700, China

Received date: 2024-11-07

  Revised date: 2025-01-20

  Online published: 2025-04-30

Abstract

To clarify the genetic diversity of tea germplasm resources in Hunan Province, explore the genetic structure of germplasm resources for accurate identification and evaluation, genotyping by targeted sequencing (GBTS) was used to genotype and analyze the genetic diversity of 76 252 SNP loci in 78 tea germplasm resources in Hunan Province. A total of 30 agronomic traits and 15 biochemical components of this batch of resources were accurately identified. The results show that there was abundant genetic variation among 78 tea germplasm resources in Hunan Province, with a genetic diversity index of 0.07-2.08 for phenotypic traits and a coefficient of variation of 2.26%-47.50%. The smallest was the number of calyxs and the largest was the depth of leaf serration. The genetic diversity index of the biochemical components was 1.36-2.09, and the coefficient of variation was 5.90%-118.49%, with the smallest being water extract and the largest being gallic acid. When the Euclidean distance was 20, the 78 tea germplasm resources could be divided into five groups, and there were significant differences in leaf length, leaf size, leaf base shape, ratio of polyphenols/amino acids, GA, THEO, GC, EGC, EC, EGCG, GCG and ECG in groups Ⅰ and Ⅲ. Based on the genotype detection results of 78 resources, a phylogenetic tree was constructed, and 78 resources could be divided into three groups. At the same time, this study identified 23 tea resources with specific phenotypic traits, high levels of functional components such as amino acids, theobromine and caffeine. The results of this study could provide a basis for the protection and utilization of tea germplasm resources in Hunan.

Cite this article

GUO Jialu , QU Furong , CAI Tianchen , ZHAO Yang , YANG Peidi , LIU Yong , ZHOU Yuebin , LIU Zhen . Study on the Genetic Diversity of 78 Tea Germplasm Resources in Hunan Based on Agronomic Traits and SNP Molecular Markers[J]. Journal of Tea Science, 2025 , 45(2) : 219 -233 . DOI: 10.13305/j.cnki.jts.2025.02.002

References

[1] 李瑞. 陕南茶区茶树种质资源的鉴定与评价[D]. 杨凌: 西北农林科技大学, 2011.
Li R.Identification and evaluation of tea germplasm resources in southern Shaanxi[D]. Yangling: Northwest A & F University, 2011.
[2] 杨凤, 刘霞, 尹杰, 等. 贵州野生茶树种质资源的主要生化成分及抗旱性评价[J]. 西南农业学报, 2018, 31(6): 1122-1127.
Yang F, Liu X, Yin J, et al.Major biochemical components and drought resistance of wild tea germplasm resources in Guizhou[J]. Southwest China Journal of Agricultural Sciences, 2018, 31(6): 1122-1127.
[3] 江昌俊. 茶树育种学[M]. 北京: 中国农业出版社, 2021.
Jiang C J.Tea breeding[M]. Beijing: China Agriculture Press, 2021.
[4] 田甜, 韦锦坚, 韦持章, 等. 35份茶树种质资源农艺性状及生化成分多样性分析[J]. 西北农业学报, 2017, 26(5): 797-804.
Tian T, Wei J J, Wei C Z, et al.Diversity analysis of agronomic traits and biochemical components of 35 tea germplasm resources[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2017, 26(5): 797-804.
[5] 杨春, 乔大河, 郭燕, 等. 115份贵州茶树资源氨基酸和茶氨酸分析与特异资源筛选[J]. 浙江农业学报, 2022, 34(7): 1351-1360.
Yang C, Qiao D H, Guo Y, et al.Analysis into amino acids and theanine contents of 115 tea germplasms and special germ-plasm resource screening in Guizhou, China[J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1351-1360.
[6] 尚卫琼, 段志芬, 邓少春, 等. 云南地方茶树种质资源特异性评价[J]. 中国农学通报, 2022, 38(34): 76-81.
Shang W Q, Duan Z F, Deng S C, et al.Specificity evaluation of local tea germplasm gesources in Yunnan[J]. Chinese Agricultural Science Bulletin, 2022, 38(34): 76-81.
[7] 陈潇敏, 金珊, 吴文晞, 等. 高表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯茶树种质资源的筛选与评价[J]. 福建农业学报, 2022, 37(4): 467-475.
Chen X M, Jin S, Wu W X, et al.Screening and evaluation of tea germplasms containing high-EGCG3"Me[J]. Fujian Journal of Agricultural Sciences, 2022, 37(4): 467-475.
[8] 翟秀明, 李解, 唐敏, 等. 重庆30份茶树种质资源农艺性状与生化成分多样性[J]. 浙江农业学报, 2021, 33(7): 1244-1255.
Zhai X M, Li J, Tang M, et al.Diversity analysis of 30 tea (Camelia sinensis) germplasm resources in Chongqing based on a gronomic traits and biochemical components[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1244-1255.
[9] Li M, Meegahakumbura M K, Wambulwa M C, et al.Genetic analyses of ancient tea trees provide insights into the breeding history and dissemination of Chinese Assam tea (Camellia sinensis var. assamica)[J]. Plant Diversity, 2024, 46(2): 229-237.
[10] Guo R, Xia X B, Chen J, et al.Genetic relationship analysis and molecular fingerprint identification of the tea germplasms from Guangxi Province, China[J]. Breeding Science, 2021, 71(5): 584-593.
[11] Zhang J, Yang J J, Zhang L, et al.A new SNP genotyping technology target SNP-seq and its application in genetic analysis of cucumber varieties[J]. Scientific Reports, 2020, 10(1): 5623. doi: 10.1038/s41598-020-62518-6.
[12] 徐云碧, 杨泉女, 郑洪建, 等. 靶向测序基因型检测(GBTS)技术及其应用[J]. 中国农业科学, 2020, 53(15): 2983-3004.
Xu Y B, Yang Q N, Zheng H J, et al.Genotyping by target sequencing(GBTS) and its applications[J]. Scientia Agricultura Sinica, 2020, 53(15): 2983-3004.
[13] 李军玲, 刘燕清, 崔中秋, 等. 水稻重要农艺性状控制基因GBTS液相芯片开发及应用[J/OL]. 分子植物育种, 2023: 1-19[2024-11-07]. http://kns.cnki.net/kcms/detail/46.
1068.S.20231115.1312.008.html.Li J L, Liu Y Q, Cui Z Q, et al. Development and application of GBTS liquid chip for genes controlling important agronomic traits in rice[J]. Molecular Plant Breeding, 2023: 1-19[2024-11-07]. . Development and application of GBTS liquid chip for genes controlling important agronomic traits in rice[J]. Molecular Plant Breeding, 2023: 1-19[2024-11-07]. http://kns.cnki.net/kcms/detail/46.1068.S.20231115.1312.008.html.
[14] Li Z, Jia Z, Li J, et al.Development of a 45K pepper GBTS liquid-phase gene chip and its application in genome-wide association studies[J]. Frontiers in Plant Science, 2024, 15: 1405190. doi: 10.3389/fpls.2024.1405190.
[15] Yu D, Song L, Gu W, et al.Genome-wide comparative analysis of genetic diversity of regular and specialty maize inbred lines through genotyping by target sequencing (GBTS)[J]. Plant Molecular Biology Reporter, 2021, 40(2): 1-11.
[16] Yang Q, Zhang J N, Shi X L, et al.Development of SNP marker panels for genotyping by target sequencing (GBTS) and its application in soybean[J]. Molecular Breeding, 2023, 43(4): 26. doi: 10.1007/s11032-023-01372-6.
[17] 刘振, 赵洋, 杨培迪, 等. 湖南省茶树种质资源现状及研究进展[J]. 茶叶通讯, 2011, 38(3): 7-10.
Liu Z, Zhao Y, Yang P D, et al.The status and research progress of Hunan tea germplasm[J]. Journal of Tea Communication, 2011, 38(3): 7-10.
[18] 陈亮, 杨亚军, 虞富莲, 等. 茶树种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2005.
Chen L, Yang Y J, Yu F L, et al.Specification and data standards for the description of tea germplasm resources[M]. Beijing: China Agriculture Press, 2005.
[19] 张海平, 房伟民, 陈发棣, 等. 部分睡莲属植物形态性状的多样性分析[J]. 南京农业大学学报, 2009, 32(4): 47-52.
Zhang H P, Fang W M, Chen F L, et al.Investigation on the morphological diversity of taxa in genus Nymphaea[J]. Journal of Nanjing Agricultural University, 2009, 32(4): 47-52.
[20] Kumar S, Stecher G, Li M, et al.MEGA X: molecular evolutionary genetics analysis across computing platforms[J]. Molecular Biology Evolution, 2018, 35(6): 1547-1549.
[21] 丁帅涛, 程晓梅, 张亚, 等. 基于表型性状和生化成分的陕西茶树种质资源遗传多样性研究[J]. 西北农业学报, 2019, 28(4): 607-619.
Ding S T, Cheng X M, Zhang Y, et al.Genetic diversity of tea germplasm resources in Shaanxi Province based on phenotypic traits and biochemical components[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(4): 607-619.
[22] 王启, 刘琨毅, 李若愚, 等. 10份古茶树资源的生化成分测定分析[J/OL]. 分子植物育种, 2024: 1-12[2024-11-07]. http://kns.cnki.net/kcms/detail/46.1068.S.20240704.1416.004.html.
Wang Q, Liu K Y, Li R Y, et al. Determination and analysis of biochemical components in 10 ancient tea Resources[J/OL]. Molecular Plant Breeding, 2024: 1-12[2024-11-07]. http://kns.cnki.net/kcms/detail/46.1068.S.20240704.1416.004.html.
[23] 李冉阳, 袁海波, 屈丽池, 等. 红茶贮藏过程中的品质变化机理及研究进展[J/OL]. 食品与发酵工业, 2024: 1-10[2024-09-09]. https://doi.org/10.13995/j.cnki.11-1802/ts.040208.
Li R Y, Yuan H B, Qu L C, et al. Mechanism and research progress of quality change of black tea during storage[J/OL]. Food and Fermentation Industries, 2024: 1-10[2024-09-09]. https://doi.org/10.13995/j.cnki.11-1802/ts.040208.
[24] 梅黎青, 徐雨琴, 刘庆辉, 等. 婺源茶树群体种的适制性研究及品质评价[J]. 食品研究与开发, 2024, 45(2): 51-57.
Mei L Q, Xu Y Q, Liu Q H, et al.Study on the processing suitability and quality evaluation of Wuyuan tea landraces[J]. Food Research and Development, 2024, 45(2): 51-57.
[25] 宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003.
Wan X C.Tea biochemistry[M]. Beijing: China Agriculture Press, 2003.
[26] 蔡利娅, 黎星辉, 胡茂丰, 等. 湖南地方茶树资源的研究[J]. 湖南农业大学学报, 1997(6): 48-52.
Cai L Y, Li X H, Hu M F, et al.Research on local tea resources in Hunan[J]. Journal of Hunan Agricultural University, 1997(6): 48-52.
[27] 陈潇敏, 章进汕, 金珊, 等. 福建大田茶树品种资源生化成分特征分析与评价[J]. 南方农业学报, 2022, 53(2): 381-390.
Chen X M, Zhang J S, Jin S, et al.Analysis of biochemical components of tea variety resources in Datian, Fujian[J]. Journal of Southern Agriculture, 2022, 53(2): 381-390.
[28] 王新超, 陈亮, 杨亚军. 广西茶树资源生化成分多样性分析[J]. 植物遗传资源学报, 2010, 11(3): 309-314.
Wang X C, Chen L, Yang Y J.Biochemical diversity analysis of tea germplasms in Guangxi[J]. Journal of Plant Genetic Resources, 2010, 11(3): 309-314.
[29] 陈正武, 陈娟, 龚雪, 等. 28份贵州茶树种质资源的生化成分多样性分析[J]. 西南农业学报, 2015, 28(4): 1517-1523.
Chen Z W, Chen J, Gong X, et al.Diversity analysis for biochemical components of 28 tea germplasm resources in Guizhou[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(4): 1517-1523.
[30] 潘宇婷. 河南地方茶树种质资源遗传多样性及亲缘关系分析[D]. 合肥: 安徽农业大学, 2019.
Pan Y T.Genetic diversity and relationship analysis of Henan local tea germplasm resources[D]. Hefei: Anhui Agricultural University, 2019.
[31] 董方, 李小飞, 沈思言, 等. 江西茶树资源的遗传多样性分析及优异种质筛选[J]. 江西农业大学学报, 2022, 44(6): 1466-1477.
Dong F, Li X F, Shen S Y, et al.Genetic diversity analysis and screening of excellent germplasm of tea plant resources in Jiangxi[J]. Acta Agriculturae Universitatis Jiangxiensis, 2022, 44(6): 1466-1477.
[32] 牛小军, 包兴伟, 郑旭霞, 等. 四川九龙古茶树种质资源农艺性状遗传多样性研究[J]. 植物遗传资源学报, 2023, 25(2): 249-258.
Niu X J, Bao X W, Zheng X X, et al.The phenotypic diversity analysis of ancient tea germplasm resources in Jiulong county, Sichuan province, China[J]. Journal of Plant Genetic Resources, 2023, 25(2): 249-258.
[33] 李力, 罗盛财, 王飞权, 等. 基于GBS-SNP的武夷茶树(Camellia sinensis, Synonym: Thea bohea L.)遗传分析及标记开发[J]. 茶叶科学, 2023, 43(3): 310-324.
Li L, Luo S C, Wang F Q, et al.Genetic analysis and marker development for Wuyi tea (Camellia sinensis, Synonym: Thea bohea L.) based on GBS-SNP[J]. Journal of Tea Science, 2023, 43(3): 310-324.
[34] 段继华, 雷雨, 康彦凯, 等. 21个茶树优良单株生化成分分析与特异资源筛选[J]. 茶叶通讯, 2020, 47(2): 215-220.
Duan J H, Lei Y, Kang Y K, et al.Biochemical component analysis and specific resource selection of 21 excellent individual tea plants[J]. Journal of Tea Communication, 2020, 47(2): 215-220.
[35] 林开勤, 李悦欣, 魏杰, 等. 基于SSR分子标记分析石阡茶树资源遗传多样性[J]. 西南农业学报, 2024, 37(7): 1435-1441.
Lin K Q, Li Y X, Wei J, et al.Genetic diversity of tea plant resources in Shiqian based on SSR molecular markers[J]. Southwest China Journal of Agricultural Sciences, 2024, 37(7): 1435-1441.
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