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Basic Information about the Journal
Journal title: Journal of Tea science
Inscription of journal title: ZHU De
Responsible Institution: China Association for Science and Technology
Sponsored by: China Tea Science Society
Tea Research Institute, Chinese Academy of Agricultural Science
Editing and Publishing: Editorial Office, Journal of Tea science
Start time: 1964
No. of issues: Bi-monthly
Two-Dimensional Code of Tea Science Website
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    Construction of AFLP Molecular Markers Linkage Map in Tea Plant
    HUANG Jian-an, LI Jia-xian, HUANG Yi-huan, LUO Jun-wu,GONG Zhi-hua, LIU Zhong-hua
    Journal of Tea Science    2005, 25 (1): 7-15.   DOI: 10.13305/j.cnki.jts.2005.01.002
    Abstract98)      PDF(pc) (962KB)(45)       Save
    Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map used a set of 69 F1 progenies of Qi Men No.4×Chao An Da Wu Ye as a mapping population. In this work, all together 1925 bands were obtained by 22 pairs of primers and 87.5 bands each primer pair on average, a total of 485 (25.19%) polymorphic AFLP markers were detected. The 356 (73.40%) of polymorphic markers with Mendelian segregation ratio (P=0.01) were obtained. 247(69.38%)of the 356 loci were 1:1 segregation, 109(30.62%)of the 356 loci were 3:1 segregation. Furthermore, the AFLP molecular markers linkage maps of Qi Men No.4 and Chao An Da Wu Ye were constructed respectively by using Mapmaker/Exp (version3.0). The map of female parent included 17 linkage groups and located 208 markers, covered total map length of 2457.7 cM, and average distance between markers was 11.9 cM. The map of male parent included 16 linkage groups and located 200 markers,covered total map length of 2545.3 cM, and average distance between markers was 12.8 cM.
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    Cited: Baidu(66)
    Effect of Tea Polysaccharides on Immune Functions and Antioxdative Activity in Broilers
    HU Zhong-ze, JIN Guang-ming, WANG Li-ke, YANG Jiu-feng
    Journal of Tea Science    2005, 25 (1): 61-64.   DOI: 10.13305/j.cnki.jts.2005.01.011
    Abstract60)      PDF(pc) (312KB)(23)       Save
    To study the effect of tea polysaccharide on immune function and antioxidative activity in broilers, 0%、0.2%、0.4% of tea polysaccharide were added into the drinking water for broilers respectively. Results showed that thymus index and serum IgG levels of broilers were increased obviously by TP(P<0.05), furthermore lymphocyte transformation rate, macrophage activity, percentage of T cell numbers Serum SOD, GSH-Px and CAT activities in broilers were increased obviously by TP at same time(P<0.05)but not to index of fabricius bursa of broilers. Serum MDA levels of broilers were declined obviously by TP(P<0.05).
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    Cited: Baidu(58)
    Effect of Dietary Tea Polyphenols on Performances, Lipid Metabolism and Egg Quality of Laying Hens
    LOU Hong-xing, LIN Zi, WANG You-ming, LU Fu-zhuang, TANG Jun-feng, YING Jun-feng, YANG Zhong-ming
    Journal of Tea Science    2004, 24 (2): 135-140.   DOI: 10.13305/j.cnki.jts.2004.02.012
    Abstract72)      PDF(pc) (283KB)(45)       Save
    There were two feeding trials in this research. 225 37-week-old Lohamann laying hens were randomly divided into five treatments for trial one,fed a basal diet supplemented with 0, 0.05, 0.1, 0.2, 0.4 percentage tea polyphenols(TP) for a period of 59 days, respectively.600 51-week-old Lohamann laying hens were randomly divided into four treatments for trial two, fed a basal diet added with 0, 0.025, 0.05, 0.1 percentage TP for a period of 8 weeks, respectively. Dietary effects of TP supplementation on performance, lipid metabolism and egg quality were investigated in laying hens. The results showed that: (1) Egg production and feed conversion efficiency were improved to a certain extent by supplementing TP, but they were not improved with the increasing levels of added TP. (2) The addition of 0.1% TP in diet increased egg yolk VE and VA concentration by 58.99% and 20.96%, respectively. And TP was tended to increase whole egg amino acid levels containing S. The supplementation of 0.025%—0.1% TP in diet of layers at later period decreased broken and soft-shelled egg rates by 31.59%—47.09%. (3) The addition of 0.4% TP in diet decreased total cholesterol (TCH), triglyceride(TG), LDL-C contents of layer serum with 46 week old by 19.65%, 19.99% and 7.69%, respectively, and increased HDL-C by 45.35%. No significant change of 0.025%—0.1% TP supplementation on serum lipid levels was found in layers with 59βw old.
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    Cited: Baidu(58)
    Elite Germplasm Evaluation and Genetic Stability of Tea Plants
    YANG Ya-jun, YU Fu-lian, CHEN Ling, ZENG Jian-ming, YANG Su-juan, LI Su-fang, SHU Ai-ming, ZHANG Zhi-fang, WANG Yu-shu, WANG Hai-si, WANG Ping-sheng, XU Mei, SONG Wei-xi, GUO Ji-chun, YANG Ru-xing, ZHANG Wen-jin, CHEN Zhi-hui
    Journal of Tea Science    2003, 23 (s1): 1-8.   DOI: 10.13305/j.cnki.jts.2003.s1.001
    Abstract79)      PDF(pc) (330KB)(34)       Save
    The agronomy, made tea quality, chemical components, cold resistance of 200 accessions of tea germplasms were investigated during 1996-2000. Among six accessions of elite tea germplasms, their made tea quality exceeded national clones, four with high polyphenol content (>38%), six with high caffeine content (>5.2%) and two with low caffeine content (<1%) germplasms were selected. Based on the studies of RAPD marker, isozyme and pollen morphology, the heredity of tea germplasms and their cutting offsprings was basically stable.
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    Cited: Baidu(58)
    Research Progress on Resistance Breeding of Tea Plant
    WANG Xin-chao,YANG Ya-jun
    Journal of Tea Science    2003, 23 (2): 94-98.   DOI: 10.13305/j.cnki.jts.2003.02.002
    Abstract78)      PDF(pc) (384KB)(52)       Save
    This paper summarized the progress of cold hardness resistance、drought resistance、resistance to disease and pest on tea breeding in china and abroad,and put forward some further research focal points on these aspects.
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    Cited: Baidu(56)
    Effect of Cold Acclimation and ABA on Cold Hardiness, Contents of Proline in Tea Plants
    YANG Ya-jun, ZHEN Lei-ying, WANG Xin-chao
    Journal of Tea Science    2004, 24 (3): 177-182.   DOI: 10.13305/j.cnki.jts.2004.03.006
    Abstract82)      PDF(pc) (158KB)(36)       Save
    Effect of cold acclimation and ABA on cold hardiness resistance, contents of proline in tea plants was studied. The results showed that through cold acclimation and ABA treatment, the cold hardiness of tea plants was changed greatly during the process of acclimation to deacclimation, showing a trend from low to high then to low again. And ascertain 7℃and 9℃are the crucial temperature to finish acclimation or start declimation respectively.On the other hand, it can be concluded that the rising degree of cold hardiness resistance was lower during artificial or ABA treatment than that of natural cold acclimation. At the same time, changes of Proline content weren’t regular,the relationship between proline content and cold hardiness resistance was insignificant. It implied that changes of proline content during cold acclimation were an integrative response in tea plant.
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    Cited: Baidu(53)
    Study on the Hypoglycemic Mechanism of Tea Polysaccharides and Tea Polyphenols
    DING Ren-feng, HE Pu-ming, JIE Guo-liang
    Journal of Tea Science    2005, 25 (3): 219-224.   DOI: 10.13305/j.cnki.jts.2005.03.011
    Abstract105)      PDF(pc) (399KB)(54)       Save
    The investigation on the hypoglycemic mechanism of tea polysaccharides and tea polyphenol in diabetic SD rats induced by alloxan was conducted. Methods: After feeding tea polysaccharides or polyphenols for 3 weeks, the changes of the level of blood glucose, insulin and the activities of amylase, sucrase and maltase in small intestine were inspected in diabetic rats. Results about that the suppressive effect of high level of blood glucose induced by alloxan was observed in the groups feeded with tea polysaccharides or tea polyphenols obviously in the experimental period. Compared with control group, the level of blood insulin in the group feeded with tea polysaccharides was increased significantly (P<0.05), the activity of sucrase and maltase were decreased significantly. Almost the same change without significance was observed in the group feeded with tea polyphenols while compared with the control group. Investigation indicated that the tea polysaccharides and tea polyphenols have remarkable suppressive hyperglycemic effect on diabetic rats induced by alloxan. These results suggested that functionary mechanisms of the suppressive hyperglycemic effect of tea polysaccharides may be induced by controlling the activities of sucrase and maltase.
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    Cited: Baidu(52)
    Analysis on Applicability of ISSR in Molecular Identification and Relationship Investigation of Tea Cultivars
    YAO Ming-zhe, HUANG Hai-tao, YU Ji-zhong, CHEN Liang
    Journal of Tea Science    2005, 25 (2): 153-157.   DOI: 10.13305/j.cnki.jts.2005.02.013
    Abstract90)      PDF(pc) (379KB)(52)       Save
    Inter-simple sequence repeat (ISSR) is a recently developed PCR-based molecular technology. It has been widely used in genetic diversity, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of crop species due to its simple, quick, efficient, high reproducibility and low expense. In this study, the applicability of ISSR on molecular identification and relationship analysis of tea cultivars were investigated. A total of 99 bands were amplified by eight ISSR primers in six tea cultivars and the average polymorphism was 79.6%. The six tea cultivars could be clearly distinguished by ISSR fingerprinting amplified with primers TRI22 and TRI30, and also by the presence or absence of specific ISSR markers and their combination. Six cultivars were divided into two groups by cluster analysis. Maolu, Cuifeng, Qingfeng, Yingshuang and Jinfeng were in one group and Longjing 43 in the other. The relationship of six tea cultivars were validated by cluster analysis based on ISSR. The results indicate that ISSR is a useful tool for molecular identification and relationship analysis of tea cultivars.
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    Cited: Baidu(48)
    Construction of Tender Shoots cDNA Library and Preliminary Analysis of Expressed Sequence Tags Sequencing of Tea Plant
    CHEN Liang, ZHAO Li-ping, GAO Qi-kang
    Journal of Tea Science    2004, 24 (1): 18-22.   DOI: 10.13305/j.cnki.jts.2004.01.003
    Abstract89)      PDF(pc) (423KB)(78)       Save
    The construction of the first cDNA library of tea plant [Camellia sinensis cv. Longjing 43] in China and the analysis of expressed sequence tags sequencing successful ratio were reported. Total RNA was isolated from tender tea shoots using TRIZOL single-step method, mRNA separated and then the double-strand cDNA amplified by LD-PCR. After size fractionation, the ds-cDNA was ligated to λTripEX2 and recombinant bacteriophages were packaged. The cDNA library was tittered and amplified using XL1-Blue as receptor bacterium. The titer of the original library was 6.8×105pfu/ml with a recombinant rate of 98.05% and 3.5×105 clones in total, the amplified titer was 7.2×109pfu/ml. PCR amplification suggested the inserted cDNA fragments ranged from 0.5 kb to 2 kb, mostly from 1.0 kb to 1.5 kb. The data indicate that the tea cDNA library has high titer, high recombinant percentage and large inserted fragments. Totally, 4320 clones were sequenced, 2963 useful sequences were obtained, corresponding to 68.5%. The first batch of 1687 valid tea plant ESTs were generated.
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    Cited: Baidu(48)
    Genetic Mapping of First Generation of Backcross in Tea by RAPD and ISSR Markers
    HUANG Fu-ping, LIANG Yue-rong, LU Jian-lian, CHEN Rong-bing
    Journal of Tea Science    2006, 26 (3): 171-176.   DOI: 10.13305/j.cnki.jts.2006.03.003
    Abstract65)      PDF(pc) (545KB)(39)       Save
    The segregation mode for 94 plants in first generation of back cross (BC1) of tea cultivar Fudingdabaicha were investigated using 14 RAPD primers and 20 ISSR primers. One hundred and seventy four segregation loci was identified. Among the 174 loci, 90 loci or 51.7% were segregated at the expected Mendelian ratio of 1:1, in which 63 belonged to ISSR loci and 27 belonged to RAPD loci, and 36 loci segregated in the ratios of 3:1 or 1:3. Sixty-two of the 126 loci with segregation ratios of 1:1, 3:1 and 1:3 were included in 7 linkage groups through linkage mapping by Mapmaker EXP 3.0 software and the other 64 loci were not included in the 7 linkage groups because of their far genetic distance. This map concluded 46 RAPD markers and 16 ISSR markers, covering distance of 1180.9βcM, with average distance of 20.1βcM. The linkage group LG4 covered the farthest genetic distance with 309.3βcM and group LG6 had the largest number of markers, with 18 loci and its average distance being 15.7βcM.
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    Cited: Baidu(47)
    The Improvement of Isolation and The Method of Two-Dimensional Electrophoresis of Protein from Tea Plant
    LIN Jin-ke, ZHENG Jin-gui, YUAN Ming, ZHANG Xue-qin, WANG Feng
    Journal of Tea Science    2003, 23 (1): 16-20.   DOI: 10.13305/j.cnki.jts.2003.01.004
    Abstract64)      PDF(pc) (699KB)(62)       Save
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    Cited: Baidu(46)
    Biochemical and Molecular Biological Basis on The Anticarcinogenic Activity of Tea Polyphenolic Compounds
    CHEN Zong-mao
    Journal of Tea Science    2003, 23 (2): 83-93.   DOI: 10.13305/j.cnki.jts.2003.02.001
    Abstract129)      PDF(pc) (495KB)(267)       Save
    The progress in the investigation of anticarcinogenic activity of tea polyphenols and its biochemical and molecular biological mechanisms in the world in recent 5 years were analyzed and reviewed in this paper. The mechanism of anticarcinogenic activity was discussed from the following 5 aspects: antioxidative activity, modulation on the key enzymes, blockage of signal transduction, antiangiogenic activity and the apotopsis of cancer cells. The developmental prospect and the investigational tendency of tea polyphenols in the future were discussed.
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    Cited: Baidu(43)
    Study on the Characteristic of Aroma Components in Green Tea with Chestnut-like Aroma
    YE Guo-zhu, JIANG Yong-wen, YIN Jun-feng, YUAN Hai-bo, ZHANG Rui-lian, WANG Zhi-lan, SHEN Dan-yu, WANG Fang, CHEN Jian-xin
    Journal of Tea Science    2009, 29 (5): 385-394.   DOI: 10.13305/j.cnki.jts.2009.5.009
    Abstract116)      PDF(pc) (570KB)(40)       Save
    E Mei MaoFeng tea with chestnut-like aroma was processed under different temperature and time to change the intensity and type of the aroma. The treated teas and E Mei MaoFeng tea were classified into two types—teas with chestnut-like aroma and others without chestnut-like aroma, according to the organoleptic evaluation. After analysis on the differences of the two types of teas using Duncan’s multiple tests, principal component analysis was applied to observe and analyze the distribution of the selected aroma components and the tea samples. The results demonstrated that the teas with chestnut-like aroma included remarkably high contents of β-ionone, nerolidol, phytol, 1,4-eicosadiene, 5,8,11,14-eicosatetraenoic acid, ethyl ester, (all-Z)-, 2,6-di-tert-butylbenzoquinone, pentadecane,2-methyl-, heptadecane, hexadecane,2,6,10-trimethyl-, but the content of dodecanol,3,7,11-trimethyl- was remarkably lower than the later. The ten aroma components have a good effect of distinguishing the teas with chestnut-like aroma from those without chestnut-like aroma. On the other hand, nonanal, and cis-jasmone are also worthy of attention.
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    Cited: Baidu(43)
    Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers
    Rajan Kumar Mishra, Swati Sen-Mandi
    Journal of Tea Science    2004, 24 (2): 86-92.   DOI: 10.13305/j.cnki.jts.2004.02.003
    Abstract67)      PDF(pc) (292KB)(22)       Save
    Tea plants growing in Darjeeling area, in addition to all other factors described below, are unique due to their typical geographical isolation. DNA fingerprints revealed a high degree of polymorphism in genomes of different clones, and this demonstrated the suitability of using the Amplified Fragment Length Polymorphism (AFLP) method for genome analysis between closely related plants, such as vegetatively propagated (clonal) populations of tea. Cluster analysis exhibited a dendrogram that closely matched with earlier clonal grouping based on morphological characters. The extent of genetic relatedness between the clones was found to be at 70% level. Results also showed that the genetic variation (Hs) was higher among China type. The variation between and within the three types of clonal populations studied (viz. China, Assam and Cambod type) are 63% and 36% respectively.
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    Cited: Baidu(42)
    Study on Chemistry and Bioactivities of Tea Polysaccharides
    XU Zhong-xi, WANG Kun-bo
    Journal of Tea Science    2004, 24 (2): 75-81.   DOI: 10.13305/j.cnki.jts.2004.02.001
    Abstract106)      PDF(pc) (280KB)(210)       Save
    The recent researches have found that tea polysaccharide has many bioactivities, such as anti-thrombosis, reducing blood-sugar, blood-lipid and pressure depressing and strengthening the body's immunity. It can be used in curing diabetes and cardiovascular disorder as healthy foods and subsidiary medicine. The methods of extraction and purification, determination of purity and molecular weight, chemical composition, and bioactivities of tea polysaccharide are summarized.
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    Cited: Baidu(39)
    Effect of Drying Methods on the Aromatic Character of Pu-erh Tea
    ZHANG Ling-zhi, CHEN Wei-xin, WANG Deng-liang, ZHOU Li-min, CHEN Yu-fen
    Journal of Tea Science    2007, 27 (1): 71-75.   DOI: 10.13305/j.cnki.jts.2007.01.011
    Abstract82)      PDF(pc) (374KB)(51)       Save
    With the aim to study the effect of drying methods on the aroma Characteristics of Pu-erh tea, the headspace solid-phase microextraction (HS-SPME) coupled with capillary gas chromatography-mass spectrometry (GC-MS) was used for the determination of volatiles in Pu-erh tea dried by baking, air-drying and sun-drying respectively. Results showed: Thirty-five compounds representing 95.4% of aroma contents of air-dried sample were identified with lower contents of terpenes and alcohols but higher contents of esters. Some biphenyls compounds which contributed to the stale odor of Pu-erh tea were distinct compounds of the air-dried sample. As for the sun-dried sample, Twenty-eight compounds representing 96.5% of aroma contents were identified with higher contents of ketones and lower contents of esters. In the baked sample, Twenty-four compounds representing 98.6% of aroma contents were identified with more alcohols compounds such as epoxylinalol, α-terpineol, 2-hydroxy-3,5,5-trimethyl-cyclohex- 2-enone contributed to the camerlsed and sweet flower odor of tea. The results were consistent with that of sensory tests. Sensory tests showed: The characteristic aroma of sun-dried tea, baked tea and air-dried tea were pungent, high-fired and stale odour, respectively. Results showed both baking methods and sun-drying methods were not suitable for Pu-erh tea, but the air-drying methods were the best method of the formation of Pu-erh tea aroma.
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    Cited: Baidu(39)
    Aroma Components of Oolong Tea by HS-SPME-GC-MS and GC-O
    MIAO Ai-qing, LU Hai-peng, SUN Shi-li, WANG Li, PANG Shi, LAI Zhao-xiang, ZENG Qiong, LIN Zhi
    Journal of Tea Science    2010, 30 (S1): 583-587.  
    Abstract79)      PDF(pc) (244KB)(58)       Save
    The aroma components of four Oolong teas were estimated by HS-SPME-GC-MS and GC-O. The results showed that the main volatile aroma components of Baiye Dancong were linalool and its oxide (I), indoles for Jinxuan, linalool, methyl salicylate, (Z)-hexanoic acid-3-hexenyl ester and alpha-farnesene for Tieguanyin, and linalool oxide (I), (Z)-hexanoic acid-3-hexenyl ester and alpha-farnesene for Huangjingui, respectively.
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    Cited: Baidu(36)
    Effect of Chemical Insecticides on the Diversity of Arthropods in Tea Garden
    WANG Guo-hua, LIANG Yuan-fa, TIAN Yong-hui, WANG Jia-lum, ZHOU Guo-lan
    Journal of Tea Science    2004, 24 (2): 99-104.   DOI: 10.13305/j.cnki.jts.2004.02.005
    Abstract67)      PDF(pc) (386KB)(28)       Save
    The effect of chemical insecticides on the structure and diversity of arthropods in various tea gardens were investigated. Results showed that the effect of chemical insecticides on the arthropods in tea gardens reflected in the structure and the constitution of species, the diversity index and the distributive evenness of species. The index of population density of dominant insect pest was increased greatly in those tea gardens with higher chemical insecticide expense, especially for tea leaf hopper (Empoasca vitis Gotho). The regulatory effect of natural enemies on major insect pest was increased in those tea gardens with lower expenses of insecticides. Besides, the diversity index and the evenness index were increased at the same time.
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    Cited: Baidu(36)
    Establishment of ISSR-PCR Reaction Conditions in Tea Plant
    YAO Ming-zhe, WANG Xin-chao, CHEN Liang, YANG Ya-jun
    Journal of Tea Science    2004, 24 (3): 172-176.   DOI: 10.13305/j.cnki.jts.2004.03.005
    Abstract83)      PDF(pc) (156KB)(50)       Save
    The optimal ISSR-PCR reaction conditions in tea was established by studying the main parameters. Results showed that the optimum concentration of five important components i.e. template DNA, primer, Mg++, dNTP, Taq DNA polymerase in 20βµl reaction mixture was 10ng, 150βnmol/L, 1.5βmmol/L, 150βµmol/L, 0.5βU, respectively. The appropriate annealing temperature was average 4.5℃ higher than Tm of corresponding primer, and at least 30 PCR cycles should be carried out to ensure sufficient PCR products. ISSR polymorphism between thirteen tea germplasm was 77.6%, and 12 out of 13 tea germplasm could be identified by ISSR fingerprinter established with primer TRI18.
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    Cited: Baidu(35)
    Extraction of Tea-polyphenols from Tea Leaves by Ultrahigh Pressure Technique
    ZHANG Ge, ZHANG Ling-ling, WU Hua, ZHANG Shou-qin
    Journal of Tea Science    2006, 26 (4): 291-294.   DOI: 10.13305/j.cnki.jts.2006.04.011
    Abstract119)      PDF(pc) (326KB)(143)       Save
    The extactrion of tea-polyphenols from tea leaves by using the ultrahigh pressure extraction technology (UPE) was studied. The experiment was designed with orthogonal method, and the yield of tea-polyphenols is used as the testing index. The extract conditions, including of the solvent concentration, pressure, the holding time of pressure and the soaking time before extraction, were investigated. The optimum extraction conditions are as follow, 60% ethanol, 200βMPa holding 3βmin., soaking 0.5 hr. The yield of tea-polyphenols is 28.92% under the optimum extraction condition. After separation, the yield of crude product of tea extraction is higher 25.3% than that of heat reflux method and the total of tea-polyphenols is higher 32% than that of heat reflux method. In addition, the extraction liquid of UPE is clear, easily to filter and no turbid after stored a long time. As a new extraction technology, UPE showed the great economic benefit if applied to extract tea-ployphenols from tea leaves in industrial scale.
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    Cited: Baidu(35)
    Comparative Analysis on Aromatic Components of Green Tea and Fresh Green Tea Beverage Using HS-SPME/GC-MS/GC-Olfactometry/RI Methods
    DOU Hong-liang, LI Chun-mei, GU Hai-feng, HAO Ju-fang
    Journal of Tea Science    2007, 27 (1): 51-60.   DOI: 10.13305/j.cnki.jts.2007.01.008
    Abstract87)      PDF(pc) (501KB)(56)       Save
    :Volatile components of green tea and fresh green tea berverage was extracted using HS-SPME and the major flavor compounds were identified by GC-MS/GC-Olfactometry/RI. The difference of aromatic constituents in composition and relative content between green tea and fresh green tea beverage was also compared.The results showed that the sort、intensity and contribution to total aroma of an aromatic compound could be identified and affirmed effectively by GC-MS/GC-Olfactometry/RI methods; The aromatic constituents of green tea were different from fresh green tea beverage in composition and relative content. Green tea contained 34 kinds of smell compounds, in which 3-Hexen-1-ol, 2-Ethyl-1-hexanol, Benzylalcohol, 1-Octanol, linalool, cis-Linalool oxide, Phenylethyl alcohol, Hexanal, (E)-2-Hexenal, Benzaldehyde, Acetophenone et al were the primary aromatic compounds. Fresh green tea beverage contained 37 kinds of smell compounds, in which linalool, trans-Linalool oxide, Phenylethyl alcohol, Geraniol, Nerol, Nerolidol, (Z)-2-Heptenal, Benzaldehyde, Acetophenone were the essential aromatic constituents.
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    Cited: Baidu(34)
    Genetic Diversity and Relationship of Huangjincha Cultivar Based on EST-SSR Markers
    YANG Yang, LIU Zhen, ZHAO Yang, LIANG Guo-qiang, ZHAO Xi
    Journal of Tea Science    2009, 29 (3): 236-242.   DOI: 10.13305/j.cnki.jts.2009.3.010
    Abstract72)      PDF(pc) (347KB)(56)       Save
    The 19 pairs of EST-SSR primers were used to analyze the genetic polymorphism and genetic relationship of 38 Huangjincha individuals. Totally 37 alleles were amplified using 19 EST-SSR primers, the number of alleles per primer ranged from 1 to 3, on average of 1.95. The expected heterozygosity (He) varied from 0.03 to 0.65, and the observed heterozygosity (Ho) ranged from 0 to 0.97, on average of 0.32 and 0.33, respectively. The Shannon index of the Huangjincha was 0.55. All of the results showed that the genetic polymorphism of Huangjincha was relatively poor. The 38 individuals were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.77. The results of the similarity coefficient among different individuals can give some implications to the genetic improvement of Huangjincha. At the same time, analysis of molecular variance (AMOVA) showed that variance component among groups and within populations were 21.77% and 78.23% each other, gene flow among the groups is 1.80, which all can give us some supports to protect the Huangjincha.
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    Cited: Baidu(33)
    Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction
    JI Peng-zhang, ZHANG Jun, WANG Ping-sheng, HUANG Xing-qi, XU Mei, TANG Yi-chun, LIANG Ming-zhi
    Journal of Tea Science    2007, 27 (4): 271-279.   DOI: 10.13305/j.cnki.jts.2007.04.001
    Abstract84)      PDF(pc) (544KB)(47)       Save
    Tea plant [Camellia sinensis var. assamica] populations in Yunnan province of China cultivated since ancient times are important genetic resources for future breeding of new tea cultivars. To evaluate the genetic diversity within and between Yunnan ten representative populations and to formulate conservation strategies for the ancient tea populations in Yunnan was conducted by ISSR technique in this study. The genetic diversity within populations calculated from Nei's genetic diversity (He) was estimated to be 0.281, on average, at the population level and 0.461 at the variety level, while Shannon indices (Ho) of population level and Assam variety level were 0.418 and 0.653, respectively and the percentage of polymorphic loci (P) of the ten populations ranged between 56.5% to 90.9%, respectively. Coefficients of gene differentiation (Gst) within the population was 0.391, in agreement with the result of Shannon’ diversity indices analysis (36.0%) and analysis of molecular variance (AMOVA) among population (39.7%), which illuminated that 60.9% of the variation in Assam variety were from the individuals of the population, and 39.1% of the genetic variation were from the population. The result indicated Camellia var. assamica variety possesses high genetic diversity and medium genetic differentiation was existed in the genetic variation between population. This could be caused by the highly outcrossing nature and habitat fragmentation of tea species. Conservation strategy are suggested including in situ strategies and ex situ strategies based on the observed genetic information of population.
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    Cited: Baidu(33)
    The Composition and Chemiluminescence Analysis of Theaflavins in Enzymatic Oxidation Products of Tea Polyphenols
    DING Zhao-tang, WANG Xiu-feng, YU Hai-ning, SHEN Sheng-rong
    Journal of Tea Science    2005, 25 (1): 49-55.   DOI: 10.13305/j.cnki.jts.2005.01.009
    Abstract57)      PDF(pc) (315KB)(20)       Save
    In this paper, theaflavins extracts from black tea and tea polyphenols were used as control, composition of theaflavins in enzymatic oxidation products was analyzed by HPLC, and the antioxidant activity was investigated by the technique of chemiluminescence. Results showed that, TF2A was the main composition in enzymatic oxidation products. In theaflavins extracts from black tea, there mainly existed four kinds of theaflavins and their contents were not greatly fluctuated. By employing CuSO4-Vc-H2O2-yeast and Fe2+-H2O2-yeast systems, antioxidant activity of enzymatic oxidation products was better than theaflavins extracts from black tea and tea polyphenols.
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    Cited: Baidu(32)
    Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities
    KOU Xiao-hong, JIANG He-yuan, CUI Hong-chun, ZHANG Jian-yong, GAO Qing-qing, YUAN Xin-yue, SHU Ai-min, LIU Xiao-hui, GAO Qi
    Journal of Tea Science    2008, 28 (3): 172-180.   DOI: 10.13305/j.cnki.jts.2008.03.011
    Abstract78)      PDF(pc) (341KB)(103)       Save
    Water-soluble green tea polysaccharides were separated and purified by Ultrafiltration membranes with pore diameter of 150 kD, 20 kD, 6 kD in turn. All the three fractions were further separated and purified by DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 column chromatography, and more than 20 green tea polysaccharide components totally were obtained. Their purity and molecular weight distribution were determined with high performance gel permeation chromatography (HPGPC) equipped with ELSD detector, and five components of homogeneous polysaccharide were obtained. The immunological activities of these green tea polysaccharides were determined by stimulated phagocytosis and nitric oxide production on RAW264.7 macrophage cell line of mice. Results showed that the tea Polysaccharides with 20 kD MW possessed significant immunological activities on NO production or phagocytosis of mice macrophage Raw 264.7 cells.
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    Cited: Baidu(31)
    A Comparative Study on Bioactivities of Tea Polyphenols and Chlorogenic Acid
    ZHANG Xing-hai, WANG Yue-fei
    Journal of Tea Science    2007, 27 (1): 39-44.   DOI: 10.13305/j.cnki.jts.2007.01.006
    Abstract72)      PDF(pc) (544KB)(45)       Save
    Objectiveto study the differences of bioactivities of tea polyphenols (TPs) and chlorogenic acid (CHA), methods of POV assay、spectrophotometric determination and MIC were used to compare the antioxidation、scaventing free radicals and antibacterial action of TPs & CHA. Resultsin the six weeks showed that TPs (100βµg/g、200βµg/g) could restrain the POV of lard below 10βmeq/kg, antioxidation rises with concentrations of TPs, as resemble the effect of CHA (81%). On the free radicals, CHA showed selective scaventing ability, IC50(DPPH、·OH) of CHA (81%) is 65βmg/L and 135βmg/L, and CHA (20%) is 327βmg/L and 1β432βmg/L, but TPs is 52βmg/L and 1908βmg/L; In the antibacterial action, TPs and CHA can obviously inhibit both staphylococcus aureus and escherichia coli., The MIC of CHA (81%) is twice as that of CHA (20%) on staphylococcus aureus, 500βμg/g and 1β000βμg/g respectively, that of TPs is 250βμg/g, on escherichia coli.. Conclusion:TPs (95%) exceeds CHA (81%) and CHA (20%) in the antioxidation、scaventing DPPH and antibacterial activity on staphylococcus aureus, but the reverse was in the scaventing ·OH. It was regarded that TPs may play a more important role than CHA in the food and drug.
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    Cited: Baidu(30)
    Study on the Expression of Caffeine Synthase Gene mRNA in Tea Plant
    LI Yuan-hua, JIANG Chang-jun, WAN Xiao-chun
    Journal of Tea Science    2004, 24 (1): 23-28.   DOI: 10.13305/j.cnki.jts.2004.01.004
    Abstract60)      PDF(pc) (434KB)(53)       Save
    The expression of caffeine synthase gene mRNA in tea plant by means of in situ hybridization was investigated. Results showed that the expressional sites is mainly located in the cell cytoplasm and nucleus of palisade tissue, but not in vacuole. The expressional sites of leaf vein are mainly in parenchyma and phloem, however, that in phloem is weaker. The expressional sites in young stalk are in phloem. The expressional sites in different cultivars are basically similar, but the expressional signal is different. Results also showed that the expressional signal of the first fresh leaf in spring is stronger than that in autumn. In terms of processing technique, the green teas which are spread 0.5 h、1 h、2 h showed apparent expressional signal, but the signal was not observed in the black teas withered for 6 h、8 h、10 h and Oolong teas shaken for 6 h、8 h、10 h.
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    Cited: Baidu(30)
    Investigation and Comparison of the Aroma Components in Guangdong Black Tea
    WANG Qiu-shuang, CHEN Dong, XU Yong-quan, YIN Jun-feng
    Journal of Tea Science    2012, 32 (1): 9-16.   DOI: 10.13305/j.cnki.jts.2012.01.002
    Abstract125)      PDF(pc) (911KB)(40)       Save
    For the purpose of studying the volatile aroma components in Guangdong black tea and determine the main aroma components, the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds in black tea samples and their contribution to the total aroma. Results showed that more than 50 aroma compounds were identified including alcohol, ester, aldehyde, ketone, terpene, hydrocarbon etc., among which the alcohol was the most important compounds and ester and aldehyde were next to flower-like, fruit-like, refreshing and sweet aroma were the main character of Guangdong black tea. Linalool, gerariol, methyl salicylate, linalool oxide and nonanal were the five most important compounds, representing 37.55%, 11.90%, 8.89%, 5.4% and 5.21% of the total aroma respectively. The alcohol was the most important compounds in Yingde tea population, the contents of aldehyde and ketone were more in Baimao tea population. Dancong and Fujian tea plant contained more terpene compounds.
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    Cited: Baidu(30)
    Analysis of Differential Expression Genes in Cold-induced Tea Plant
    ZOU Zhong-wei, FANG Wan-ping, ZHANG Ding, DUAN Yun-shang, LI Xing-hui
    Journal of Tea Science    2008, 28 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2008.04.011
    Abstract84)      PDF(pc) (515KB)(39)       Save
    The differential expression was investigated between cold-induced and normal environment in tea plant (Camellia sinensis) with cDNA-AFLP (cDNA Amplified Fragments Length Polymorphism). Expression profiles were generated around 86 special cDNA fragments (16 pairs primer, 256 primer combinations). Ten differentially expressed transcript-derived fragments were isolated. Cha1 revealed similarities (77%) to one kind of low temperature and salt responsive protein in Arabidopsis thaliana. Cha5 revealed similarities (89%) to Arabidopsis thaliana 60 S ribosomal protein L7 (RPL7B), ChaH8 had high similarities (79%) to Nicotiana tabacum A10 mRNA for stress-inducible H1 histone-like protein. Cha9 had high similarities to Populus EST from severe drought-stressed opposite wood. Other 6 fragments showed no homology to the known sequences in GenBank, perhaps they were new genes.
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    Cited: Baidu(29)
    The Development and Tendency on the New Technology Application in Tea Quality Evaluation
    ZHOU Yi-bin, WANG Jun
    Journal of Tea Science    2004, 24 (2): 82-85.   DOI: 10.13305/j.cnki.jts.2004.02.002
    Abstract99)      PDF(pc) (362KB)(72)       Save
    Basically, the quality of tea is evaluated by the tea experts through senses. The sensitivity of human’s sense, however, is liable to be changed by external factors, therefore, it is not easy to make an accurate evaluation through human’s sense. The paper presents the new research development in evaluating tea quality such as the shape of tea, the color of tea infusion, the aroma and taste of tea by instruments, especially the application of the machine vision to the shape of tea and the color of tea infusion, the electronic nose to the tea aroma and the electronic tongue to the taste of tea, it put forwards the tendency of the new technology application to tea quality evaluation.
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    Cited: Baidu(28)
    Studies on the Quality Chemical Components in Sichuan Brick Tea
    QI Gui-nian, TIAN Hong, LIU Ai-ling, SHI Zhao-peng
    Journal of Tea Science    2004, 24 (4): 266-269.   DOI: 10.13305/j.cnki.jts.2004.04.008
    Abstract81)      PDF(pc) (33KB)(34)       Save
    The contents and constituents of amino acids, catechins, caffeine, tea polyphenols and water extracts in raw material, fermented leaves, Kang Zhuan tea from Sichuan province were studied using amino acid auto-analysis and HPLC analysis. The content of amino acids, essential amino acids, theanine,catechins, caffeine, tea polyphenols and water extracts in raw materials were 1424.00βmg/100g, 547.00βmg/100g, 87.15βmg/100g, 27.63βmg/g, 1.30%, 8.18% and 26.94%,respectively; those in fermented leaves were 1590.00βmg/100g, 668.00βmg/100g, 67.62βmg/100g. 27.52βmg/g, 1.24%, 7.90% and 24.53% respectively, and those in Kang Zhuan tea were 1420.00βmg/100g, 529.00βmg/100g, 66.88βmg/100g, 13.65βmg/g, 1.27%, 5.99% and 23.92%, respectively.
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    Cited: Baidu(28)
    Chemical Communication Between Tea Plant-Herbivore-Natural Enemies
    CHEN Zong-mao, XU Ning, HAN Bao-yu, ZHAO Dong-xiang
    Journal of Tea Science    2003, 23 (s1): 38-45.   DOI: 10.13305/j.cnki.jts.2003.s1.008
    Abstract88)      PDF(pc) (435KB)(35)       Save
    The relation of chemical communication between the host-herbivore-natural enemies is the basis of existence of the member in the food chain. The chemical communication between tea plant and the three major insect pests (tea geometrid, tea aphid, tea leafhopper) as well as the seven natural enemies was investigated by the authors. Results indicated that the location of insect pest on the tea plant depended on the volatiles emitted from the tea shoots, such as the z-3-hexen-1-ol, linanool, n-pentanol etc. Bioassay and EAG estimation showed that these compounds showed strong attractiveness and electrophysiological response to tea pests, however, they showed only a weak activities to the natural enemies. The metabolism in tea plants was changed and released the specific synomone after damaged by the pests. For example, the damage of tea geometrid induced more C5-C6 aldehyde compounds liberated and tea aphid damage induced the liberation of benzyaldehyde. The damage of tea shoots by tea leafhopper induced the release of 2,6-dimethyl-3,7-octadien-2,6-diol and indole. These compounds showed a strong attractiveness and electro-physiological response to their respective natural enemies under a very low concentration of 10-6-10-9βg/ml, however, they showed only a weak or no activities to leafhopper. The enzymes in the oral regurgitate secreted by the tea pests was the elicitor inducing the release of specific volatile synomone from tea shoots. These synomones possessed the systemic activity of translocation in tea plant.
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    Cited: Baidu(28)
    Molecular Cloning and Sequence Analysis on cDNA of Cystatin Gene from Tea Leaves
    WANG Zhao-xia, LI Ye-yun, JIANG Chang-jun, YU You-ben
    Journal of Tea Science    2005, 25 (3): 177-182.   DOI: 10.13305/j.cnki.jts.2005.03.004
    Abstract101)      PDF(pc) (232KB)(31)       Save
    Two degenerate primers were designed according to the conserved region among the known plant cystatins. A cDNA fragment of 204βbp was amplified by RT-PCR (reverse transcription polymerase chain reaction) of total RNA extracted from fresh leaves of Tea plant (Camellia sinensis cv Longjing43). A full-length cDNA of the cystatin gene was obtained by 3’/5’RACE (rapid amplification of cDNA ends). The cDNA sequence of this 627βbp clone contained an open reading frame encoding a polypeptide of 101 amino acid residues with a predicable molecular mass of 11.026βKDa. The deduced amino acid sequence contained the motif QXVXG conserved among most members of the cystatin superfamily. By using the program of Blast on GenBank database, the sequence presented a high match with the cystatin genes from other plants, such as European chestnut, Cassava, Cowpea, Tomato, Soybean et al. All researched out sequences were all cystatins, so we can conclude that the cloned sequence is a member of cystatin gene from Tea plant.
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    Cited: Baidu(28)
    Studies on Lipid-depressing Function of Water Insoluble Tea Protein
    HUO Po, HUANG Guang-rong, ZHANG Xiao- hui, XIA Yong, JIANG Jia-xin, FU Jian-yun, ZHANG Ke-chang
    Journal of Tea Science    2005, 25 (2): 95-99.   DOI: 10.13305/j.cnki.jts.2005.02.003
    Abstract81)      PDF(pc) (211KB)(70)       Save
    Alkali extraction technology of water insoluble protein from tea was studied. The extracting rate of tea protein was around 61.1%. The crude tea protein was prepared by adjusting pH of the extract and its purity was 54.45%.The effects of water insoluble tea protein on decreasing blood lipids were studied on the hyperlipidemia rats. Results showed that the protein had significant effects on decreasing TC、TG、LDL-C、AI and R-CHR in the hyperlipidemia rats(p<0.05). It could also modulate the HDL-C indexes. The results confirmed that the water insoluble tea protein had remarkable effects on decreasing the blood lipid of hyperlipidemia and possibly is preventing atherosclerosis and coronary heart disease.
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    Cited: Baidu(27)
    Effects of Acidification and Organic Matter Accumulation on Lead Bio-availability in Tea Garden Soils
    ZHANG Ming-kui, FANG Li-ping, ZHANG Lu-qing
    Journal of Tea Science    2005, 25 (3): 159-164.   DOI: 10.13305/j.cnki.jts.2005.03.001
    Abstract117)      PDF(pc) (73KB)(24)       Save
    The increased accumulation of lead (Pb) in tea is widely concerned. The study on the factors influencing Pb concentration in tea is necessary for the production of high quality tea. In this study, soils of two tea gardens (Yellowish red soil and Red soil) were collected and incubated by adding acid and organic manure for studying the effects of acidification and organic matter accumulation on Pb bio-availability of tea garden soils. Water-soluble Pb, NH4OAc extractable Pb and chemical forms of Pb in the incubated soils were characterized. The results showed that soil acidification promoted transformation of other forms of Pb into exchangeable Pb, and thus increased water solubility and bio-availability of Pb in the soils. Accumulation of organic matter could activate Pb in soils with pH>4, whereas accumulation of organic matter decreased Pb bio-availability in lower pH soils (pH<4). The effects of pH and organic matter on Pb bio-availability were more significant in sandy soil than that in clayey soil.
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    Cited: Baidu(26)
    Study on the Key Technology and Equipment for Low Caffeine Tea Processing
    SUN Cheng, QUAN Qi-ai, JIN Shou-zhen
    Journal of Tea Science    2003, 23 (s1): 68-72.   DOI: 10.13305/j.cnki.jts.2003.s1.013
    Abstract175)      PDF(pc) (152KB)(48)       Save
    The technology of de-enzyming of tea leaf by hot water for the purpose of minimizing the caffeine in tea was discussed. Be combined with dehydration, rolling and drying, the tea had the style of the traditional roasting green tea and the caffeine contents were reduced as high as 67%. The major constituents could be as high as 82%. This leaf dredging up machine is characterized by the advantage of simple structure, convenient handing, high rate of taking off caffeine, and saving water and energy.
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    Cited: Baidu(26)
    The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme
    FU Dong-he, LIU Zhong-hua, HUANG Jian-an, CHEN Hui-heng
    Journal of Tea Science    2008, 28 (1): 62-66.   DOI: 10.13305/j.cnki.jts.2008.01.003
    Abstract86)      PDF(pc) (340KB)(45)       Save
    Fuzhuan Tea was extracted by chloroform, ethyl acetate, 1-butanol in turn. The effect of Fuzhuan Tea on the activities of metabolize enzyme was investigated. The results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity. The sample extracted by chloroform could restrain pancreatic lipase a little and the relative activity was 0.82. The water extracts, the sample extracted by ethyl acetate and 1-butanol showed obvious activation, that of the sample extracted by 1-butanol was the most active. The multiple was 2.54. All fractions of Fuzhuan Tea were active to α-amylase, among them, the sample extracted by ethyl acetate was the most active. The relative activiy was 4.09. The water extracts was the second one and its relative activity was 2.99. That of the layer of water sample was not obvious. The sample extracted by chloroform could restrain α-amylase a little and the relative activity was 0.88. The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to α-amylase and pancreatic lipase respectively.
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    Cited: Baidu(26)
    Study on the Status of Lead Content in Tea Gardens of Zhejiang Province
    SHI Yuan-zhi, MA Li-feng, HAN Wen-yan, RUAN Jian-yun
    Journal of Tea Science    2003, 23 (2): 163-166.   DOI: 10.13305/j.cnki.jts.2003.02.015
    Abstract63)      PDF(pc) (291KB)(20)       Save
    The Lead content in tea leaves from main tea producing areas of Zhejiang Province and its affecting factors have been investigated. The results showed that the air deposit was one of the main sources for lead in tea leaves coming from. It could decrease the lead content to a certain extent when fresh leaves were washed. The lead contents in tea leaves had a significantly positive relationship with the available lead contents in the soil, therefore, reducing the available lead content in the soil would be one of the effective ways to reduce the lead content in tea leaves.
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    Cited: Baidu(26)
    Effect of Processing on the Pb and Cu Pollution of Tea
    HAN Wen-yan, LIANG Yue-rong, YANG Ya-jun,SHI Yuan-zhi, MA Li-feng, RUAN Jian-yun
    Journal of Tea Science    2006, 26 (2): 96-101.   DOI: 10.13305/j.cnki.jts.2006.02.004
    Abstract90)      PDF(pc) (409KB)(67)       Save
    The effect of processing on the Pb and Cu pollution of tea was studied in this paper. Results showed that tea processing was one of the main pollution sources of Pb and Cu. The extent of pollution was varied with the working procedure, machine and its metal composing. Almost every working procedure, including fresh leaves spreading out or withering, deenzyming, rolling or cutting, fermentation and drying could increase the Pb and Cu concentrations of tea. However, rolling and spreading out were the most important procedures resulting in Cu and Pb pollution, respectively. With the increase of rolling pressure and time, the Cu and Pb concentrations steadily increased. Due to different procedures, especially the rolling pressure and time, the Cu and Pb concentrations of made teas were different, oven dried and roast green tea were the highest, followed by CTC (Crushing, Tearing, Curling) and Orthodox black tea, almost no change in Longjing tea processing. The pollution sources were quite different between Cu and Pb. Cu pollution was mainly from rolling machine and the Cu content of the machine. Cu pollution from rolling procedure accounted for 90% of total one. Pb pollution was mainly due to dust, which came from unclean ground and air in the workshop. Therefore, keeping clean in workshop and regulating the metal constitution of rolling machine could reduce the Pb and Cu pollution during tea processing. In addition, clearing the mature leaves and tea dust away after tea primary processing also could decrease the Pb concentration of tea.
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    Cited: Baidu(26)
    Study on Separation and Concentration of Tea Polysaccharides by Membrane System
    YIN Jun-feng, YUAN Hai-bo, GU Ji-ping, LIN Zhi-zhong, CHEN Jian-xin, TAN Jun-feng, WANG Fang
    Journal of Tea Science    2006, 26 (2): 108-111.   DOI: 10.13305/j.cnki.jts.2006.02.006
    Abstract103)      PDF(pc) (365KB)(58)       Save
    The 50βK membrane has been ascertained as the best membrane for separation and concentration of tea polysaccharides, and on the basis of the result, the treatment had been optimized, and the result showed that contents of total sugar reached 30.37%, and those of tea polysaccharides arrived at 16.89%. In compared with the control, the scavenging rate of ·OH were increased by 39.89%. The experiment showed that tea polysaccharides is separated and concentrated by membrane, and the activity of tea polysaccharides could be remained.
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    Cited: Baidu(26)
    Study on the Gallic Acid in Pu-erh Tea
    LU Hai-peng, LIN Zhi, GU Ji-ping, GUO Li, TAN Jun-feng
    Journal of Tea Science    2007, 27 (2): 104-110.   DOI: 10.13305/j.cnki.jts.2007.02.002
    Abstract108)      PDF(pc) (484KB)(142)       Save
    Gallic acid is a characteristic phenolic compound in Pu-erh tea with notable bioactivity. The content of gallic acid in Pu-erh tea, the change of gallic acid during the pile-fermentation, and the relation between extract fraction bioactivity and its gallic acid content were studied. Results showed that the average content of gallic acid in Pu-erh tea was 9.01βmg/g, but striking dissimilarity existed between the different samples; The gallic acid content first increased to some extent then decreased during the pile-fermentation of the solar green tea of [Camellia sinensis (Linn.) var. assamica (Masters) Kitamura]. It was found that the extracted fractions which rich in gallic acid showed higher antioxidative activities by DPPH radical scavenging assay.
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    Cited: Baidu(25)
    Influence of Different Enzyme Origin on the Enzyme-Catalysing Synthesizing of Theaflavins in Single and Bi-liquid Phases Systems
    GU Ji-ping, LIU Zhong-hua, HUANG Jian-an, SHI Zhao-peng, WANG Gui-xue
    Journal of Tea Science    2007, 27 (1): 76-82.   DOI: 10.13305/j.cnki.jts.2007.01.012
    Abstract82)      PDF(pc) (300KB)(43)       Save
    Synthesizing theaflavins (TFs) in single and bi-liquid phase systems was studied by various source of PPOs. The results showed that different effects could be gained in synthesizing the theaflavins by using the various source of PPOs. As to PPO from fresh tea leaves, the effect in bi-liquid phase system was more remarkable than that in single liquid phase system. The content of TFs in ethyl acetate layer in single liquid phase system, amounted at 33.74%, the synthesizing rate of TFs was 14.245%, while in bi-liquid phases system tests, the content of TFs in ethyl acetate layer was 39.74%, and the synthesizing rate of TFs was 31.792%. When the PPO was from pear, the effect in bi-liquid phase was slightly better than that in single liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 45.73%, the synthesizing rate of TFs was 18.799%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 40.20%, and the synthesizing rate of TFs was 22.11%. However, when the PPO from apple, opposite results was gained that the effect in single liquid phase was better than that in bi-liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 18.22%, the synthesizing rate of TFs was 13.34%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 11.56%, and the synthesizing rate of TFs was 6.935%.
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    Cited: Baidu(24)
    Study on the Toxicity Experiments of Fuzhuan Tea
    XIAO Wen-jun, FU Dong-he, REN Guo-pu, GONG Zhi-hua, XIAO Li-zheng, LIU Zhong-hua
    Journal of Tea Science    2007, 27 (4): 307-310.   DOI: 10.13305/j.cnki.jts.2007.04.008
    Abstract88)      PDF(pc) (326KB)(54)       Save
    The toxic experiment of Fuzhuan tea was carried out by using the extracted instant Fuzhuan tea as material in order to evaluate its safety for the mice and rat. The results showed that the LD50 of male mice and female mice was 14β700βmg/kg·bw and 19β600βmg/kg·bw respectively(the limit reliability was 10β600~36β000), and both of the Ames test and the semen teratogenicity results were negative. During the 30 days fed by the feeding mixed with 0.85%, 1.25%, 1.70% instant Fuzhuan Tea for the rat rats of each dose group grew well, the hemogram indexes of rats from each dosage, blood biochemical indexes and triglyceride, Urea anhydride, blood muscle anhydride and blood sugar indexes, ratio of spleen to body weight, kidney to body weight, and the histopathological characteristic of all test rats showed no significantly difference (P<0.05), which suggested Fuzhuan Tea was a no poisonous food.
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    Cited: Baidu(24)
    Study on Clarification of Oolong Tea Beverage by Ultra-filtration
    YIN Jun-feng, QUAN Qi-ai, LUO Long-xin, QIAN Xiao-jun
    Journal of Tea Science    2003, 23 (s1): 58-62.   DOI: 10.13305/j.cnki.jts.2003.s1.011
    Abstract105)      PDF(pc) (310KB)(58)       Save
    The Oolong tea juice was ultra-filtrated by using the plate-frame module and choosing three kinds of CA membrane with three pore diameter . Study on effect of different ultra-filtration process on the contents of main compounds including tea polyphenols, amino acids, caffeine, soluble sugar, pectin, protein etc in Oolong tea juices and colour difference, sensory quality, pH, ultra-filtration flux, etc. The results showed that ultra-filtration can clarify the Oolong tea juice effectively. The ultra-filtration can obstruct high-molecular mass that easy to participate higher retaining rate of the main compounds under the suitable ultra-filtration parameter. Sensory quality of the green tea juice ultra-filtrated was fine.
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    Cited: Baidu(24)
    Change of Polysaccharide During the Processing of Oolong Tea and its Structure Characteristic
    NI De-jiang, CHEN Yu-qiong, YU Zhi, ZHANG Yun, XIE Bi-jun, ZHOU Ji-rong
    Journal of Tea Science    2005, 25 (4): 282-288.   DOI: 10.13305/j.cnki.jts.2005.04.008
    Abstract70)      PDF(pc) (363KB)(32)       Save
    During the process of Oolong tea, the extraction rate of TPS and the contents of its neutral sugar and protein decreased, particularly notably after the third shaking, but the content change of uronic acid was less. It was also indicated that the inhibiting effects of polysaccharide on radicals increased before the third shaking, and then decreased on Zuoqing stage. According to the changes of polyphenol content, when it was retained 85%, the content of TPS was highest and the effect on scavenging •OH and was best, which was regarded the technical index to end shaking during the process of Oolong tea. Four fractions of OTPS was obtained by DEAE-52 column chromatography, and there were obvious differences not only in contents of neutral sugar, uronic acid and protein of OTPS, but also in inhibiting effects on hydroxyl radical (•OH) and superoxide anion radical ( ). OTPS 2-1, a main fraction of OTPS, was obtained by DEAE-52 and Sephadex column chromatography. It was found that OTPS2-1 was a trinal compound of polysaccharide containing rich uronic acid and less protein. The results also showed that the molecular average-weight of OTPS2-1 was 8.877?104, and it was composed of Gal, Glu, Ara, Fuc and Rha with a molar ratio of 7.58:2.14:7.05:1.76:1.02.
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    Cited: Baidu(24)
    Chemical Forms of Lead and Cadmium in Soils from Some Tea Gardens near Highway
    ZHANG Ming-kui,HUANG Chang-yong
    Journal of Tea Science    2004, 24 (2): 109-114.   DOI: 10.13305/j.cnki.jts.2004.02.007
    Abstract69)      PDF(pc) (358KB)(69)       Save
    Three soil profiles and twenty-three surface soil samples, developed from various parent materials, were collected from different tea gardens near highway in Zhejiang province. The total, five chemical fractions (exchangeable, carbonate bound, organic matter-bound, oxides bound and residual forms) and water-solubility of Pb and Cd in the soils were characterized. The results showed that concentrations of total and different chemical fractions of Pb and Cd in the soils varied with locations and soil properties. Increased Pb and Cd concentrations in surface layer of the soil profiles suggested that Pb and Cd accumulation in the soils was related to soil pollution and fertilizer application. The concentrations of organic matter-bound, oxides bound and residual forms of Pb and Cd were mainly controlled by total Pb and Cd in the soils. However, exchangeable and water-soluble Pb and Cd, which had high potential risk, increased with decreasing pH. The results indicate that long-term plantation of tea could cause sol acidification and elevated concentrations of bio-available heavy metals in the soil, and hence aggravated the risk of heavy metals.
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    Cited: Baidu(23)
    Characteristics of Soil Nutrients and Enzyme Activity of Ancient Tea Garden in Jingmai, Lancang, Yunnan Province
    JIANG Hong, SHA Li-qing
    Journal of Tea Science    2008, 28 (3): 214-220.   DOI: 10.13305/j.cnki.jts.2008.03.008
    Abstract66)      PDF(pc) (275KB)(33)       Save
    Ancient tea gardens in Yunnan Province are regarded as the provenance of Pu-erh Tea, and the ancient tea garden (ATG) in Jingmai, Lancang County is a typical example of cultivated ATGs. In order to investigate soil nutrients and enzyme activity, the ATG soil in Jingmai and its neighboring conventional tea garden (CTG) and forest soils were sampled. Soil pH, organic matter (OM), total N (TN), total P (TP), total K (TK), alkaline hydrolyzed N (AN), available P (AP), available K (AK), Ca, Al, Mg, activity of acid phosphatase, catalase, urease and saccharase were analyzed. The results showed that (1) All the soils were acid and at a pH of 3.80~3.91, and pH in tea garden soils was lower than in forest soil. (2), The OM, N, P and soil enzyme activity were rich in top soil (0~20 cm) and poor under 20 cm in different soil layers, while K, Mg and Al showed no obvious difference in different layers. (3) OM was rich in all soils, and the contents of OM, TN and AN were CTG < ATG < forest, while TP and AP were richest in ATG. (4) Enzyme activity showed different distribution patterns in the three soils. (5) Soil pH correlated with metal elements, N existed the most significant correlation with other nutrients, and soil nutrients did certain correlation with soil enzymes, especially N, C, P, K.
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    Cited: Baidu(23)
    Advances on DNA Molecular Markers and Gene-engineering in Tea Plants
    WANG Li-yuan, CHENG Hao, ZHOU Jian
    Journal of Tea Science    2004, 24 (1): 12-17.   DOI: 10.13305/j.cnki.jts.2004.01.002
    Abstract123)      PDF(pc) (333KB)(80)       Save
    Advances on gene-engineering in tea plants are reviewed from following aspects: DNA molecular markers, isolation and cloning of genes, genetic transform system. The problems and probable development in future are also prospected.
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    Cited: Baidu(23)
    Influence of Microwave-assisted Extraction on the Composition and Yield Rate of Catechins in Green Tea
    LI Min, REN Guo-pu, CUI Li, XIAO Li-zheng, XIAO Wen-jun
    Journal of Tea Science    2011, 31 (2): 112-118.   DOI: 10.13305/j.cnki.jts.2011.02.006
    Abstract85)      PDF(pc) (314KB)(38)       Save
    Microwave-assisted extraction of green tea showed certain effect on catechins yield rate and composition of catechins, however, it lack of systematic research. Based on systemic investigation on the influence of extracting power, extracting time, ratio of tea and water, water temperature and extracting times of microwave-assisted extraction on the composition and yield rate, the test processing parameters with the least influence of microwave extraction and higher yield rate on the composition were optimized and screened, which provide the reference for application in the industrialization of microwave-assisted extraction on tea by using the green tea as the raw material and the composition and yield rate in the extraction as the major index and used the industrial water as the control and the orthogonal test as the method of verification. Under the above mentioned parameters, the microwave-assisted extraction of green tea showed the least influence. The results showed that the optimum parameters were obtained at the ratio of solid to liquid of 1﹕25, microwave-assisted extracting power of 539W, microwave extracting time of 2min, extracting water temperature of 90℃, and the two times of extracting. The rate of EGC﹕DL-C﹕EC﹕EGCG﹕GCG﹕ECG was 3.25﹕0.12﹕1.00﹕7.66﹕0.49﹕1.82,the yield rate of the individual catechins was 130.47%、22.98%、124.62%、114.40%、7.39%、96.64% of yield rate extracted by industrial water. Compared with the current industrial technology, this method showed better practicability and results for the extraction of tea polyphenol and catechins.
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    Cited: Baidu(22)
    Adsorption Kinetics of Catechins onto Tea Stalk Lignocellulose
    YE Jian-hui, JIN Jin, LIANG Hui-ling, LIANG Yue-rong
    Journal of Tea Science    2008, 28 (5): 313-318.   DOI: 10.13305/j.cnki.jts.2008.05.004
    Abstract100)      PDF(pc) (320KB)(45)       Save
    Lignocelluloses prepared from woody tea stalk was used to adsorb tea catechins from green tea extract solution by static adsorption, focusing on the study of adsorption isotherm equation and characteristics of kinetics. The results showed that the Freundlich isotherm equation could be used to describe the adsorption of total catechins, and the adsorption capacities of total catechins decreased with the increase in temperature. The equations of pseudo-first-order model and pseudo-second-order model were used for the simulation of adsorption kinetic study. The kinetic results were more fitted to the pseudo-second-order model than the pseudo-first-order model. The saturated adsorption capacities on catechins calculated by the pseudo-second-order model were close to those of experimental data at different temperatures. With the increase in temperature, the rate constants of k1 and k2 are increased, while the initial adsorption rate of total catechins are decreased.
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    Cited: Baidu(22)
    Aluminium in Tea Soils, Rhizosphere Soil and The Characteristics of Al Uptake by Tea Plant
    RUAN Jian-yun, WANG Guo-qing, SHI Yuan-zhi, MA Li-feng
    Journal of Tea Science    2003, 23 (s1): 16-20.   DOI: 10.13305/j.cnki.jts.2003.s1.004
    Abstract77)      PDF(pc) (288KB)(53)       Save
    This paper summarized several experiments investigating aluminium in tea soil, rhizosphere and its uptake by tea plants. The main points are as follows. 1. The content of exchangeable Al (1βmol/L KCl) ranged from 2.5 to 988βmg/kg with an average of 238βmg/kg. Amorphous and interlay Al were the main fractions. Tea planting intended to increase of water soluble and exchangeable Al fractions. 2. The content of exchangeable Al increased with the amount of applied nitrogen as urea. Exchangeable Al in rhizosphere (1 mm from the root surface) increased significantly when NH4+-N was supplied. 3. Al absorption by intact tea root was a saturation process with a fast phase at the initial stage and lagged behind after some 6 h. 4. Al below 400βµmol/L promoted the growth of root, especially increased the number and total length of lateral roots.
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    Cited: Baidu(22)
    Properties of Rhizosphere Soil of Tea Plants and The Impact of Nitrogen Fertilizer
    RUAN Jian-yun, MA Li-feng, SHI Yuan-zhi
    Journal of Tea Science    2003, 23 (2): 167-170.   DOI: 10.13305/j.cnki.jts.2003.02.016
    Abstract67)      PDF(pc) (240KB)(26)       Save
    The properties of rhizosphere soil of pot-cultured tea plants were investigated. The rhizosphere soil was collected by removing the soil adhering to plant roots and other soil was referred as bulk soil. N fertilization with NH4+ (urea or (NH4)2SO4) significantly reduced soil pH, the contents of exchangeable Ca, K, Mg and base saturation whereas raised extractable Al levels. Compared to bulk soil, rhizosphere soil showed lower pH, exchangeable Ca, Mg and base saturation but a higher exchangeable K. The contents of soil solution Al, exchangeable Al and absorbed hydroxy Al were much higher in the rhizosphere soil than those in the bulk soil. The results showed that the acidification of rhizosphere soil increased the availability of Al, which might be of importance for tea plants capable of taking up a large amount of Al.
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    Cited: Baidu(22)
    Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant
    ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang
    Journal of Tea Science    2008, 28 (6): 459-467.   DOI: 10.13305/j.cnki.jts.2008.06.006
    Abstract62)      PDF(pc) (527KB)(22)       Save
    1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protein sequences of tea plant ACO aligned with those of other 12 plants showed highly conservative sequences in tea plant. Neighbor-Joining phylogenetic tree based on ACO sequence of tea plant and other 22 plants indicated that tea plant had very close relationship with that of Diospyros kaki. The ACO gene expression level of different cultivars after high and low temperature stresses were analyzed using RT-PCR method. The expression level has correlation with resistance of the cultivars to certain degree.
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    Cited: Baidu(21)
    Study on the Optimum Machine-plucking Period for High Quality Tea
    LUO Yao-ping, TANG Meng, CAI Wei-zhi, WEN Dong-hua, WEN Zheng-jun
    Journal of Tea Science    2008, 28 (1): 9-13.   DOI: 10.13305/j.cnki.jts.2008.01.007
    Abstract92)      PDF(pc) (260KB)(27)       Save
    On the basis of requirements for producing high quality tea, the seasonal variation of mechanical compositions of machine-plucked tea shoots under three plucking period was investigated when the following three criterions for tea shoots was pre-set up in one bud with one leaf and one bud with two leaves, and two-leaf banjhi. Results showed that high quality tea could be produced when the tea shoots were plucked in the period that 75.0%~84.9% shoots were met the one bud and tow leaves stage, and therefore this period can be defined as the optimum machine-plucking time. It was also showed that if machine-plucking is performed based on the above-defined criterion, the interval of machine-plucking in spring would be around 20 days, if an optimum period of machine-plucking is defined on the basis of the formation of a fish-leaf, it could be 15 days after the formation of fish-leaf in spring and 12 days in autumn. The average maturing time of a tea-leaf is 5.4 days in spring and 4.2 days in autumn in Hangzhou. Thus, a suitable regulation of machine-plucking period, will obtain the required plucked tea shoots for the manufacture of high quality tea.
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    Cited: Baidu(21)
    Effect of Exogenous Methyl Jasmonate-induced Tea Volatiles on Host-selection Behavior of Insects
    GUI Lian-you, CHEN Zong-mao, LIU Shu-sheng
    Journal of Tea Science    2004, 24 (3): 166-171.   DOI: 10.13305/j.cnki.jts.2004.03.004
    Abstract82)      PDF(pc) (46KB)(50)       Save
    The effects of volatiles from tea plants induced by exogenous methyl jasmonate (MJA) spraying or MJA-exposure on host-selection behavior of the parasitoid Apanteles sp. and the geometrid Ectropis obliqua as well as the percentage of E. obliqua caterpillars parasitized by Apanteles sp. under field condition were studied. Tea volatile organic compounds (VOC) induced by exogenous MJA-spraying or MJA-exposure exhibited a significant increase of attractiveness to female adults of Apanteles sp., but not to female adults of E. obliqua; and also significantly increased the percentage of E. obliqua caterpillars parasitized by Apantele sp in field condition.
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    Cited: Baidu(20)
    Study on the Composition and Stability of Soil Aggregates with Different Tea Plantation Age
    LIU Min-ying, ZHENG Zi-cheng, LI Ting-xuan
    Journal of Tea Science    2012, 32 (5): 402-410.   DOI: 10.13305/j.cnki.jts.2012.05.007
    Abstract86)      PDF(pc) (731KB)(8)       Save
    For the purpose of investigating the effect of tea plantation age on the soil aggregates composition and stability, the field data of tea planted area was collected and laboratory analysis was conducted. Results showed that: (1) In the dry and wet treatments dynamic changes were observed in the amount of soil aggregates with the change in clay diameter. It was found that the decreasing trend in the amounts of micro-aggregates was followed a decrease-increase-decrease trend with the decrease in clay diameter under dry treatment, and show a decrease-increase-decrease trend with the decrease in clay diameter under wet treatment. The amounts of micro-aggregates were decreased with the decrease of clay diameter. Results showed that there were no significant effects of tea plantation age on the soil micro-aggregates. (2) When the clay diameter was >5mm, it was found that the increasing trend in the amount of dry and water-stable aggregates was followed an increase decrease trend with the increase of planting time and the maximum value was found after 20~22 years of tea plantation age. At the same time <5mm of clay diameter displayed an opposite trend as compare to >5mm diameter. (3) The maximum value of MWD, GMD of dry-aggregates and water-stable aggregates were found at tea plantation age of 20~22, and the result is opposite for aggregate fractal dimension value (D value) of soil aggregates. The differences among GMD, MWD and aggregate fractal dimension were highly significant under different planting age, which indicated that the tea plantation age showed significant effect on the soil aggregate stability.
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    Cited: Baidu(20)
    A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV
    HOU Ru-yan, ZHU Xu-jun, ZHANG Zheng-zhu, CAI Hui-mei, WAN Xiao-chun
    Journal of Tea Science    2009, 29 (1): 67-71.   DOI: 10.13305/j.cnki.jts.2009.1.011
    Abstract83)      PDF(pc) (365KB)(45)       Save
    A method was developed for determination of imidacloprid residue in tea by high performance liquid chromatography (HPLC) with UV detector. Imidacloprid in tea samples was extracted with methyl alcohol and re-extracted by dichloromethane then cleaned up with the ENVI-18 cartridge. Imdacloprid was eluted with acetonitrile:water(v/v, 30:70) solution from the cartridge and detected with HPLC. The recovery of imidacolprid ranged from 81.5%~87.9% with the RSD less than 10.2%. Practical determination limit was 0.05 mg/kg.
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    Cited: Baidu(20)
    Study on the Content of EGCG3"Me in Different Tea Germplasms and its Changes
    LU Hai-peng, TAN Jun-feng, LIN Zhi
    Journal of Tea Science    2006, 26 (4): 310-314.   DOI: 10.13305/j.cnki.jts.2006.04.015
    Abstract77)      PDF(pc) (415KB)(41)       Save
    The changes of EGCG3"Me content in different tea germplasms, crop seasons and tea processing were studied by HPLC. The content of EGCG3"Me in six tested tea cultivars was more than 1% among 200 tea germplasms containing high tea polyphenols. The content of EGCG3"Me in new tea shoot was increased with the leaf maturity, and was higher in autumn tea than in summer tea. The content of EGCG3"Me was not changed obviously during the process of green tea manufacture, but increased distinctly during the withering process of the Oolong tea.
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    Cited: Baidu(20)
    Extracting Process of Teasaponin and the Application in Detergent
    LI Yun-tao, JIA Bin
    Journal of Tea Science    2006, 26 (3): 199-203.   DOI: 10.13305/j.cnki.jts.2006.03.009
    Abstract87)      PDF(pc) (248KB)(58)       Save
    A teasaponin is extracted from the tea seed, and then a well-behaved washing assistant is prepared by the teasaponin blended with sodium perborate. The influencing factor in the different extract method, and the application results were investigation. Results indicates, in the experiment terms, a satisfactory result were attained, under the experimental condition, and the detergent showed a good application results.
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    Cited: Baidu(19)
    Estimating the Quality of Tea Leaf Materials Based on Contents of Moisture, Total Nitrogen and Crude Fiber by NIR-PLS Techniques
    WANG Sheng-peng, WAN Xiao-chun, LIN Mao-xian, ZHANG Zheng-zhu
    Journal of Tea Science    2011, 31 (1): 66-71.   DOI: 10.13305/j.cnki.jts.2011.01.011
    Abstract90)      PDF(pc) (220KB)(47)       Save
    In order to estimate the quality of the fresh tea leaves by near infrared spectrum (NIRS) technique, the correlative model equation between near infrared spectrum of the fresh tea leaves and their corresponding moisture, crude fiber and total nitrogen amounts respectively through cross-validation and partial least squares (PLS) method were established in this paper. When the dimension was 7, the three indicators of R value were 0.92, 0.86 and 0.90, root mean square error of cross-validation (RMSECV) were 0.769, 0.332 and 0.742, respectively. A quality coefficient equation was suggested depending on the contents of moisture, crude fiber and entire nitrogen of fresh tea leaves. According to the quality coefficient equation of fresh tea leaf raw material, the quality coefficient of fresh tea leaves was obtained. The larger quality coefficient is, the higher quality of fresh leaf is. Thus, we developed a new method for the evaluation for the quality of fresh tea leaves by using NIRS was developed.
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    Cited: Baidu(19)
    Isolation and Identification of Thermophilic Fungi during the Fermentation of Puer Tea
    YANG Rui-juan, LU Jie, YAN Liang, YANG Liu-xia, LI Chen-chen, JIANG Shu, SHENG Jun
    Journal of Tea Science    2011, 31 (4): 371-378.   DOI: 10.13305/j.cnki.jts.2011.04.007
    Abstract80)      PDF(pc) (562KB)(54)       Save
    Thermophilic fungi were isolated and identificated by the method of sequencing during traditional fermentation of puer tea. Sampling were carried out every day. Many types of microorganisms including thermophilic fungi were purified and Identified. Isolated thermophilic fungi were cultured at different temperatures and different pH, the growth state of thermophilic microorganisms were observed. This article showed a comprehensive analysis of microorganisms during the fermentation of puer tea, and identified the isolated microorganisms using molecular biology method. Thermophilic microorganisms which play an important role during the fermentation were discovered, it laid the foundation to reveal the mechanism of Puer tea fermentation at high temperature.
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    Cited: Baidu(19)
    Selection of Optimum Conditions for Theaflavin Transformation from Tea Polyphenol through Immobilized Enzyme Membrane
    TU You-ying, FANG Qing, LIANG Hui-ling, HUANG Hai-tao
    Journal of Tea Science    2004, 24 (2): 129-134.   DOI: 10.13305/j.cnki.jts.2004.02.011
    Abstract72)      PDF(pc) (393KB)(29)       Save
    Immobilized polyphenol oxidases (IPPO) was applied for the synthesis of theaflavins using tea catechins as substrates. A Response Surface Methodological (RSM) study was conducted using a five-level, five-variable, central composite rotation design (CCRD) to select the optimum catalysis conditions for theaflavin transformation from tea polyphenol through immobilized enzyme membrane. Five important variables were considered, namely, incubation duration, enzyme/substrate ratio (E/S, Activity Units/ml-AU/ml), aeration volume, concentration of tea polyphenols, and the pH value of the reaction system. The highest theaflavins concentration of 0.766βmg/ml was predicted under the conditions as with 49βmin for the incubation duration, 128.7 for E/S ratio, 23.81βml/min ventilation for air volume, 5.95βmg/ml for catechins concentration, and pH 4.3. The measured theaflavins concentration was 0.754±0.017βmg/ml under the same condition, which suggested that the prediction model was practical and the selected conditions were reasonable.
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    Cited: Baidu(19)
    Taxonomic Study on the Dendrocola of the Tea Plantations in Southern Area of Henan Province
    LU Dong-sheng, WU Xiao-qin
    Journal of Tea Science    2004, 24 (4): 243-248.   DOI: 10.13305/j.cnki.jts.2004.04.004
    Abstract76)      PDF(pc) (39KB)(31)       Save
    The present paper is to report the classification of fungi on tea bushes in Southern part of Henan Province. Two hundred and thirty four specimens were collected by the author from 13 tea gardens, and 46 species in 27 genera belonging to 13 families have been identified. Among them, 17 species are new records of those which are living on tea bushes in China and 19 species are new records in Henan Province. The distribution and the bioecological habit of every species of the fungi are given.
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    Cited: Baidu(18)
    AFLP Analysis on Genetic Diversity of Fenghuang-Dancong Tea Plant Germplasms
    XIAO Li-zheng, YAN Chang-yu, LI Jia-xian, LUO Jun-wu, HE Yu-mei, ZHAO Chao-yi
    Journal of Tea Science    2007, 27 (4): 280-285.   DOI: 10.13305/j.cnki.jts.2007.04.002
    Abstract72)      PDF(pc) (714KB)(54)       Save
    Genetic diversity on 34 Fenghuang-Dancong tea plants were evaluated by AFLP fingerprinting using 5 primer combinations. The 5 primer combinations generated 438 bands and 87.6 bands were amplified by each primer pair on average, 348 bands(79.3%) polymorphic AFLP markers were detected, The result indicated that the genetic distance(GD) based on AFLP data by using DPS2000 was significant. The varience range of GD among the 34 Fenghuang-Dancong was 0.13~0.49. The GD(0.49) is highest between Zimaohuangzhixiang and Funanmilan and between Baiyedancong and Tongtianxiang, however, the GD(0.13) between Da-ansongcha and Zongsuojia is low. The dendrogram indicated that the genetic of 34 Fenghuang-Dancong germplasms have no certain relation with the aroma type.
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    Cited: Baidu(18)
    Study on the Activity of Dark Tea to Gastrointestinal Tumor
    SONG Lu-bin, HUANG Jian-an, LIU Zhong-hua, HUANG Hao, WANG Kun-bo
    Journal of Tea Science    2009, 29 (3): 191-195.   DOI: 10.13305/j.cnki.jts.2009.3.002
    Abstract86)      PDF(pc) (215KB)(39)       Save
    Stomach cancer and intestinal cancer are the two common diseases of the alimentary canal tumor. HCT-8 and SGC-7901 cell are taken as model to study the activity of Dark Tea to Gastrointestinal Tumor. The results showed that Dark Tea has good inhibitory action to gastrointestinal tumor cell growth. Dark tea contained two active inhibitors for Gastrointestinal Tumor, and the inhibitor for SGC-7901 cell has low polar than the inhibitor for HCT-8. The inhibiting active of Dark Tea to the two cell models maybe comes from manifold physiological activators, and maybe the low polar substances have stronger power.
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    Cited: Baidu(18)
    Effect of Cadmium Ions on Photosynthetic Structure and Its Functions of Tea Leaves
    SU Jin-wei, WANG Xiang-Ping
    Journal of Tea Science    2004, 24 (1): 65-69.   DOI: 10.13305/j.cnki.jts.2004.01.013
    Abstract93)      PDF(pc) (726KB)(37)       Save
    Tea plant was cultivated in Hoagland nutrient solution containing 0.5βmmol/L Cd2+ for 8 days. Tea leaves lost their green color gradually with the change in the ultrastructure of the chloroplast . At the early stage, there was a slight decrease in the number of granum thylakoids. Then, the thylakoids piled up loosely, and chloroplasts rounded off to different extents. At the late stage , the structure of the chloroplast related to thylakoid became unclear, while the thylakoid system was disintegrated abnormally with a few single bent and swell thylakoids, and some ruptured envelope of chloroplast. Cadmium ions significantly inhibited the photosynthesis activity and led to the decline in the chlorophyll content and chlorophyll a/b ratio. Further more the photochemical efficiency of PSII(Fv/Fm)and the potential activity of PSII(Fv/Fo)decreased in different degrees with increasing Cd2+ stress . The pollution duration resulted in the damage of photosynthetic structure in tea leaf cells.
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    Cited: Baidu(18)
    Study on the Changes of Non-galloylated Catechins and Relative Enzymes in Tea Shoots
    ZHANG Xian-lin, GAO Li-ping, XIA Tao, LIU Ya-jun, GAO Ke-jun
    Journal of Tea Science    2009, 29 (5): 365-371.   DOI: 10.13305/j.cnki.jts.2009.5.006
    Abstract69)      PDF(pc) (281KB)(28)       Save
    The changes of non-galloylated catechins and relative enzymes as well as the composition of catechins in different parts of tea shoots and in different seasons were investigated in this paper. The results showed that the contents of non-galloylated catechins except GC increased gradually from bud to the fourth leaf. Both of two synthetical enzymes related to non-galloylated cahechins, the activity of DFR/LAR declined gradually, but the activity of ANR was the highest in the third leaf. The result of correlation analysis showed that the contents of total catechins in different tea leaves presented a prominent positive correlation with the activity of DFR/LAR,but not with the activity of ANR.
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    Cited: Baidu(17)
    Cloning and Expression Analysis of Stress-resistant ERF Genes from Tea Plant [Camellia sinensis (L.) O. Kuntze]
    CHEN Lin-bo, FANG Chao, WANG Yu, LI Ye-yun, JIANG Chang-jun, LIANG Ming-zhi
    Journal of Tea Science    2011, 31 (1): 53-58.   DOI: 10.13305/j.cnki.jts.2011.01.009
    Abstract87)      PDF(pc) (269KB)(37)       Save
    For A TDF, which has been gained from genes expressed differentially in tea plant under cold stress using cDNA-AFLP, containing a complete coding sequence cDNA was cloned by RACE,named CsERF, and contains an ORF, which encodes a polypeptide of 212 amino acids including a conserved AP2 domains. Sequence alignment showed that CsERF protein shared high identity with other plants. qRT-PCR analysis showed that ERF gene was up-regulated by cold, ethylene, dehydration, NaCl and the maximum expression were 121.1, 22.6, 2.6, 2.2 times higher than before treatment, respectively. For different tea organs, transcriptional level of the ERF gene was the highest in mature leaves, the next was in bud, the third was in root and stem, and the lowest in flower and seed. It deduced that ERF gene was very important for tea plant in response to abiotic stress and was strictly controlled in expression of different organs.
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    Cited: Baidu(17)
    Effect of Al and F Interaction on the Tea Quality
    WANG Xiao-ping, LIU Peng, LUO Hong, XIE Zhong-lei, XU Gen-di, CHEN Li-jun
    Journal of Tea Science    2009, 29 (1): 9-14.   DOI: 10.13305/j.cnki.jts.2009.1.002
    Abstract69)      PDF(pc) (177KB)(24)       Save
    In this research, quality of tea leaves to aluminum (Al) and fluorine (F) interaction were studied, and five indices (polyphenols, caffeine, free amino acids, vitamin C and chlorophyll concentrations) in tea leaves (variety Anji Baicha and zhirenzaocha) were investigated after 30 d in hydroponic culture. The nutrient solution was treated by the interaction of Al (0, 30, 90 mg/L) and F (0, 4, 12 mg/L). The results were summarized as follows: With 30 mg/L of the single Al, the contents of polyphenols, caffine, free amino acids, vitamin C and chlorophyll in tea leaves were increased. But when the concentration of Al was 90 mg/L, the contents of above components in tea leaves were decreased. These components in tea leaves were increased under the treatment of 4 mg/L of the single F and decreased with the treatment of 12 mg/L of the single F. In the process of aluminum and fluorine interaction, the contents of these components in tea leaves of Anji Baicha were increased during the treatments of Al/F ratioin 30/4, 30/12, 90/4 and 90/12. However, zhirenzaocha were decreased. The results showed that tea quality improved in low concentration of aluminum or fluorine. Meanwhile, the variance effect of interaction was correlated to different proportion of Al/F and the proportion of Al/F for better quality of Anji Baicha and zhirenzaocha was different indicating there existed a varietal difference.
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    Cited: Baidu(17)
    Research Advance on Tea Biochemistry
    WAN Xiaochun, LI Daxiang, ZHANG Zhengzhu, XIA Tao, LING Tiejun, CHEN Qi
    Journal of Tea Science    2015, 35 (1): 1-10.   DOI: 10.13305/j.cnki.jts.2015.01.002
    Abstract118)      PDF(pc) (1093KB)(68)       Save
    Tea biochemistry is a subject of life science on Camellia Sinensis. It mainly focus on characteristic secondary metabolites of tea plants, especially those in the fresh tea shoots using biochemical and molecular techniques as research tools. Those metabolites include catechins, caffeine, theanine and volatile terpenes, which contribute greatly to tea quality and healthy effects. The major research fields of tea biochemistry involve the biosynthesis of secondary metabolites in tea plants, their transformation during tea processing and their effects on final tea quality and healthy function. Recently, there have several breakthroughs in the metabolites biosynthesis pathway, tea genome, specific tea germplasm metabolome, the processing metabolome, as well as the chemistry of tea quality. Since tea biochemistry is the fundamental in tea science, its achievements provide the theoretical basis and method approaches to tea cultivation and breeding, tea primary and further processing, tea trading and tea culture. With the development of tea industry and life science & technology, it is believed that the advancement of tea biochemistry will push forward tea industry to a high level at the sustainable development.
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    Cited: Baidu(17)
    Study on the Corpulent-reducing Function of Tea
    GONG Jin-yan, JIAO Mei, WU Xiao-qin, ZHANG Ying
    Journal of Tea Science    2007, 27 (3): 179-184.   DOI: 10.13305/j.cnki.jts.2007.03.014
    Abstract72)      PDF(pc) (398KB)(93)       Save
    Obesity may occur after long period of disorders in appetite and energy metabolism regulations. With the increasing of obesity incidence, searching for the high effective corpulent-reducing medicine became the common wish of research. Tea has been widely used as healthy drink for thousands of years, its function of corpulent-reducing has brought more and more attention all over the world. In this paper, researches of corpulent-reducing function of tea and its mechanism have been summarized.
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    Cited: Baidu(17)
    Study on Activity of Dark Tea Extracts on PPARs Model
    SONG Lu-bin, HUANG Jian-an, LIU Zhong-hua, HUANG Hao, WANG Kun-bo
    Journal of Tea Science    2008, 28 (5): 319-325.   DOI: 10.13305/j.cnki.jts.2008.05.002
    Abstract132)      PDF(pc) (329KB)(32)       Save
    Pu-er tea, Liu-bao tea, Fu-zhuan tea, Hua-zhuan tea, Qing-zhuan tea and Hei-zhuan tea as study material, and Tuo tea, Mi-zhuan tea as contrast material, the activity of dark tea on PPARδ and PPARγ model was studied. The results showed that the activation of dark tea to PPARδ & PPARγ receptor is high, and the activating components in dark tea on PPAR can be extracted by hot water. Therefore, dark tea showed a certain role on Hyperlipidemia, anti-obesity, regulation of carbohydrate metabolic and antiatherosclerosis.
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    The Anti-obesity Effects of Hubei Qingzhuan Tea on Rats
    CHEN Yu-qiong, ZHANG Wei, CHENG Qian, DONG Fa-jin, LIU Xiao-hua, GAN Duo-ping, QIAN Yu-ping
    Journal of Tea Science    2008, 28 (5): 363-369.   DOI: 10.13305/j.cnki.jts.2008.05.011
    Abstract86)      PDF(pc) (1319KB)(54)       Save
    The anti-obesity effects of Hubei Qingzhuan tea on rats were investigated. The results showed, compared with the obese control group, New Qingzhuan tea of this year(NQT) and the Qingzhuan tea which had been stored for 10 years(QTS) reduced the body weights of rats, Lee’s index and the ratio of body fat and mass significantly. Both teas possessed obvious effects on lowering the serum and liver level of TC, TG, MDA, increasing HDL-C levels and the activities of SOD, GSH-PX. The observation on the fat tissue showed that both teas could reduce the accumulation and volume of fat cells. As a results, New Qingzhuan tea had the effects of anti-obesity and antioxidation. The effects of anti-obesity increased with the dose adding of NQT, and the anti-obesity effects of QTS were better than that of the NQT.
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    Differences in Composition and Dynamic of Insect and Mite Community among Three Types of Tea Gardens
    HAN Bao-yu
    Journal of Tea Science    2005, 25 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2005.04.003
    Abstract93)      PDF(pc) (271KB)(49)       Save
    From July 2002 to July 2003, a survey on the species richness and abundance of insects and mites in the tea plant, the aerial space above the tea plant and on the ground from organic, non-pollution and common tea gardens were conducted in Magushan Mountains in the Southern Anhui Province. In total, 29018 individuals of 79 species from 41 families were recorded from a common tea garden, 35117 individuals of 81 species from 41 families were recorded from a non-pollution tea garden, and 12727 individuals of 102 species from 57 families were recorded from an organic tea garden. The tea green leafhopper, Empoasca vitis, abundance and its percentage accounted for the total abundance in organic, non-pollution and common tea garden were 5176 and 40%, 14049 and 40% as well as 17590 and 60%, respectively. Wasps, ground beetles, tiger beetles, rove beetles and ladybugs are the major natural enemies in tea gardens, whose species richness and abundance in organic, non-pollution and common tea garden were 40 and 2620, 33 and 1898 as well as 29 and 1610, respectively. In all the three types of tea gardens, species richness was higher from March to April and from September to October, and was lower from late November to middle January. Insect and mite abundance was high from April to June and from September to October, and was lower from July to August due to the warm weather as well as from December to January due to the cold weather. The abundance in the common tea garden fluctuated more than in other two types of tea gardens. Though the Insect and mite abundance was highest in the non-pollution tea garden, the pests did not cause serious damage because of the appropriate forecast and control. In the organic tea garden, the abundance was the lowest, while the species richness was the highest, and the energy paths were complicated and the community may be stable. At the present stage, most of the organic tea gardens in China are established among the mountains where vegetation is flourishing and biodiversity is high, such ecosystems may enhance natural control.
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    Study on the Extraction of Tea Juices from Fresh Green Tea Leaves
    ZHANG Ling-yun, LIANG Yue-rong, SUN Qi-fu, SUN Qing-lei, LU Jian-liang
    Journal of Tea Science    2003, 23 (1): 46-50.   DOI: 10.13305/j.cnki.jts.2003.01.010
    Abstract66)      PDF(pc) (297KB)(44)       Save
    An orthogonal experiment was applied to study the effect of extraction temperature, duration and ratio of tea to water on the quality of fresh green tea juice. The results showed that with rising of extracting temperature and extending of extracting duration, the extracted solids yield increased, the color of infusion turned to yellow and lightness of infusion decreased, and tea cream raised obviously. The optimum conditions of the extraction were as fallows: the ratio of tea to water 1:60, the temperature 50℃, and the extraction duration 10βmin.
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    Studies on the Optimal Water Condition for Tea Seedlings under Greenhouse Manufacturing Administration
    ZENG Jian-ming, GU Bao-jing, CHANG Jie, YUAN Hai-bo, WANG Li-yuan, DONG Fang-shuai, CHENG Hao, ZHOU Jian, GE Ying, Chen Shenglun
    Journal of Tea Science    2005, 25 (4): 270-274.   DOI: 10.13305/j.cnki.jts.2005.04.006
    Abstract130)      PDF(pc) (286KB)(36)       Save
    The photosynthesis, chlorophyll content and the growth traits of the clonal tea seedling grown under various substrates with different water content under the greenhouse were compared. The new biomass reaching their maximum values in the substrate with 80%~100% relative substrate water content, root mass ratio (RMR) decreased with the increase of water content of substrate, while the content of chlorophyll, specific leaf area (SLA), leaf area ratio (LAR), root emerging rate of the plant and specific root length (SRL) increased as the water content of substrate increased. The maximum photosynthetic rate (Pnmax) and new biomass increased as the water content of substrate increased, reaching their maximum values at 70%~100% water content of substrate. Content of chlorophyll increased with the rise of relative water content of substrate, while the ratio of chlorophyll a/b decreased with the substrate water content of substrate. Lower substrate water content can expedite the growth of tea seedling after rootage.
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    Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector
    LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an, GONG Yu-shun
    Journal of Tea Science    2005, 25 (3): 225-228.   DOI: 10.13305/j.cnki.jts.2005.03.012
    Abstract89)      PDF(pc) (316KB)(33)       Save
    High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector(ELSD-2000)was used for the determination of theanine in tea without derivatization. A Polaris-C18 column (250βmm×4.6βmm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution .The flow rate was at 0.5βml/min. The linear response ranged from 0.062~0.450βmg/mL for theanine. The recovery was 97.5%. The method was evaluated as accurate and simple.
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    Attracting Effect of Volatile Infochemicals from Tea Shoots and Flowers on Winged Tea Aphids
    HAN Bao-yu, ZHOU Cheng-song
    Journal of Tea Science    2004, 24 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2004.04.005
    Abstract65)      PDF(pc) (47KB)(19)       Save
    The attracting effects of the fifteen volatile components emitted from tea shoots and tea flowers on alate tea aphid, Toxoptera aurantii, were measured by four-arm olfactometer and electroantennogram (EAG). The result showed that in those odors (Z)-3-hexen-1-ol, (E)-2-hexenal, methyl salicylate, linalool, n-octanol and hexanal as well nerol and allyl isothiocyanate, the main aroma components from tea flowers showed significant attracting effect on the winged tea aphids. The difference in aphid number attracted by 2 doses of 10-4βg/ml and 10-2βg/ml of 15 components was obvious in olfactometer bioassay. EAG responses showed that: ① the EAG value by the odor from the fresh 1st leaf below the bud was largest; ② Simulating the “green leaf volatiles”, a mixture composed of 10-2βg/ml n-hexanol, n-pentanol, 1-penten-3-ol, 2-penten-1-ol, (E)-2-hexenal, (Z)-3-hexen-1-ol and (Z)-3-hexenyl acetate according to ratio of 1∶1∶1∶1∶1∶1∶1, could elicit strong EAG responses; ③ the odor from aphid-damaged tender stem repelled tea aphids. It was supposed that the green leaf volatiles and methyl salicylate liberated from tea shoots and tea flowers play a role of attracting lures in alate tea aphids foraging and orienting to tea shoots, and the aphid-damaged tea shoots maybe emit infochemicals to dispel tea aphids.
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    The Demonstration Research on Congregation Degree of Tea Industry
    SONG Yan-ping, WANG Yan-rong
    Journal of Tea Science    2008, 28 (5): 379-385.   DOI: 10.13305/j.cnki.jts.2008.05.012
    Abstract75)      PDF(pc) (314KB)(39)       Save
    Industry accumulation is an economic phenomenon of space congregation or local special congregation with the process of economic development. The paper calculates the congregation degree of AnHui tea industry in the period of 2002~2006 by using the means of LQ quotient and the index of concentration. The result indicated that Anhui tea industry possessed a signification congregation tendency and it’s mostly concentrated in the south and west regions of Anhui province. The result also indicated that the level of AnHui tea industry accumulation has a strong positive correlation to the industry development.
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    Research on the Correlative Factors of Sensory Quality of High-quality Green Tea
    WANG Tong-he, HU Min, ZHANG Jiu-qian, LI Hong
    Journal of Tea Science    2008, 28 (1): 33-38.   DOI: 10.13305/j.cnki.jts.2008.01.005
    Abstract80)      PDF(pc) (264KB)(54)       Save
    The factors of tea sensory evaluation by biological statistical software SAS9.0 were conducted according to the results of sensory evaluation from Tea Culture Festival in Xinyang city from 2004 to 2007. Results showed that the correlation between liquor color and aroma, taste, infusion was significant, but the correlation between appearance and the other factors was not significant. It was showed that the taste﹥aroma﹥liquor color﹥infusion﹥appearance in comparing with total quality score. The score proportion of color 23, aroma 23, taste 24, appearance 30 was rather reasonable. The correlative equation of each factor and total quality score was established by the method of stepwise regression with the DPS data processing software as follows: Y=25.8852+0.731X1+0.702X2+1.02X3+1.2204X4+1.03X5
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    Status and Suggestions of Tea Garden Fertilization on Main Green Tea-Producing Counties in Zhejiang Province
    MA Li-feng, CHEN Hong-jing, SHAN Ying-jie, JIANG Ming-bei, ZHANG Gen-miao, WU Lin-tu, RUAN Jian-yun, LV Jian-fei, SHI Yuan-zhi, PAN Li-xun, HUANG Cheng-mu, LIU Lin-ming, LIANG Bi-yuan, WANG Mei-qin, PAN Jian-qing
    Journal of Tea Science    2013, 33 (1): 74-84.   DOI: 10.13305/j.cnki.jts.2013.01.010
    Abstract80)      PDF(pc) (871KB)(63)       Save
    Fertilization status of typical green tea gardens in Zhuji, Shengzhou, Chun’an, Kaihua, Yongjia, Jiande, Changxing, Quzhou, Shaoxing, Songyang, Wuyi and Fuyang counties, and Xihu district of Zhejiang province in 2009 and 2010 was investigated. The result showed that fertilization of the investigated areas had several characteristics. Firstly, the main applied fertilizers were urea, compound fertilizer and organic manure (including rapeseed and livestock). Secondly, the fertilizers were mainly applied to soil surface in the way of broadcast. and compound fertilizer and organic manure were applied as topdressing. And period and frequency of tea gardens fertilizer application was random. Thirdly, the amount of N applied was deficient for 30% and excessive for 41% of tea gardens. Soil available P content was excessive for 50.4% of tea gardens. And soil available K content was deficient for 48.8% of tea gardens. Finally some fertilization suggestions are recommended according to tea garden application status.
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    ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province
    LIU Ben-ying, WANG Li-yuan, LI You-yong, TANG Yi-chun, HE Wei, CHENG Hao, WANG Ping-sheng
    Journal of Tea Science    2009, 29 (5): 355-364.   DOI: 10.13305/j.cnki.jts.2009.5.005
    Abstract81)      PDF(pc) (598KB)(34)       Save
    It was of much importance using minimum primers to obtain the maximum identification ability.Molecular identification of 134 tea germplasm resources using inter-simple sequence repeat (ISSR) markers was conducted in this study. There were 3 independent ways to identify tea germplasms: a) unique ISSR markers;b) unique band patterns and c) a combination of the band patterns provided by different primers.The result showed that the presence of 10 unique ISSR markers and the absence of 15 unique markers obtained from 12 primers made it possible to identify 21 tea germplasms, including ‘Xiangzhuqingdashancha’ and so on. Using 54 unique band patterns of primer UBC811 could identify 35 tea germplasms, including ‘Hainandayecha 1’ and so on. A1l the 134 tea germplasms could be entirely identified by the band patterns combination of primer UBC811, UBC835, ISSR2 and ISSR3, which was successfully used to construct the ISSR fingerprinting for discriminating 134 tea germplasms from Yunnan Province.
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    Effects of Different Extraction Processes on the Components of Tea Polysaccharides
    ZHOU Xiao-ling, WANG Dong-feng*, LI Su-zhen, ZHOU Xun, HOU Yang-feng, WANG Yuan-hong
    Journal of Tea Science    2007, 27 (1): 27-32.   DOI: 10.13305/j.cnki.jts.2007.01.004
    Abstract85)      PDF(pc) (498KB)(72)       Save
    Four different treatments including water-extracted process, pectase-extracted process, trypsin-extracted process and combined-enzyme-extracted process were respectively applied to extract tea polysaccharides (TPS) from Laoshan green tea in this article. The extract ratio of combined-enzyme process method reached 5.17±0.17 % and higher than those of the three other process methods. The crude TPS obtained from the four process methods were further purified through Sepharose FF and the main polysaccharides fraction was obtained respectively. Then comparisons on the components of monosaccharides, amino acids in the purified TPS were conducted. The four process methods had no influence on the kinds of monosaccharides in tea polysaccharides, but little on the proportion of the monosaccharisdes. The total sugar content of TPS prepared by the four process methods was decreased in following pectase-extracted process, combined-enzyme-extracted process, trypsin-extracted process, and water-extracted process. Especially, in pectase-extracted process it reached to 95.26±4.09%, while the uronic acids content decreased greatly. The four process methods had no influence on the kinds of amino acids in tea polysaccharides, but great on the proportion of them.
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    Anticancer Activity of Theaflavin Diagallate and its Mechanism
    JIANG He-yuan, Hang Xiao, YUAN Xin-yue, WANG Chuan-pi, Chung S Yang
    Journal of Tea Science    2007, 27 (1): 33-38.   DOI: 10.13305/j.cnki.jts.2007.01.005
    Abstract88)      PDF(pc) (406KB)(124)       Save
    Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC50 value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.
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    Effects of Vacuum Freezing Dry on the Aroma Quality of Oolong Tea
    YE Nai-xing, YANG Ru- xing, YANG Guang, YANG Jiang-fan, LIANG Xiao-xia, ZHENG De-yong
    Journal of Tea Science    2006, 26 (3): 181-185.   DOI: 10.13305/j.cnki.jts.2006.03.005
    Abstract79)      PDF(pc) (411KB)(44)       Save
    The vacuum freezing dry technique was applied during the drying process of the faint-scent type oolong tea. Compared with the freezing tea and baked tea, the contents of linalool, aromadendrene, β-caryophyllene, isovaleric acid phenethyl ester, β-cadinene, nerolidol, farnesol and hexatriacontane were higher in the freezing-dry tea. The vacuum freezing-dry technique could help to preserve the properties of color, aroma, flavor of oolong tea efficiently so that the freezing-dry tea had a strong flower scent, and its mellow was full of flavor. Therefore, the freezing-dry tea abowed a better quality in general compared with freezing tea and baked tea and would have a good prospective in the market in addition to its long storage and convenience in transportation.
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    Advances in Researches on Catechins Acylation Modification
    LIU Xiao-hui, JIANG He-yuan, ZHANG Jian-yong, YUAN Xin-yue, CUI Hong-chun
    Journal of Tea Science    2009, 29 (1): 1-8.   DOI: 10.13305/j.cnki.jts.2009.1.001
    Abstract79)      PDF(pc) (287KB)(55)       Save
    Researchers and the general public have been paid more and more attention to catechins due to their distinctive and extensive bioactivities, but the further applications are restricted because its liposoluble is not high, unstability and low bioavailability in vivo. This paper reviewed the research progress of acylation modificated catechins and their physiological activities as well as the prospects of development.
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    A Review on the Regulation Technique of Withering Process and the Change in Physical and Chemical Properties of Leaves
    HUA Jin-jie, YUAN Hai-bo, JIANG Yong-wen, LIU Ping, WANG Wei-wei
    Journal of Tea Science    2013, 33 (5): 465-472.   DOI: 10.13305/j.cnki.jts.2013.05.005
    Abstract100)      PDF(pc) (569KB)(156)       Save
    The withering is an important process of fermented tea, such as black tea and oolong tea, significantly affected the sensory quality of tea. With water constantly lost in the withering process, the respiration of fresh leaf is carrying on continuously, the endoplasmic components are also dramatically changed and the physical characteristics of withering leaves are also prompted to change. All of these provided specific substances and leaves basis for the follow-up processes and the quality of finished tea. This paper illustrates the dynamic variation of the physical properties and biochemical components in the withering process, and conducted a systemic review on the regulation and control technology on the key parameters of current withering. The facility withering technology, which can accurately control these parameters including environment conditions, such as temperature, humidity, light conditions and aeration conditions, will become the important direction of withering development in the future.
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    Evaluation of Nitrogen Status in Tea Plants by SPAD
    YANG Yi-yang, MA Li-feng, SHI Yuan-zhi, RUAN Jian-yun, LI Xing-hui
    Journal of Tea Science    2008, 28 (4): 301-308.   DOI: 10.13305/j.cnki.jts.2008.04.007
    Abstract92)      PDF(pc) (525KB)(120)       Save
    Determination of nitrogen concentrations in tissues is an important diagnostic means for N status of plants. A pot experiment with tea plants applied with different amounts of N fertilizer was carried out to evaluate the usefulness of SPAD-502 as a diagnostic tool. The results showed that the yield of young shoots and N concentration of mature leaves responded to N application rates with a pattern of an initial linear increase before a platform was reached. There was a significantly positive linear relationship between the SPAD reading and total N concentration of mature leaves. The similar pattern of an initial linear increase followed by a platform was observed for the relationship between SPAD reading and the yield. These results suggested that the N status of tea plants may be characterized by the measurement of SPAD reading of mature leaves and the SPAD is a potentially useful rapid diagnostic tool in N fertilization recommendation. However, this needs further validation from field experiments. Those factors which may influence the precision of SPAD measurement, such as the point of test on a leaf, selection of leaves from twig (position), weather condition, in situ or in vitro analysis, surface cleaning and the number of leaves for a composite sample were also investigated.
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    Fauna of Tea Fulgora and the Major Species in China
    ZHANG Han-gu
    Journal of Tea Science    2004, 24 (4): 240-242.   DOI: 10.13305/j.cnki.jts.2004.04.003
    Abstract89)      PDF(pc) (87KB)(101)       Save
    There were more than 20 species of fulgora recorded in tea areas of China. They belonged to 6 families of Fulgoroidea. Most of them belonged to Flalidae and Ricaniidae, including several harmful species.
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    Study on the Optimum Conditions in Extracting Polysaccharides in Sichuan Brick Tea
    GONG Fa-yong, QI Gui-nian, LI Jing
    Journal of Tea Science    2005, 25 (3): 229-236.   DOI: 10.13305/j.cnki.jts.2005.03.013
    Abstract84)      PDF(pc) (590KB)(39)       Save
    By means of orthogonally rotational combination design, a four-factor (temperature, time, ratio between tea and water, pH) substrate cultivation experiment was conducted by using of brick tea. The regression equations of the relationship between temperature, time, ratio between tea and water, pH and extraction rate of tea polysaccharides (TPS) were established. The results indicated that extraction rate of TPS was affected obviously in successive order by pH, temperature, ratio between tea and water and time. In range of experiment, extraction rate of TPS was increased obviously when pH and temperature were in higher levels, and the interaction of pH and temperature to the ratio between tea and water was significant, but the change of time and ratio between tea and water had less influence effects on extraction rate of TPS. Considering the high extraction rate and activity of TPS, the extraction conditions were optimized by frequency analysis that were 74.06~80.74℃temperature, 69.56~79.58βmin time, 38.49~42.59 ratio between tea and water, 7.411~8.284 pH.
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    Effect of Airflow on Zuoqing Environment and Quality of Oolong Tea
    JIN Xin-yi, WANG Xiu-ping, JI Ke-wen, MA Cui-lan, BAO Gui-lin
    Journal of Tea Science    2003, 23 (1): 41-45.   DOI: 10.13305/j.cnki.jts.2003.01.009
    Abstract75)      PDF(pc) (414KB)(42)       Save
    In order to prove up the effect of airflow on Zuoqing environment、physiological change of leaves and quality of Oolong tea, we determine on-line the air parameters including the air temperature, relative humidity, air quality and respiration rate of leaves under two air-conditioned Zuoqing environment respectively simulating direct pressure and all-around blast or airtight one during Zuoqing course. The experiment indicates that the air relative humidity and content of CO2 of leaf-layers and respiration rate of leaves under two airflow conditions showed ultimately marked difference. The quality of Oolong tea made under the blast environment is superior to that made under the other one.
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    Effects of Modifying pH on the Separation of Theaflavins by HSCCC
    JIANG He-yuan, CHENG Qi-kun, DU Qi-zhen
    Journal of Tea Science    2003, 23 (s1): 88-91.   DOI: 10.13305/j.cnki.jts.2003.s1.017
    Abstract98)      PDF(pc) (326KB)(38)       Save
    The four main kinds of theaflavins could be separated into three parts with the solvent system (Ethyl acetate / Methanol / Hexane / Water, 3/1/1/6) on HSCCC. The different pH zone of elutes could be appeared in the case of the difference of pH between sample solution and mobile phase. Due to the fact of the separation and purification of theaflavins are influenced by the pH of solution, and the process of separation and eluation is closely related to the pH of solution, so the method of modifying pH could be used to highly concentrate the four main kinds of theaflavins from the large scale of samples.
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    Study on the Variation of Cold Resistance of Tea Plant in Shandong Province
    SUN Zhong-xu, LIU Jing, QIU Zhi-lin, ZHAO Shu-ping, ZANG Ling
    Journal of Tea Science    2003, 23 (1): 61-65.   DOI: 10.13305/j.cnki.jts.2003.01.013
    Abstract70)      PDF(pc) (292KB)(36)       Save
    Clustering analysis of short distances on tea areas of ten cities and towns and six meteorologic factors showed that tea in Shandong is distributed mainly in four ecological regions. There are Rizhao and Qingdao, Linyi, Junan and Jiaonan of Qingdao; Taian and Xintai; as well as Laoshan of Qingdao and Zhucheng of Weifang. Analysis of variation indexes on tea in each ecological areas showed that frozen injury from light to heavy on tea dispersed piebald-style. Analysis of typical relativity showed that each meteorological factor is positively correlated with the change of tissue structure in tea leaf, among these meteorological factors, the most important factors on tea variation are annual rainfall and the extreme lowest temperature, followed by average annual temperature , frost-free period and sunshine hour, January average annual temperature in January showed little influence on it. With leaf structure the thickness of palisade tissue is most sensitive to climate, then the ratio of palisade tissue to spongy tissue and the thickness of spongy tissue. The thickness of leaf and the upper epidermis showed no relationship with the meteorologic factors.
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    Analysis of Thermal Efficiency during the Processing of Microwave De-enzyming of the Fresh Tea Leaves
    YE Yang, YUAN Hai-bo, YIN Jun-feng, LIN Zhi
    Journal of Tea Science    2004, 24 (4): 270-275.   DOI: 10.13305/j.cnki.jts.2004.04.009
    Abstract97)      PDF(pc) (43KB)(30)       Save
    It has not been reported the specific heat of fresh tea leaves that measured by the method of “mixing”. When the leaves contain 50% to 72% of the moisure the average heat ratio of the leaves is 3.44KJ/kg·℃. The results of the experiemnts showed that the main factor that affect the efficiency of heat is the quantity of the leaves during microwave de-enzyming. While the efficiency related with the thickness of the layer of tea leaves and methods for treatment need to be further study. The thermal efficiency of microwave de-enzyming is 32.32% to 38.62%, higher than that of machinery microwave processing, which is 21.23% and 18.11%. The experimental data may be useful to improvement of the efficiency of machinery microwave processing which is used in tea processing factory.
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    Studies on the Competitive Capacity and Tactics of Chinese Tea on the Overseas Market with SWOT Method
    JIANG Han-chun, WANG Xiao-gu, ZHAO Hong-ying, JIANG Su-han
    Journal of Tea Science    2004, 24 (3): 159-165.   DOI: 10.13305/j.cnki.jts.2004.03.003
    Abstract138)      PDF(pc) (46KB)(96)       Save
    Tea export plays a key role in Chinese tea economy. Tea has always the significance industrial value in both domestic and international markets. The export advantages and the weaknesses of Chinese tea, the opportunities and threats we are facing were analyzed by comparative principle on economy and SWOT method in this paper. The status and competitive capacity of Chinese tea in international tea markets was enunciated .The green tea and the special kinds of tea possessed competitive advantages must be developed in our country, the green marketing will be practiced, the criteria of domestic tea business will be in accordance with that of overseas ,moreover, the marketing of tea export must be unified.
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    HPLC Quantitation of Theaflavins in Tea Pigments
    LI Da-xiang, WAN Xiao-chun, LIU Li-hua, XIA Tao
    Journal of Tea Science    2004, 24 (2): 124-128.   DOI: 10.13305/j.cnki.jts.2004.02.010
    Abstract96)      PDF(pc) (274KB)(34)       Save
    In this study, theaflavin(TF1), theaflavin monogallate(TF2+TF3) and theaflavin digallate(TF4) in tea pigments are isolated and purified with high speed counter current chromatography combined with Sephadex LH-20 and semi-preparative HPLC. With the purified theaflavin, the standard curves have been set up by HPLC for theaflavins quantitation. The results on the accuracy and recovery determination showed that the coefficient of variation of TF1, TF2+TF3, TF4 and total theaflavins range from 2.6’8.5%, 2.0’3.8%, 2.0’3.9% and 2.2’4.1% respectively. Using HPLC quantitation, the relative error of total theaflavins amounts in the standard theaflavins from Sigma company is 3.4%. The total amounts of theaflavins in tea pigments are 20.5%, in which TF1, TF2+TF3 and TF4 are 6.0%, 9.1% and 5.4% respectively. This method is useful to quantify theaflavins and to control the quality of tea pigments, which could promote the studies on their pharmacological effects.
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    Analysis of Fatty Acid Composition in the Seed Oilfrom Fujian Tea Germplasm
    CHANG Yu-xi, ZHENG De-yong, YE Nai-xing, YANG Jiang-fan
    Journal of Tea Science    2012, 32 (1): 22-28.   DOI: 10.13305/j.cnki.jts.2012.01.011
    Abstract91)      PDF(pc) (732KB)(47)       Save
    The fatty acid compositions of 42 cultivars of tea seeds oil from Fujian province were determined by GC. The results indicated that the average content of saturated fatty acid (SFA) was 20.59%, the monounsaturated fatty acid (MUFA) was 50.57%, and the PUFA was 28.11%. The DHA with a mean of 0.11% was discovered in tea seeds. The investigated fatty acid compositions of fine cultivars were better than that in local cultivars. The major compositions of tea seeds oil were stearic acid, arachidic acid, palmitic acid and DHA. When 11 kinds of higher contents and special compositions were chosen as principal component analysis. The results showed that tea seeds oil possesses a high level of oleic acid, linoleic acid, and low levels of erucic acid and TFA. The tea seeds showed a potential value in the development of high grade edible oil.
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    Construction of Tea Caffeine Synthase Gene RNAi Vector
    ZHANG Guang-hui, LIANG Yue-rong, LU Jian-liang, DONG Jun-jie
    Journal of Tea Science    2006, 26 (4): 243-248.   DOI: 10.13305/j.cnki.jts.2006.04.002
    Abstract87)      PDF(pc) (222KB)(90)       Save
    Tea Caffeine synthase (TCS) is one of the key enzymes involved in caffieine biosynthsis in tea plant (Camellia sinensis),which catalyses conversions of 7-methylxanthine to theobromine and theobromine to caffeine. Inhibition of TCS gene expression can leads to breeding low caffeine tea cultivars. Two cDNA fragments of TCS gene were amplified by RT-PCR, and ligated into T-vector. The two TCS gene fragments were inserted into RNAi vector pFGC5941 in reverse direction after double digestion with two pairs of restriction endonucleases. The insertion of two fragments, namely pFGC5941-TCS02 and pFGC5941-TCS03, into the RNAi vector were confirmed by PCR and DNA sequencing.
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    Research Progress on the Health Functions of Tea Polyphenols and Development of Healthy Foods
    WU Xin-rong, WANG Yue-fei, ZHANG Shi-kang, XU Ping, YANG Xian-qiang
    Journal of Tea Science    2010, 30 (S1): 501-505.  
    Abstract74)      PDF(pc) (182KB)(35)       Save
    As a great natural antioxidant, tea polyphenols (TP) and its health function has been one of hot-topic studies at home and abroad. This paper reviewed the new outcomes of epidemiological and clinical studies on tea and health, which including prevention and treatment of cancers, cardiovascular diseases, neurodegenerative diseases and other diseases by TP. Meanwhile the current development and prospects on healthy foods of TP were also mentioned.
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    Progress on the Modification of Tea Polyphenols and Antioxidant Properties of Lipid-soluble Tea Polyphenols
    YING Le, ZHANG Shi-kang, WANG Yue-fei, ZHU Yue-jin, YANG Xian-qiang
    Journal of Tea Science    2010, 30 (S1): 511-515.  
    Abstract69)      PDF(pc) (180KB)(34)       Save
    Producing good quality lipid-soluble tea polyphenols (LTP) was the research focus at home and abroad. Tea polyphenols’ modification by solvent, emulsion and molecular modification were reviewed in this paper. The advantages and disadvantages among these three methods were compared. The antioxidant properties of liphophilic tea polyphenols and its mechanism were introduced. Its antioxidant properties in different antioxidant systems were compared. And the application of LTP as antioxidants was forecasted. At the same time, the developing direction of LTP production was proposed.
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    Determination of Total Fluoride in Brick Tea with Oxygen Bomb Combustion-Standard and Fluoride Ion Additive Selective Electrode Method
    WANG Xian-yang, LI Lu-ling
    Journal of Tea Science    2006, 26 (3): 204-208.   DOI: 10.13305/j.cnki.jts.2006.03.010
    Abstract81)      PDF(pc) (338KB)(56)       Save
    The method of determining total fluoride in brick-tea has been investigated, with oxygen bomb combustion-standard and fluoride ion additive selective electrode. The result showed that the sample of brick-tea was burned in the oxygen bomb with 3βMPa purity oxygen, then absorbing the ash with deionization water, the content of total fluoride was determined directly by means of fluoride ion additive selective electrode under pH5.6 and the concentration of sodium citrate dihydrate in TISAB is 110.0βg/L. The average recoveries were 95.88%~101.0% with RSD of 0.75%~1.25%. The fluoride in national tea reference material was determined, and the content of fluoride was accord with the certified values. This method is simple, rapid, accurate.
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    Study on Food Functionality of Tea Polysaccharides
    LI Lei, WANG Dong-feng, ZHOU Xiao-ling, DING Qing-bo
    Journal of Tea Science    2006, 26 (2): 102-107.   DOI: 10.13305/j.cnki.jts.2006.02.005
    Abstract95)      PDF(pc) (318KB)(69)       Save
    Two contents of tea polysaccharides, TPSⅠand TPSⅡ,whose content is separately 27.4% and 57.8%, were extracted from tea, and its food functionalities, such as solubility, capacities of oil absorption, moisture absorption and retention, frothing, froth maintenance and apparent viscosity, were investigated. Results showed that TPSⅠ and TPSⅡ could dissolve in water and not in organic solvents of high concentration, and they had capacities of oil absorption, frothing and froth maintenance as well as capacities of moisture absorption and retention in humid and dry environment. With the increasing of content of polysaccharides in TPS, apparent viscosity of TPS increased; and viscosity of TPSⅠ was four times as much as that of TPSⅡ.
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    Identification, Evaluation and Screening on Elite and Rare Tea Germplasms from Yunnan Province
    SONG Wei-xi, LIU Ben-ying, YI Bing, JIANG Hui-bing, MA Ling, DUAN Zhi-fen, SUN Xue-mei, WANG Yun-gang, WANG Ping-sheng
    Journal of Tea Science    2011, 31 (1): 45-52.   DOI: 10.13305/j.cnki.jts.2011.01.008
    Abstract85)      PDF(pc) (196KB)(52)       Save
    The agronomic traits, made tea quality, chemical components, cold tolerance and resistance to pests and diseases of 500 accessions tea germplasms collected from Yunnan Province were systematically and comprehensively investigated during 1986~2005. A total of 52 well-performed accessions tea germplasms were screened out according to nearly 100 characteristics. Among which, 32 accessions were relatively coordinated polyphenols, amino acids, caffeine and water extract contents, 2 super early maturity, 5 high polyphenols contents(>38%), 7 high caffeine content(>5.2%), 2 high caffeine content(<1%), 10 high theaflavins content(>1.6%), 26 high water extracts content(>48%), 2 high resistant to root knot nematode, 1 high resistant to tea leafhopper, 2 high resistant to tea red spider mite and 6 strong cold tolerance germplasms.
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    Tendency to Different Colors by Ricaniidae Insects in Tea Garden
    SUN Xiao-ling, CAI Xiao-ming, WANG Guo-chang, GAO Yu, WANG De-qiang, CHEN Zong-mao
    Journal of Tea Science    2011, 31 (2): 95-99.   DOI: 10.13305/j.cnki.jts.2011.02.003
    Abstract84)      PDF(pc) (338KB)(39)       Save
    The phototaxis is the basic behavior of numerous insects. Different species of insects has different preference of spectral range. Eighteen different colors were used to test the preference of Ricaniidae adults in the tea plantation. The results showed that yellow cards with high saturation were the first preference of Ricaniidae adults. Further investigation about field control efficacy of yellow sticky cards should be developed.
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    The Influence on Quality of Pseudosciaena Crocea Dip by Tea Polyphenol during Cold Storage
    ZHANG Xu-guang, LI Ting-ting, ZHU Jun-li, LI Jian-rong, LI Yu-jin
    Journal of Tea Science    2011, 31 (2): 105-111.   DOI: 10.13305/j.cnki.jts.2011.02.005
    Abstract75)      PDF(pc) (498KB)(72)       Save
    The effects of tea polyphenols (0.2%, w/V) mixture dip treatment on quality of Pseudosciaena crocea during cold storage were examined over a period index of 25d. Sensory characteristics, microbiological (total viable count), and chemical (pH, TVB-N, TBA, K-value) were assessed periodically for the treated group and the control. The results indicated that the shelf life were increased 7~8d at the storage of 4℃ under the tea polyphenols treatment. At the end of shelf life (15d), the total viable count was 106.12CFU/g and 107.45CFU/g, the pH value was 6.78 and 6.84, TBA was 0.12mg/kg and 0.16mg/kg, TVB-N was 166.6mg/kg and 318.7mg/kg, and K value was 27.10% and 43.72%, respectively. Therefore, tea polyphenols showed a significant inhibition effects on the propagation of bacteria and anti-oxidation of fat, thus prolonged the preservation time of Pseudosciaena crocea.
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    Pb Absorption and Accumulation in Tea Plants
    HAN Wen-yan, YANG Ya-jun, LIANG Yue-rong, SHI Yuan-zhi, MA Li-feng, RUAN Jian-yun, TANG Jia-yi
    Journal of Tea Science    2009, 29 (3): 200-206.   DOI: 10.13305/j.cnki.jts.2009.3.004
    Abstract79)      PDF(pc) (210KB)(41)       Save
    The characteristics of Pb absorption and accumulation in tea plants were studied by the analysis of Pb concentration in different parts of tea plant, in shoots of various seasons, cultivars and ages of tea plant. The results showed that feeding roots had the highest Pb concentration, followed by bud producing stems, lateral branches and old leaves, tender shoots and tea seeds were the lowest. Pb concentration in shoots and leaves increased with their maturity. Tea mature leaves and shoots had obvious seasonal change in Pb concentration with the highest during April to June and with the lowest in July for mature leaves, and spring > autumn > summer for shoots. A significant correlation was found between shoots and mature leaves in Pb concentration. A tendency was also found that the higher Pb concentration in shoots from the older tea plants. The Pb concentration in shoots from various tea cultivars was different to some degree.
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    Pesticide Residues in Tea and Their IntakeAssessment Using Brew Factor
    Anoop Kumar Barooah, Monorama Borthakur, Jatindra Nath Kalita, Tabaruk Hussain, Kasturi Chaudhury, Rajib Nath
    Journal of Tea Science    2011, 31 (5): 419-426.   DOI: 10.13305/j.cnki.jts.2011.05.009
    Abstract83)      PDF(pc) (507KB)(31)       Save
    Tea, the most popular beverage in the world, is produced from processed leaves of the plant Camellia sinensis. The tea plantations suffer from a large number of pests necessitating use of pesticides. In view of growing concerns for food safety, terminal residues of seven pesticides in processed black tea and their transfer into tea brew was studied. The residues of the pesticides in black tea ranged from 0.18 to 1.90mg/kg. Only a portion of these residues were transferred into tea brew, the form consumed. The actual intakes of residues through tea consumption, determined by incorporating a new parameter termed “brew factor” in the conventional estimates, were only up to 0.1% of the acceptable daily intakes for an average Indian. The compound specific brew factor improves risk assessment and will enable setting realistic maximum residue limits for pesticides in tea.
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    Phenotypic Diversity Analysis of Biochemical Composition in 52 Tea Germplasms
    WANG Xiao-ping, TANG Xiao-bo, WANG Ying-chun, LI Chun-hua, YAO Yu, LI Lan-ying, LUO Fan, WANG Yun
    Journal of Tea Science    2012, 32 (2): 129-134.   DOI: 10.13305/j.cnki.jts.2012.02.012
    Abstract61)      PDF(pc) (656KB)(38)       Save
    Biochemical components (including water extracts, polyphenols, amino acids, caffeine and catechins etc.) and genetic diversity in spring shoots were analyzed in 52 tea germplasms from Sichuan(33 germplasms) and other provinces(19 germplasms). The results showed that the biochemical diversity and variation were significant in these 52 tea gemplasms. Based on the biochemical composition data, 52gemplasms were clustered into two groups.
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    Determination of Theanine in Tea by Paired Ion Chromatography
    GONG Yu-shun, HUANG Jian-an, CUI Xiang-xing, LI Qing, LI Yin-hua, CHEN Jin-hua
    Journal of Tea Science    2008, 28 (2): 89-92.   DOI: 10.13305/j.cnki.jts.2008.02.004
    Abstract84)      PDF(pc) (248KB)(66)       Save
    A paired ion chromatographic method was developed for rapid determination of theanine in tea. SDS was added as a paired ion reagent, mobile phase was a mixture of acetonitrile and phosphoric acid aqueous solution, detection wavelength was set at 200 nm. The retention time of Theanine was 6.27 min. The calibration of theanine was in good linearity in the range of 0.04~20 μg with the regression equation Y=1.51466×106X+3.28706×105(r2=0.99901). The detection limits was 1.15 ng(S/N=3). This method is rapid, simple, and accurate for the analysis of theanine in tea.
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    Dynamics of Theanine Biosynthesis in Tea Suspension Cells
    CHENG Hao, GAO Xiu-qing
    Journal of Tea Science    2004, 24 (2): 115-118.   DOI: 10.13305/j.cnki.jts.2004.02.008
    Abstract72)      PDF(pc) (295KB)(40)       Save
    The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30th day and reached a high peak of 20% cell dry weight.
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    Derivation of Theanine and Its Determination by Capillary Electrophoresis
    LI Ping, WAN Xiao-chun, LI Jian, ZHANG Zheng-zhu, SHEN Zuo-jun
    Journal of Tea Science    2004, 24 (2): 119-123.   DOI: 10.13305/j.cnki.jts.2004.02.009
    Abstract86)      PDF(pc) (365KB)(78)       Save
    A novel derivative method for determination of theanine was developed by micellar electrokinetic capillary chromatography (MECC). Samples were derivated with 2,4-dinitrofluorobenzen. The buffer solution used in this method was 0.03mol/L sodium borate solution (pH9.8). Column temperature was 17℃. The separating voltage was 28βkV and detection at 360βnm. Factors of pH value, reaction time, reaction temperature and concentration of buffer were optimized. The products of derivation of theanine can be stable for five days at 4℃ or 25℃. The linearity of this assay was 0.2~5βmmol/L (r2=0.993). The minimal detection limit (S/N=2) was 0.05βmmol/L.
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    Studies on the Spatial Variability and Influencing Factors of Trace Elements in Sichuan Mengshan Montanic Tea Plantation Soils
    WANG Yong-dong, LIAO Gui-tang, LI Ting-xuan, ZHANG Xi-zhou, HUANG Ping
    Journal of Tea Science    2008, 28 (1): 14-21.   DOI: 10.13305/j.cnki.jts.2008.01.008
    Abstract67)      PDF(pc) (892KB)(57)       Save
    The subtropical hill region is the main area where the tea plantations are distributed in Chian, Trace elements are very important to tea quality, Study on the spatial variability of trace elements in tea plantation soils is the foundation of precision agriculture. With the original data as basis, the spatial variability of trace elements of montanic tea plantation soil in Mengshan is studied with the aid of principal component analysis and geostatistics model. The result showed: (1) The spatial variability of four trace elements(Fe, Mn, Zn, Cu) in tea plantation soils are similar. All of the theoretical model of trace elements are exponential model, the range of spatial correlation is 206~1 860 m. The spatial correlation of Fe, Mn, Zn belong to medium levels [C0/(C0+C): 25.1%~49.6%], the spatial variability between Mn and Cu similar anisotropic characteristic in NE111º, Fe and Zn trends to isotropy. (2) Principal component of comprehensive cartography describe the distribution rule of trace elements in general. Maps of Kriged estimates with principal component showed that the characters of trace elements distributed in a belt shape across the Mengshan mountain, which are in accordance with the result of geostatistic analysis. (3)The mainly influencing factors of tea plantation soil in Mengshan are soil condition and slope.
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    A Review on the Technique of Tea Decaffeination
    XU Yong-quan, YIN Jun-feng, YUAN Hai-bo, CHEN Jian-xin, WANG Fang
    Journal of Tea Science    2008, 28 (1): 1-8.   DOI: 10.13305/j.cnki.jts.2008.01.001
    Abstract105)      PDF(pc) (367KB)(44)       Save
    It is reported that tea has many medical functions. However, consumers prefer decaffeinated tea and tea products due to caffeine’s negative disadvantageous. Decaffeination is realized by different methods, including the traditional methods such as water decaffeination, solvent extraction, adsorption separating and supercritical carbon dioxide extraction. The recent developmental microbial and enzymatic degradation and breeding low caffeine tea varieties are also developed for removal of caffeine. The investigation on tea decaffeination was reviewed, and the feasibility was discussed in this paper. The techniques of water decaffeination combined with adsorption separating, microbial and enzymatic degradation as well as low caffeine tea breeding were regarded as the main developmental direction of tea decaffeination by the authors.
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    Rapid Propagation of Tea Clonal Seedlings in Auto-controlled Greenhouse
    CHENG Hao, ZENG Jian-ming, ZHOU Jian, WANG Li-yuan, CHANG Jie, GE Ying, YUAN Hai-bo, GU Bao-jing, ZHANG Xiao-fei
    Journal of Tea Science    2007, 27 (3): 231-235.   DOI: 10.13305/j.cnki.jts.2007.03.009
    Abstract74)      PDF(pc) (408KB)(54)       Save
    The rapid propagation of tea clonal seedling was carried out in auto-controlled greenhouse. The result showed that when the technical route of tissue culture-direct rooting-greenhouse incubating was followed, the optimal initial explant was the third axillary bud of spring shoots, which possessed the best germination rate, proliferated 2.75 times in one incubation cycle, and 20% of the cultures could grow to more than 5βcm length every cycles. On the other hand, when the technical route of direct greenhouse cutting and incubation was followed, biannual proliferation could be achieved with one started in middle March and harvested after 5 months while the height of seedlings reached 20.4±8.3βcm, the transplant survival rate of these seedlings was close to 100% after they were proper conditioned. The other proliferation started at about Aug. to Sept., after about 6 month’s incubation, the height of those seedlings reached more than 20βcm after the rootage was established during winter. The increment of seedling height of those fertilized was 5.9 times as much as those unfertilized.
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    Study on Blending of Made Tea with Mixture Design
    TONG Hua-rong, GONG Zheng-li
    Journal of Tea Science    2004, 24 (3): 207-211.   DOI: 10.13305/j.cnki.jts.2004.03.011
    Abstract74)      PDF(pc) (102KB)(34)       Save
    Blending program of 7 scented teas using Uniform mixture Design of experiments was studied. Results showed that Uniform mixture design of experiments could be used in blending of made tea effectively. With the model of responses, the interactions of sensory attributes among components could be estimated. With the aid of analysis software HL40, formulations of tea could be optimized for several responses and the best formulation could be gotten.
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    Studies on the Effect of Traditional Baking Process on Quality of Lingtou Dancong Oolong Tea
    WANG Deng-liang, GUO Qin, ZHANG Da-chun
    Journal of Tea Science    2004, 24 (3): 197-200.   DOI: 10.13305/j.cnki.jts.2004.03.009
    Abstract105)      PDF(pc) (35KB)(44)       Save
    The changes in the contents of the related bio-chemical components and the aroma constituents between final-firing sample and baking sample were analyzed. Results showed that the contents of the water soluble matters、polyphenols、amino acid、soluble sugar、TF and TR were decreased, and the contents of the TB were increased; concentration of aromatic constituents that showed aroma of flowers was decreased, and the concentration of aromatic constituents that showed aroma of honey was increased. That is to say that the baking process showed its key role in the formation of high aroma and mellow sensory tasting in Lingtou Dancong Oolong Tea.
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    Studies on the Green Fluorescence of Tea Polyphenols
    ZHANG Li-xia, JIA Ming, XIA Wen-juan, XIANG Qin-zeng, HUANG Xiao-qin
    Journal of Tea Science    2004, 24 (4): 288-294.   DOI: 10.13305/j.cnki.jts.2004.04.012
    Abstract65)      PDF(pc) (659KB)(23)       Save
    The effects of the concentration , pH, solvent, mental ion ( Ca2+ , Al3+ ) and heating on the fluorescence spectra of the tea polyphenols were studied. The results showed that the factors of all above had obviously effects on the fluorescent intensity of tea polyphenols. It reached the maximum intensity when pH of solvent is 7.4±0.2 or the concentration of tea polyphenols is 3βmg/ml or water solution contains 50% ethanol. Adding Ca2+ and Al3+ in the infusion of tea polyphenols reduced the fluorescent intensity, but it was mainly indirect effect by precipitating a portion of tea polyphenols. Heating treatment caused the fluorescent intensity of tea polyphenols higher. .The maximum emission peak would move to long wavelength when the concentration of tea polyphenols was above 3βmg/ml or pH>8, but other factors had less effects. Besides, solvent polarity affected the shape of excitation fluorescent spectra and the intensity obviously, but had less effect on the maximum emission wavelength.
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    The Main Active Component of Lipophilic Tea Polyphenols and in vitro Inhibition Activity on Ovarian Cancer Cells HO-8910
    CHEN Ping,ZHONG Jian-hua,SUN Dong
    Journal of Tea Science    2003, 23 (2): 115-118.   DOI: 10.13305/j.cnki.jts.2003.02.006
    Abstract77)      PDF(pc) (352KB)(40)       Save
    A single-substitution long-chain lipophilic catechin was isolated and purified from lipophilic tea polyphenols by high-speed countercurrent chromatography (HSCCC). Its molecular structure was elucidated as epigallocatechin-3-O-gallate-4'-palmitate by elemental analysis, IR, MS and 1H-NMR. The in vitro inhibition activity of the lipid-soluble epigallocatechin-3-O-gallate-4'-palmitate on ovarian cancer cells HO-8910 was investigated and compared with that of green tea polyphenols and lipophilic tea polyphenols. The results demonstrated that the inhibition activity of the single-substitution EGCG palmitate was almost corresponding to green tea polyphenols, and more effective than lipophilic tea polyphenols.
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    Review on Antioxidative Mechanism of Theaflavins
    CHEN Hu, HU Yin, ZHOU Rui, ZHAO Jian
    Journal of Tea Science    2005, 25 (4): 237-241.   DOI: 10.13305/j.cnki.jts.2005.04.001
    Abstract97)      PDF(pc) (247KB)(36)       Save
    The content of theaflavins is an important index of black tea’s quality, are the major substance in black tea, which show significant antioxidative effect. The antioxidative mechanism of theaflavins was revieved in this paper
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    Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant
    HONG Yong-cong, XIN Wei, LAI Yu-bin, WENG Xin, HU Fang-ping
    Journal of Tea Science    2005, 25 (3): 183-188.   DOI: 10.13305/j.cnki.jts.2005.03.005
    Abstract109)      PDF(pc) (352KB)(35)       Save
    The screening and identification of endophytic bacteria from the healthy and diseased leaves of various tea cultivar plants were conducted. Results showed that the endophytic bacteria were accounted in 2.9×106~39.4×106βcfu/(g•fw) in tea cultivars plants. The TL2 stain showed strongly antagonistic activity against leaf fungal pathogens of tea-plant. It showed a good protecting effect when inoculated the TL2 strain bacteria firstly than inoculated the tea grey blight pathogen(Pestallozzia theae) later, Besides, it showed a strong degrative activity to cypermethrin. The TL2 bacteria strain could colonize within tea plant. According to the results of identification, the TL2 bacteria strain was identified as bacillas subtilis.
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    Molecular Cloning and Sequence Analysis of Chalcone Isomerase Gene of Tea Plant (Camellia sinensis)
    MA Chun-lei, ZHAO Li-ping, ZHANG Ya-li, CHEN Liang
    Journal of Tea Science    2007, 27 (2): 127-132.   DOI: 10.13305/j.cnki.jts.2007.02.006
    Abstract63)      PDF(pc) (382KB)(47)       Save
    Tea is one of the most popular non-alcoholic healthy beverages in the world, which possesses great value as a source of secondary metabolic products, such as catechins. Isolation and cloning of important functional genes of tea plant (Camellia sinensis) is of crucial significance for using biotechnology method to regulate the metabolism of tea plant. In this paper, the chalcone isomerase gene, which was an important functional gene of catechins biosynthesis pathway, was cloned from tea plant by using EST sequencing and RACE (rapid amplification of cDNA ends) approaches. The full-length cDNA of chalcone isomerase gene is 1 163 bp (GenBank Accession No. DQ904329), containing a 723bp open reading frame (ORF) encoding a 240 amino acid protein, and its 3′ untranslated region has an obvious polyadenylation signal. The deduced protein molecular weight was 26.4 kD and its theoretical isoelectric point was 5.19. Sequence analysis result showed that it is closely related with that of Lycopersicon esculentum.
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    A Survey on the Trapping Effect of Four Types of Coloured Sticky Plates on Various Species of Insects in Tea Gardens
    XIANG Tai-hong, HAN Bao-yu, ZHOU Xiao-gui
    Journal of Tea Science    2007, 27 (3): 253-258.   DOI: 10.13305/j.cnki.jts.2007.03.005
    Abstract86)      PDF(pc) (394KB)(42)       Save
    In order to evaluate the entrapping effect of the bud green, the olive yellow-green, the jasmine yellow and the snow white sticky plates on various insects in tea gardens and to discuss the possibility for them to be applied in controlling the important pests, the luving tests, were conducted. In July when the various species of insects occurred in tea gardens, the plates were set in 0.5βhm2 tea gardens to trap the insects for 48βh, in the tea gardens of Chongqing Suburbs, in Songyang County of Zhejiang Province and in Magushan Tea Farm of Anhui Province, respectively. The results showed that the most of trapped insects belonged to Homoptera, in which the attracted amounts of harmful insects was abundant whilst the those of beneficial insects was much small, and the difference in both amounts reached the level of P<0.01. Bud green, olive yellow-green, jasmine yellow and snow white sticky plates showed much stronger trapping effect on nymphs and adults of tea green leafhoppers and adults of citrus spiny whiteflies than snow-white stiky plates. From bud green, olive yellow-green to jasmine yellow, green ingredient decreased whilst yellow ingredient increased, accordingly, the number of trapped leafhoppers decreased whilst the number of trapped whiteflies increased. It is proposed that bud green and jasmine yellow sticky plates could be used to trap the leafhoppers and the whiteflies, respectively.
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    Recent Advance on the Application of Near-infrared Spectroscopy in Tea
    ZHOU Jian, CHENG HaoP, WANG Li-yuan
    Journal of Tea Science    2008, 28 (4): 294-300.   DOI: 10.13305/j.cnki.jts.2008.04.008
    Abstract79)      PDF(pc) (383KB)(44)       Save
    The recent advance on the application of Near-infrared spectroscopy in tea was introduced in this paper. Its advantage and disadvantage in comparing with the other analytical methods were also analyzed. The main reasons which limited the development of near-infrared spectroscopic technique was analyzed and the range of the application in tea was predicted in the paper. This article also gives some advices about the development of near-infrared spectroscopy in tea.
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    The Anti-obesity Effects of Fuzhuan Brick Tea on High-fat-diet Induced Obesity in Rats
    WANG Die, HUANG Jian-an, YE Xiao-yan, CAO Dan, ZHANG Jian-wei, LIU Zhong-hua
    Journal of Tea Science    2012, 32 (1): 81-86.   DOI: 10.13305/j.cnki.jts.2012.01.007
    Abstract98)      PDF(pc) (804KB)(40)       Save
    The aim of the study was to investigate the anti-obesity effects of the Fuzhuan brick tea and green tea on a high-fat-diet induced rat model. Experimental groups were given daily oral administrations of tea aqueous extracts at different concentrations, which yields doses of 75mg/kg, 150mg/kg or 300mg/kg. The results showed that the body weight gains and the weights of perinephric fat pads and epididymal adipose tissues were significantly reduced in rats administrated with tea aqueous extracts compared with the model control group. Supplementation with tea aqueous extract decreased the values of the food utilization efficiency, fat coefficient and Lee’s index. Histological analysis in the present study showed that both tea aqueous extracts had suppressive effects on the number and size of the adipose tissue fat cells. Moreover, serum levels of the triglycerides of the Fuzhuan brick tea groups are lower than that of the green tea groups.
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    Analysis of the Aroma Components in the Pile-Fermentation of Small Leaf Tea Picked in Summer and Autumn
    JIN Dong-shuang, GONG Shu-ying, LIN Yu-hao, ZHANG Ying-bin, GU Zhi-lei, WANG Hai-jian
    Journal of Tea Science    2009, 29 (2): 111-119.   DOI: 10.13305/j.cnki.jts.2009.2.005
    Abstract93)      PDF(pc) (329KB)(38)       Save
    The pile-fermentation experiment was conducted in Zhejiang province by using the sun-dried raw green tea processed by the summer/autumn small leaf tea plant. The aroma components of the tea sampled in different pile-fermentation time were analyzed by HS-SPME/GC-MS (Headspace-Solid-phase Microextraction/Gas Chromatography-Mass Spectrometry). Resulted showed that eight groups of aroma components were identified, which were terpene and derivatives, hydrocarbons, aromatic hydrocarbons, alcohols, aldehydes, ketones, esters and acids. The main components among them were terpenes and derivatives the contents was up to 70.20% in the finished products. The maximum value of types (totally 47 kinds of compounds) of aroma was on the tenth day and the relative content was maximum (95.78%) on the fifth day after pile-fermentation. Those the components related to the sweet aroma were 3-Octanone, 3-Carene, Selinene and etc, which increasd when the sweet aroma improved. Some new components were detected in the finished products, such as Hexanal, methyl-Cyclopentane, Heptanol, 2-Pentyl-Furan, Isophoron, and others. The characteristic aroma components of the small-leaf pile-fermented tea were includeα-Cedrene, β-Cedrene, α-Gurjunene, β-Cyclocitral,β-Ionone, Cedrol, etc, especially terpene and derivatives.
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    Study and Identification on the Aroma Components of the Representative Products of Yingde Black Tea in Guangdong
    WANG Qiu-shuang, WU Hua-ling, CHEN Dong, XU Yong-quan, YIN Jun-feng, Huang Guo-zi
    Journal of Tea Science    2012, 32 (5): 448-456.   DOI: 10.13305/j.cnki.jts.2012.05.002
    Abstract101)      PDF(pc) (691KB)(55)       Save
    In the purpose of clarifying the aroma material base different from other black tea in Guangdong Yingde black tea, the determination of main aroma components was conducted, the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds in Yingde congou black tea-9, Yingde congou black tea-1 and Yingde colony black tea produced in Guangdong province, the Yunnan black tea, Indian Assam black tea and Sri Lanka UVA black tea as the control. The result showed that more than 50 aroma compounds were identified including alcohol, ester, aldehyde, ketones, alkene, hydrocarbon and etc., among which the alcohol was the most important compounds accounting for 67.03% and ester and aldehyde were next to it, accounting for 12.85% and 11.33%. Linalool, methl salicylate, linalool oxide, Nerol and nonanal were the five most important compounds, representing more than 60% of the total aroma. Flower-like and sweet aroma compounds were the main aroma character in Guangdong Yingde black tea.
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    Recognition of Wuyi Zheng Yan Tea with PLS-DA Based on Leverage Correction Using near Infrared Spectra
    ZHOU Jian, CHENG Hao, WANG Li-yuan, XUE Feng-ren, HE Xiao-yan, XU Jin-bin, HE Wei
    Journal of Tea Science    2009, 29 (1): 34-40.   DOI: 10.13305/j.cnki.jts.2009.1.006
    Abstract67)      PDF(pc) (320KB)(66)       Save
    Wuyi Yan tea was one of Olong tea which was produced only in north of Fujian province. It was a famous tea in China and the quality of Zheng Yan tea which was manufactured of tea leaves planted in the centre area of Wuyi mountain was best in Wuyi Yan tea. To recognize Zheng Yan tea , Partial least Squares Discriminant Analysis(PLS-DA)combined with leverage correction was used to discriminate Zheng Yan tea and Ban yan tea based on near-infrared spectra in this paper. The result showed that Zheng Yan tea could be recognized by PLS-DA method. 100% was correctly recognized in training set(42 samples), test set(20 samples) separately. The result proved the feasibility of discriminant recognition of Zheng Yan tea and Ban Yan tea.
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    Effects of Fluoride on the Metabolism of Tea Quality Components
    LI Qiong, RUAN Jian-yun
    Journal of Tea Science    2009, 29 (3): 207-211.   DOI: 10.13305/j.cnki.jts.2009.3.005
    Abstract79)      PDF(pc) (155KB)(37)       Save
    Soil experiment was conducted to investigate the impact of fluoride on yield of young shoots and chemical ingredients related with tea quality. Production of young shoots was improved by modest increase in the soil fluoride levels while significantly decreased by higher F levels. The concentrations of total polyphenols, caffeine and catechins (EGCG, etc) were reduced by fluoride application. The concentrations of total free amino acid and some major components (theanine, glutamate and glutamine) in the young shoots were also decreased significantly by the highest F application. Activity of phenylalanine ammonia-lyase (PAL) and glutamate synthase (GOGAT) decreased with increasing fluoride levels. The results indicated that, though tea plant growth was improved by low level of fluoride, high levels of this element were detrimental to both growth and metabolism of chemical components such as catechins, caffeine and free amino acids.
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    Status of the Total Amount of Rare Earth Oxides in Tea and Its Leaching Characteristics
    SHI Yuan-zhi, HAN Wen-yan, MA Li-feng, FANG Li
    Journal of Tea Science    2011, 31 (4): 349-354.   DOI: 10.13305/j.cnki.jts.2011.04.011
    Abstract66)      PDF(pc) (386KB)(40)       Save
    In order to figure out the status of total amount of REE oxides(∑REOs) in tea and its leaching characteristics, the statues of ∑REOs in 1245 tea samples from 2007 to 2010 were statistical analyzed, and the leaching characteristics on the different ∑REOs with different processing technology were studied. The results showed that the Median of the ∑REOs in Chinese tea was about 2mg/kg, and the ranges were from ND to 13.80mg/kg, about over 90% of tea were under 5mg/kg. The ∑REOs in over half of Oolong teas were more than 2mg/kg, but for the other tea such as green tea, scented tea, less than 30% of those teas were more than 2mg/kg. When the tea was grinded, the leaching rate of the ∑REOs was increased significantly than the original tea. And the processing technology maybe was the major factor to impact on the leaching rate of the ∑REOs. There were some rules about the leaching rate followed by low to high: needle-shaped tea< flat-shaped tea (such as Longjin tea)< Curl-shaped tea < powdered tea. The ∑REOs was highest in the first brew, and then was decreased along with the 2nd and the 3rd brews. The Powdered Oolong tea has the highest leaching rate about the ∑REOs, the leaching rate was about 60%, but even the ∑REOs in the powdered Oolong tea has reached to 6mg/kg,the ∑REOs in the brew still under the limited value about the standard in drinking water. The needle-shaped tea has the lowest leaching rate about the
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    Determination of Four Tea Catechins Content with Molecular Imprinting -SPE Extraction and Electrospray Mass Spectrometry
    GAN Ning, LI Rong-sheng, CHEN Ya-dong, XU Wei-Ming, CAO Yu-ting
    Journal of Tea Science    2009, 29 (3): 231-235.   DOI: 10.13305/j.cnki.jts.2009.3.009
    Abstract81)      PDF(pc) (250KB)(87)       Save
    A rapid method using molecular imprinted polymers (MIPs) as solid phase extraction (SPE) sorbent in pretreatment and electrospray mass spectrometry (EIMS) were investigated to separate and determine trace amount of four main catechins, such as epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGEG) respectively in tea samples. MIPs for EC (EC-MIPs) were prepared using methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EDMA) as a cross linker, and azo-bis-isobutyronitrile (AIBN) as an initiator by UV radical polymerization method; and EC-MIPs were used as SPE sorbents (EC-MIPs-SPE) for selectively trapping and preconcentrating four catechins from eluate, and were then analysed by EIMS. The results showed EC-MIPs-SPE could selectively recognize catechins. Its binding capability and selectivity for catechins were better than that of caffeine and theophylline. The optimal extraction protocol was to load samples in the aqueous phase (v/v), to wash with 50% methanol and to elute with methanol containing 1% acetatic acid. Under above conditions, the interference of caffeine was eliminated to 94.2%, and of theophylline, 100%. The relative intensity was 12.1%, 8.2%, 35.4%, and 45.7% for EC, EGC, ECG, and EGCG respectively.
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    Study on Ultrasonic Wave-Assisted Extraction of Tea
    XIAO Wen-jun, TANG He-ping, GONG Zhi-hua, XIAO Li-zheng, LI Shi, LIU Zhong-hua
    Journal of Tea Science    2006, 26 (1): 54-58.   DOI: 10.13305/j.cnki.jts.2006.01.009
    Abstract73)      PDF(pc) (324KB)(42)       Save
    The extraction effect of tea quality components by the ultrasonic wave-assisted extraction and the traditional water extraction was comparatively studied, and the best technical parameters for ultrasonic wave-assisted extraction was screened out. The results showed that tea extraction by ultrasonic wave-assisted extraction is better than that by traditional water extraction under the same technical parameters condition, which are ratio of tea to water, extraction temperature, time and times. The best operating parameters of tea extraction by ultrasonic wave-assisted extraction are 1:15 ratio of tea to water, 95℃water temperature, two times extraction (1:8+1:7) and 15βmin each time, and the extracted rate of amino acid, water soluble protein, polyphenol, water soluble carbohydrate, caffeine, simple catechin, estered catechin, water extracts can increase 23.13%、36.92%、14.88%、24.40%、19.24%、26.45%、10.12%、12.27% in sequence.
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    Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation
    GU Ji-ping, LIU Zhong-hua, HUANG Jian-an, SHI Zhao-peng
    Journal of Tea Science    2006, 26 (4): 285-290.   DOI: 10.13305/j.cnki.jts.2006.04.010
    Abstract100)      PDF(pc) (363KB)(41)       Save
    Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9βg/100βml, the concentration of enzymes is 28βg/100βml (based on the fresh weight), oxygen rate is 0.4βL/min. Temperatures and pH values in the reaction system are the most important factors(P<0.05), and adding suitable oxygen is another necessary factor in theaflavins synthesis by enzymatic oxidation.
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    Studies on Hyperlipidemia TherapyCompounds in Fuzhuan Tea
    FU Dong-he, LIU Zhong-hua, HUANG Jian-an, CAI Wen-li
    Journal of Tea Science    2012, 32 (3): 217-223.   DOI: 10.13305/j.cnki.jts.2012.03.006
    Abstract103)      PDF(pc) (700KB)(113)       Save
    Six compounds were separated from Fuzhuan Tea by using the combined modern separation technique with High-Throughput Screening(HTS) technique. After using IR,UV, MS, NMR , etc., the compounds were identified as follows: gallic acid(GA), (+)-gallocatechin 〔(+)-GC〕, 3-methoxy-4,5-dihydroxy-benzoic acid(MDBA), 3,4-dihydroxy-benzoic acid(DBA), (-)-epigallocatechin gallate〔(-)-EGCG) and (-)-epicatechin gallate((-)-ECG〕. The six compounds separated from Fuzhuan Tea were tested by cell model FXR, LXR, PPARδ, PPARγ and 3T3-L1. These results showed that the concentration of 50.00µg/mL GA and ECG were active in the FXR model. The active value was 1.77±0.14 and 3.22±0.06 separately. The active value of EGCG in the FXR model reached 6.00±0.45 when the concentration was 50.00µg/mL. To PPARδ, the active value of GC in PPARγ model was 1.62±0.16 when the concentration was 30.00µg/mL. The active value in MDBA to PPARγ was 1.73±0.16. All the compounds were not obviously active in 3T3-L1 model.
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    Study on Antitumor and Immune Regulation Activities of Anjibaicha Polysaccharide
    XIA Dao-zong, ZHANG Yuan-jun, NI Da-mei, JU Meng-ting
    Journal of Tea Science    2013, 33 (1): 40-44.   DOI: 10.13305/j.cnki.jts.2013.01.008
    Abstract88)      PDF(pc) (527KB)(37)       Save
    The antitumor activity of anjibaicha polysaccharide in vitro by MTT method was investigated by the authors. For the purpose of investigating the antitumor and immune regulation functions of anjibaicha polysaccharide in vivo, to establish the transplant tumor model of mice, and determine the inhibition rate on tumor cells growth and peritoneal macrophage phagocytic function of tumor-burdened mice by anjibaicha polysaccharide. The results showed that the sarcoma-180 growth could be significant inhibited by anjibaicha polysaccharide in vitro and showed dose-dependent relation ship. The test in vivo showed that anjibaicha polysaccharide has significant inhibitory effects on sarcoma-180 and ascitic hepatoma H22 growth in tumor-burdened mice (P<0.05). In addition, anjibaicha polysaccharide could increase thymus and spleen indexs in mice with sarcoma-180 and ascitic hepatoma H22 cells. Moreover, anjibaicha polysaccharide could significantly increase the peritoneal macrophage phagocytic function in sarcoma-180-burdened mice (P<0.05).
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    Research Progress of Cellulase and Pectinase in the Extraction of Tea Leaves
    GONG Yu-lei, WEI Chun, WANG Zhi-biao, SHEN Xue-liang
    Journal of Tea Science    2013, 33 (4): 311-321.   DOI: 10.13305/j.cnki.jts.2013.04.004
    Abstract146)      PDF(pc) (1179KB)(460)       Save
    Tea is one of the most widely consumed soft beverages. Tea beverages more excellent in flavor and formation of less deposits are more preferred. With the development of modern biotechnology, the microbial enzymes are applied in the extraction process of tea leaves. The cell walls of tea leaf and proteins are easy to be hydrolyzed, and the formation of precipitates can be avoided. The extract yields of polyphenols, thearubigins and amino acids were increased. And the tea qualities including color, odour and flavor improved accordingly. In this article, the hydrolytic enzymes including cellulase, pectinase, tannase and protease are introduced in detail, and their applications in tea extraction process are reviewed. The prospects of complex enzymes system in tea extraction process are also previewed.
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    Effects of Cadmium Stress on Growth of Tea Plant and Physiological Index in Leaves of Mengshan Tea
    XIA Jian-guo, LAN Hai-xia
    Journal of Tea Science    2008, 28 (1): 56-61.   DOI: 10.13305/j.cnki.jts.2008.01.011
    Abstract74)      PDF(pc) (422KB)(41)       Save
    Effects of different cadmium concentrations on growth of tea plant and physiological index in leaves of Mengshan tea were studied with the tea cultured in pots. Results showed that, low concentration of cadmium has no obvious adverse effect on the tea plant. When the cadmium concentration reached 60 mg/kg,the tea plant harmed to die; The chlorophyⅡ(ChⅠ) content of most spring tea supplemented with Cd was higher than CK and the increasing trend was raised firstly and inhibited later. The chlorophyⅡ content of summer tea supplemented with Cd was significantly decreased with increase of cadmium concentration, and the chlorophyⅡ content showed a negative relationship with the cadmium concentration, and the coefficient of correlation was -0.997**. The malondialdehyde (MDA) content in spring and summer tea was significantly enhanced with the increase of cadmium concentration. The coefficient of correlation were 0.970**,0.994**(P<0.01)respectively. The free proline of spring tea was significantly enhanced wiyh the increase of Cd concentration, the coefficient of correlation was 0.829*(P<0.05), the free proline in summer tea was significantly enhanced, and the coefficient of correlation was 0.980**(P<0.01),and the content of free praline in summer tea was higher than that in spring tea. The soluble sugar content in spring tea supplemented with Cd was higher than CK and the increasing trend raised firstly and then inhibited, the coefficient of correlation was -0.342;The soluble sugar content of summer tea supplemented with Cd was significantly decreased and the coefficient of correlation was -0.909*(P<0.05).
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    Requirement on Nitrogen, Phosphorus and Potassium by Tea Plants with Different Ages under Field Conditions
    YOU Xue-qin, YANG Ya-jun, RUAN Jian-yun
    Journal of Tea Science    2008, 28 (3): 207-213.   DOI: 10.13305/j.cnki.jts.2008.03.007
    Abstract55)      PDF(pc) (280KB)(23)       Save
    In order to study on requirement on nitrogen, phosphorus and potassium by tea plants with different ages under field conditions, the biomass on up-ground parts of Longjing 43 tea plants aged 1, 2, 3, 5 and 10 years in the fall of the first year, and the young shoots, the fallen leaves and the pruning on the second year under different N, P and K application was investigated. The biomass of up-ground parts in tea plants aged 5 years and the concentration of N, P and K in tea plants increased 32, 21, 25, 29 times respectively as compared with those aged one year. The biomass and the concentration of N, P and K in tea plants aged 10 years only increased approximately by 71%, 17%, 13%, 14% respectively as compared with those aged 5 years. The annual requirement of N, P and K for tea plants aged 1~2 years under field conditions is 90.01, 5.71, 31.11 kg/hm2 respectively. The annual requirement of N, P and K for tea plants aged 2~3 years is 122.74, 9.63, 45.14 kg/hm2 respectively. The annual requirement of N, P and K for tea plants aged 3~4 years is 144.03, 10.33, 47.99 kg/hm2 respectively. After pruning, the absorbed N, P and K in part is recovered to the soil through pruning and fallen leaves, the amounts of N, P and K in tea plants used for growth (pruning and fallen leaves are excluded) account for 43.9%, 48.7%, 61.3% of the total respectively. The annual requirement of N, P and K of young tea plants (for example, 5 years aged tea plants) is about 164.4, 12.68, 71.29 kg/hm2 respectively. The concentration of N, P and K in the plucking tea plants is mainly used in the growth of tea shoots, and maintain in a stable state and only share a small part of the total (approximately 7.54, 0.53 and 5.44 kg/hm2). A research method on the requirement on N, P and K of tea plants with various growing stage under field conditions was established in this investigation, thus provides a new idea for the further investigation on the nutrient requirement of tea plants.
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    Selection of Early Budding and High Quality Green-tea Cultivar
    YANG Ya-jun, YANG Su-juan, WANG Yu-shu, ZENG Jian-ming
    Journal of Tea Science    2003, 23 (s1): 9-15.   DOI: 10.13305/j.cnki.jts.2003.s1.002
    Abstract103)      PDF(pc) (311KB)(60)       Save
    :The comparison test and the reginal experiment of 6 new clones including Zhongcha 108 (RadiationII) and 4 cultivars including Zhongcha 102 were carried out. The results of the comparison test showed that Zhongcha 108 was ultra-early budding clone. The content of amino acid of Zhongcha 108 was as high as 4.53% , it was suitable for the manufacture of green-tea and the quality of made-tea was excellent. Its yield was 67.23% higher than that of Fuding Dabaicha (CK). The result of regional experiment indicated that Zhongcha102 was early budding cultivar, the content of amino acid was 4.46%, it was suitable for the manufacture of green-tea and the quality of tea was excellent, the yield was 21.68% higher than that of Yingshuang (CK). The growth rate of Zhongcha 102 is vigorous and with strong cold resistance.
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    Study on the Method of Determining Tea Color with Computer
    WANG Wen-jie, LUO Shou-jin, HUANG Jian-qin, ZHANG Li-ping, WU Xin-rong, WANG Hong-shu
    Journal of Tea Science    2005, 25 (1): 37-42.   DOI: 10.13305/j.cnki.jts.2005.01.007
    Abstract84)      PDF(pc) (277KB)(66)       Save
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    Genetic Variation in the Leaf-Expansion Period of the First Hybrid Generation Tea Plants
    GUO Ji-chun, YE Nai-xing, HE Xiao-yan
    Journal of Tea Science    2004, 24 (4): 255-259.   DOI: 10.13305/j.cnki.jts.2004.04.006
    Abstract70)      PDF(pc) (195KB)(28)       Save
    This research adopted the Huangjingui, Huangqi, Huangguanyin, Tieguanyin and Hongyafoshou as parent plants to make artificial hybridization between cultivars with various sprouting date and to observe the trend of genetic variation in the F1 leaf-expansion period. Results showed that:① The F1 leaf-expansion periods occurred a successive variation with the average value of their parent leaf-expansion periods as the central value, the variation range was large and the average value of the F1 leaf-expansion period was close to the average value of their parents. ② In the combinations of early-sprouting and early-sprouting cultivars, the super-parental phenomenon in both positive and negative directions of genetic variation of shortening or postponing the F1 leaf-expansion period were observed. In the combination of late-sprouting and early-sprouting cultivars, the F1 leaf-expansion periods were located between their parents. In the combination of late-sprouting and middle-sprouting cultivars, the F1 leaf-expansion period were mostly close to the that of middle-sprouting cultivars. ③ The male paternal showed more influence on the leaf-expansion period in F1 generation. When the leaf-expansion period of male parent was earlier, the F1 leaf-expansion period was also early in F1 generation. If the leaf-expansion period of paternal plant were same, the leaf-expansion period in F1 generation was related to the difference of the leaf-expansion period between their parents. When the difference between their parental plants was small, the large variation of leaf-expansion period occurred in the F1 generation. For these reasons, it is possible to acquire the hybrid with the earlier leaf-expansion period than their parental plants via through the hybridization between early-sprouting and early-sprouting cultivars. Simultaneously, it is possible to forecast the F1 average leaf-expansion period in the F1 generation and possible variation range in the F1 generation according to the paternal leaf-expansion period, the average value and their difference of leaf-expansion periods between the two parents.
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    Differential Proteomic Analysis in Normal Tea Shoot and Exogenous Induced Tea Shoot for Increasing EGCG Content
    LIN Jin-ke, ZHENG Jin-gui, YUAN Ming, ZHANG Xue-qin, Wang Feng
    Journal of Tea Science    2005, 25 (2): 109-115.   DOI: 10.13305/j.cnki.jts.2005.02.005
    Abstract74)      PDF(pc) (364KB)(53)       Save
    Induced by harmless inducer, the EGCG content of tea shoots improved 20.15%~25.00%. To probe into the molecular mechanism, the immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of induced tea shoots and normal tea shoots. The two- dimensional polyacrylamide gel electrophoresis map with high resolution and repeatability was produced. Differential expression analysis indicated that 14 specific proteins were emerged in induced shoot and 8 disappeared, 11 expressed above ten-fold, and 6 below one-tenth. Two differential protein spots were selected for MALDI-TOF-MS to determine the post-enzymolysis peptide mass fingerprinting. Using Mascot to search NCBInr database on website at http://www.matrixscience.com. The results were as follows: one was photosystem I iron-sulfur protein, and the another unkown. The results showed that there were proteome difference between induced and normal tea shoots, that the specific protein might play an important part during inducing process.
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    Study on the Change of the Main Biochemical Components, Representative Aromatic Compounds as well as Their Correlation of Green Tea Beverages during Storage
    DOU Hong-liang, LI Chun-mei, HAO Ju-fang, HU Wan-feng
    Journal of Tea Science    2008, 28 (3): 181-188.   DOI: 10.13305/j.cnki.jts.2008.03.001
    Abstract83)      PDF(pc) (319KB)(40)       Save
    The change of the main biochemical and aroma components of green tea beverage as well as their correction were studied. Results showed that the main biochemical components of green tea beverage changed significantly during storage. The relative concentration of total polyphenols decreased 26.6% after 12 months storage. After storing for 6 and 12 months, about 57.2% and 91.9% of EGCG was lost respectively. During storage, H2O2 was produced in the beverage, and the concentration of H2O2 changed regularly. After storage for one month, about 68% of amino acid was lost. However, it was increased after 4th month of storage. The relative contents of the representative aromatic compounds including Linalool, Linalol oxide, Geraniol, β-Lonone, Hexanal, Benzaldehyde and Nerol in the beverage increased dramatically with the storage process. By using the sas software, the correlation analysis showed that change of the main biochemical components and the change of aroma were positively correlated. The oxidation of catechin and the change of H2O2 lead to the increase in concentration of Linalool, Linalol oxide, Geraniol, β-Lonone, Benzaldehyde and Nerol. The change of amino acid and H2O2 lead to the increase of Hexanal, besides, the oxidation of catechins was confirmed as a dominant factor which lead to the change of aroma in tea beverage during storage by multiple gradually regression analysis method.
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    Determination of Trace Lead in Tea with Screen-printed Carbon Electrodes
    ZHAO Guang-ying, WU Yan-yan
    Journal of Tea Science    2008, 28 (2): 93-100.   DOI: 10.13305/j.cnki.jts.2008.02.005
    Abstract66)      PDF(pc) (537KB)(52)       Save
    The square wave anodic stripping voltammetric (SWASV) methodology using a screen printed carbon electrode (SPCE) in situ plated in mercury (Ⅱ) was developed by the authors to determine lead in tea. A detailed description of the systematic laboratory test carried out to optimize its performance is reported. A series of optimum parameter are as follows Hg2+concentration: 4×10-4 mol/L, Plating potential: -1.1 V, Estep: 3 mV, Frequency: 10 Hz, Amplitude: 60 mV, pH: 5, plating time: 280 s, balance time: 30 s. Under the optimized analytical condition, the obtained sensitivity, linearity, and detection limit are 22.7 nA/(μg/L), 10~225 μg/L (r=0.9986), and 0.74 μg/L(S/N=3),respectively. The interference effect was thoroughly studied with various metals in the determination of 120 μg/L Pb2+. Tea was digested using domestic microwave oven and PTFE sealed and pressurized microwave digestion tank. The standard method of analysis was used to detect the samples and comprised with the GB method, no significant deviation was found. The method was sensitive, accurate, fast and suitable for the determination of trace lead in tea.
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    Study on Nutritional Function of Bacterial Bio-fertilizer on Tea Plant
    ZHANG Ya-lian, CHANG Shuo-qi, LIU Hong-yan, FU Hai-ping, LI Jian-quan
    Journal of Tea Science    2008, 28 (2): 123-128.   DOI: 10.13305/j.cnki.jts.2008.02.010
    Abstract79)      PDF(pc) (204KB)(20)       Save
    The phosphorus-releasing and potassium-releasing ability of two species of bacterium in soils of tea garden (pH5.5~6.5) was investigated under laboratory condition. The amounts of bacterium per unit volume were ascertained by the absorbance of culture solution under special wavelength and various pH. Results showed the absorbance was relatively high under pH5.5~6.5, that meant these bacterium grew well under the above pH range: the bacterial bio-fertilizer made of bacterial fermentation liquid and rapeseed cake can enhance the contents of fast-acting phosphorus and potassium, improve the growth of tea shoots and increase the tea yield after applied in the soils of tea garden. Results indicated that the selected bacterial strain and the produced bio-fertilizer showed good effectiveness in proving the soil fertility and growth of tea plant and increase the yield of tea garden.
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    Study on HPLC Method for the Analysis of Trace Pigments in Ready-to-drink Tea
    PAN Shun-shun, LU Jian-liang, YANG Xiao-li, ZHENG Xin-qiang, DU Ying-ying, Devijat B, LIANG Yue-rong*
    Journal of Tea Science    2007, 27 (4): 343-348.   DOI: 10.13305/j.cnki.jts.2007.04.014
    Abstract72)      PDF(pc) (484KB)(65)       Save
    Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase. Result showed that gradient elution was suitable for analyzing by using acetonitrile/acetic acid/water in ratio of 3/0.5/96.5(v/v/v) as mobile phase A, and using acetonitrile /methanol /chloroform in ratio of 75/15/10(v/v/v) as mobile phase B. The optimized elution time program could be described that phase B increased gradiently from 80% to 100% in early 20 minutes, then remained 100% for 15 minutes. The main pigments, i.e. neoxanthin, violaxanthin, lutein, chlorophyll b, chlorophyll a, phaeophytin b, β-Carotene and phaeophytin a, could be separated upto baseline under that condition. Verification test showed that the optimized HPLC method with good repeatability and low detection limits was suitable for measurement of trace pigments in ready-to-drink tea.
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    Identification investigation of Tea Based on HSI Color Space and Figure
    WANG Jian, DU Shi-ping
    Journal of Tea Science    2008, 28 (6): 420-424.   DOI: 10.13305/j.cnki.jts.2008.06.001
    Abstract89)      PDF(pc) (527KB)(48)       Save
    The figure characteristics of tea and improved neural-network, computer vision and image processing were combined together to realize automatic identification of external quality of tea leaf. Firstly a tea-leaf image was obtained by a digital camera directly. The parameters of tea HSI model and parameters of the figure was extracted to identify tea leaf after image conversion and preprocess. Then completed automatically identify of tea-leaf through the Genetical-Neural network. The experiments reveal that the method improves the consistence between computer inspection and manual inspection.
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    Influence of the Scenting Technique Factors on Quality of Chimonanthus Tea
    PANG Xiao-li, SI Hui-qing, LI Yong-ju
    Journal of Tea Science    2007, 27 (4): 316-322.   DOI: 10.13305/j.cnki.jts.2007.04.010
    Abstract75)      PDF(pc) (638KB)(24)       Save
    For the purpose of optimizing the technological parameter for chimoncanthus tea scenting technique, the influence on quality of chimonanthus tea for the single scenting technique factors by means of Two Regression Orthogonal Combination Test was investigated. Results showed that the technique factors affected obviously on organoleptic quality of the chimonanthus tea, but not influenced on their bio-chemical composition significantly. The water content of base tea is the most remarkable among all the factors in influencing the quality of chimonathus tea, then, in proper order, the chimonanthus flower weight percentage(%), the scenting time, and the stack temperature. The optimum combination of technological parameters is: water content 10.60%, flower weight percentage 42.20%, temperature 11.3℃, and time 32.4βh.
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    Influence of Extrusion Parameters on Crude Fiber in the Extruded Green Tea Leaves
    WANG Bo, YE Yang, ZHOU Xiao-fen, QIAN Yuan-feng
    Journal of Tea Science    2012, 32 (1): 29-36.   DOI: 10.13305/j.cnki.jts.2012.01.004
    Abstract83)      PDF(pc) (588KB)(29)       Save
    Extruded green tea leave processed with a twin screw extruder, using response surface design method to establish the regression model between the moisture content of material, feeding speed, rotational speed of screw, temperature of barrel and the content of crude fiber in the green tea leaves. The results showed that the significance of parameters on the content of crude fiber in the green tea dregs was decreased according to the order of rotational speed of screw>temperature of barrel>feeding speed>moisture content of material. The optimal parameters of low content of crude fiber in the green tea dregs were as follows: moisture content of material 70%, feeding speed rotational 90r/min, speed of screw 90r/min and temperature of barrel 50℃. At this point, the content of crude fiber in the tea dregs was around 15%, compared with the raw materials before extrusion, the content of crude fiber decreased 3%.
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    Effects of Green-making Technique on Aroma Pattern of Oolong Tea
    CHEN Lin, CHEN Jian, CHEN Quanbin, ZHANG Yinggen, SONG Zhenshuo, WANG Lili, YOU Zhiming
    Journal of Tea Science    2014, 34 (4): 387-395.   DOI: 10.13305/j.cnki.jts.2014.04.011
    Abstract100)      PDF(pc) (975KB)(58)       Save
    Under controlled indoor air conditions set at (22±1)℃ with relative humidity of (71±4)%, a two-factor experiment, including degree of withering and intensity of rocking, was designed to investigate the effects of green-making technique on aroma characteristics of WIP (work in process) as compared with tea samples prepared during the respective process of Fresh-scent Oolong tea, South Fujian Oolong tea, and North Fujian Oolong tea. Aroma fingerprints of all tea samples were determined by gas chromatography coupled with mass spectrometry and processed with metabolomics analysis platform. The results from cloud plot and principal component analysis indicated that aroma pattern of WIPs would be obviously altered according to weight loss percentage of green leaves, accompanied with the increase on the contents of α-farnesene, benzeneacetaldehyde, geraniol, 3-hexen-1-ol and cis-3-hexenyl hexanoate, and the contents of phytol, indole, butylated hydroxytoluene and ethylbenzene were be decreased accordingly. However, the aromas pertinent to Oolong tea would not be fully formed only combinating on single shaking action with different intensity. Green-making was the essential procedure to bring about special aroma quality of Oolong tea, and the contents of floral scent compositions in tea samples, such as indole, trans-nerolidol, phenylacetaldehyde, etc., could be considered as the most important chemical indicators for quality control, in the green-making process of Oolong tea.
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    AFLP Analysis of Genetic Diversity of Tea plant Germplasms in Guangdong Province
    ZHAO Chao-yi, LUO Jun-wu, HUANG Jian-an, TAN He-ping
    Journal of Tea Science    2006, 26 (4): 249-252.   DOI: 10.13305/j.cnki.jts.2006.04.003
    Abstract85)      PDF(pc) (371KB)(30)       Save
    Based on AFLP method, AFLP figure were obtained in Guangdong tea plants. In this study, totally 365 bands were amplified by 5 pairs of primes and 73 bands were amplified by each primer pair on average, a total of 338 (92.6%) polymorphic AFLP markers were detected. The results indicated that the genetic distance (GD) based on AFLP data by using DPS 2000 was significant. The GD(0.5) is highest between Guibeidaye and Fenghuangshuixian, but the GD(0.12752) between Qingliangshan tea and Qingyuanbijia tea is low. This indicated that the Guangdong tea plant has a high genetic diversity.
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    Development Trends and Technical Requirements of Tea Beverage in China Mainland
    YIN Jun-feng, XU Yong-quan, YUAN Hai-bo
    Journal of Tea Science    2010, 30 (S1): 588-592.  
    Abstract80)      PDF(pc) (204KB)(85)       Save
    Through discussing the development model of beverage in the world and analyzing the development trends of tea beverage industry in China mainland, the main technical requirements for promoting development of tea beverage in China mainland are put forward.
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    Twenty Years Period in the Investigation on the Anticarcinogenic Activity of Tea
    CHEN Zong-mao
    Journal of Tea Science    2009, 29 (3): 173-190.   DOI: 10.13305/j.cnki.jts.2009.3.001
    Abstract77)      PDF(pc) (462KB)(64)       Save
    The progress in the investigation on anticarcinogenic activity of tea polyphenols in the world was analyzed and reviewed in this paper. The inhibitory activity of tea extracts on the human cancer cell was firstly reported by Fujiki H in 1987 in Japan. Since then, around 5000 papers on the anticarcinogenic activity of tea were published in the world. According to the published results of investigation, four stages were divided by the author. From the period of the end of 1980’s to the middle of 1990’s, the major investigation was focused on the in vitro and in vivo investigation and clinical investigation. From the period of the middle of 1990’s to the beginning of 21 Century, the major investigation was focused on the epidemiological investigation and the anticarcinogenic mechanism. Since the beginning of 21st Century, investigation was continuing on the anticarcinogenic mechanism on the first hand, and the metabolism and fate of catechins in the animal and human body were investigated. Since 2004, the investigation on the modification of chemical structure of tea catechins was conducted for the purpose of improving the bioavailability, stability and bioactivity of tea catechins in human body. This paper is mainly reviewed from the published literature after 2003 from the following five parts: results on the epidemiological investigations, anticarcinogenic mechanism, the active components of anticarcinogenic activity in tea, results on the metabolism and fate of tea catechins in animal and human body, modification of chemical structure of tea catechins and its bioavailability and bioactivity.
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    Construction of No-Pollution Control System on Tea Pests Based on Chemical Ecology
    SUN Xiao-ling, CHEN Zong-mao
    Journal of Tea Science    2009, 29 (2): 136-143.   DOI: 10.13305/j.cnki.jts.2009.2.009
    Abstract96)      PDF(pc) (270KB)(45)       Save
    Progress and application of ecological functions of the sex pheromone of tea pests, herbivore induced tea volatiles, extraneous induction on resistance of tea plant, and behavioral regulation effect of secondary metabolites from nonhost on Ectropis obliqua Prout and Empoasca vitis (Göthe) were introduced. The construction and prospects on no-pollution control system of tea pests based on the current chemical ecology achievements was discussed.
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    Researches on Tender Tea Shoots Identification under Natural Conditions
    WEI Jia-jia, CHEN Yong, JIN Xiao-jun, ZHENG Jia-qiang, SHI Yuan-zhi, ZHANG Hao
    Journal of Tea Science    2012, 32 (5): 377-381.   DOI: 10.13305/j.cnki.jts.2012.05.004
    Abstract73)      PDF(pc) (772KB)(42)       Save
    Identification of the tender tea shoots is the key step towards the intelligent tea harvesting. This paper presents several methods to recognize the tender tea shoots for high-quality tea production. Gray images were obtained by five color indices, which were R-B, I, b, S and Cb in RGB, YIQ, Lab, HSI and YCrCb color spaces. Then suitable threshold methods were applied to segment image, finally the median filter was used to eliminate noises. The results indicate that these methods were particularly effective for tender tea shoots identification under their natural conditions. The proposed method can be used for future intelligent tea harvest development.
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    HPLC-ESI-MS Analysis of Pigments in ZijuanGreen Tea and Its Stability
    GONG Jia-shun, SUI Hua-song, PENG Chun-xiu, FAN Jian-gong, LI Ya-li
    Journal of Tea Science    2012, 32 (2): 179-188.   DOI: 10.13305/j.cnki.jts.2012.02.006
    Abstract80)      PDF(pc) (843KB)(24)       Save
    The pigments and its stability in Zijuan sun-dried green tea were investigated in this study. The pigments from Zijuan sun-dried green tea were extracted by acidic methanol-H2O solution and H2O, and the main compounds were identified by means of HPLC-ESI-MS/MS techniques. 14 anthocyanin, 24 flavonols, 8 phenolics and theaflavins and 3 alkaloids were identified from the extracts of Zijuan sun-dried green tea. The main anthocyanins, such as Cyanidin-3-glucoside, Cyaniding-3-galactoside, Cyanidin-3-O-(6-p-coumaroyl)-glucoside, Delphinidin-3- glucoside, Delphinidin-3-rutinoside, Delphinidin-3-O-(6-p-coumaroyl)-glucoside, Prodelphinidin B-2(or 4)3′-O- gallate, Procyanidin B-2(or 4)3′-O-gallate, Petunidin-3-glc-4-vinylphenol and Malvidin-3-glc-4- vinylepicatechin etc were identified in Zijuan sun-dried green tea. The pigment extracted from Zijuan sun-dried green tea by using acidic methanol-methanol-H2O as solvent showed good water-solubility and was stable to light and heat in acid environment, but sensitive to the pH value of environment, oxidizer and reducer. The maximum adsorption wavelength for the pigment is 538nm under pH 1.0. Fe2+ and Cu2+ can change the color of the pigments and stability was decreased. The substance of the purple colour in Zijuan sun-dried green tea was clarified by HPLC-ESI-MS/MS analysis.
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    Molecular Identification, Bioinformatic Analysis and Prokaryotic Expression of the Cyclophilin Gene Full-length cDNA from Tea Plant (Camellia sinensis)
    ZHANG Ya-li, ZHAO Li-ping, MA Chun-lei, CHEN Liang
    Journal of Tea Science    2007, 27 (2): 120-126.   DOI: 10.13305/j.cnki.jts.2007.02.005
    Abstract74)      PDF(pc) (1813KB)(35)       Save
    A cDNA clone, encoding cyclophilin, obtained by random sequencing of young shoot cDNA library from tea plant (Camellia sinensis). The full-length cDNA of the cyclophilin gene was 949 bp(GenBank accession No. DQ904327), containing a putative ORF of 495 bp, encoding 164 amino acids and the predicted MW were 17.47 kD and pI was 8.54, respectively. A “CAAT” signal in 5′ untranslated region and a polyadenylated signal of “AATAA”poly-A in 3′untranslated regions of cyclophilin mRNAs were found. The presumed amino acid sequences of tea plant were aligned with those of other 26 organisms through CLUSTAL W. The phylogenetic analysis based on the Neighbor-Joining method showed the similarity was greater than 85% between cyclophilin genes of tea plant and Oryza sativa (japonica cultivar-group), Solanum tuberosum, Triticum aestivum etc. Primers were designed on the open reading frame of the cyclephilin gene of tea plant to construct the expressive vector pET/Csin-Cyp. A recombinant protein about 23 kD in the Escherichia coli BL21 (DE3) was induced.
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    Pharmacokinetics and Bioavailability of Catechin Liposome in Rabbits
    SUN Zhi-liang, Liu Xiang-xin, Ling Qin-lu, Liu Zi-kui, Liu jin-fei, He Xiang rong
    Journal of Tea Science    2004, 24 (1): 44-48.   DOI: 10.13305/j.cnki.jts.2004.01.009
    Abstract89)      PDF(pc) (299KB)(98)       Save
    Pharmacokinetics and bioavailability of Catechin Liposome were studied in 10 healthy rabbits following single intravenous (25mg/kg) and oral administration (25mg/kg)of the drug. Plasma concentrations of. Catechin Liposome were determined by high-performance liquid chromatography. The concentration-time data were fitted to a two-compartment model following a single intravenous injection in rabbits. The main pharmacokinetic parameters were as follows: distribution half-life (t1/2α) (0.18±0.01)h,elimination half-life (t1/2β) (1.52±0.08)h,apparent distribution volume (Vd) (4.48±0.24)L total body clearance (ClB) (2.05±0.07)L/h,the area under curve (AUC) (29.20±1.00)mg/(L.h). The drug concentration-time data also were fitted to a one-compartment open model with first order absorption after a single oral administration Catechin Liposome. The pharmacokinetic parameters were as follows: t1/2kα (0.27±0.03)h, t1/2ke(1.72±0.04)h, tmax (0.87±0.05) h, Cmax 6.53±0.62βmg/L, AUC 25.90±1.34βmg/(L.h), bioavailability F 88.60±5.73%. Pharmacokinetic characteristics of Catechin Liposome in healthy rabbits showed a rapid absorption, the time to reach Cmax is short, slow elimination, long half-life, large apparent distribution volume, and high bioavailability of oral administration. The results showed:after Catechin was prepared in liposome form, the tissue distribution and pharmacokinetics were changed significantly.
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    Study on Separation and Concentration of Theanine in De-catechined Tea Extracts by Membrane System
    XIAO Li-zheng, XIAO Wen-jun, GONG Zhi-hua, WANG Wei, LIU Zhong-hua
    Journal of Tea Science    2006, 26 (1): 37-41.   DOI: 10.13305/j.cnki.jts.2006.01.006
    Abstract71)      PDF(pc) (324KB)(40)       Save
    :By using the material of de-catechined tea extracts in tea comprehensive processing and on the basis of selecting the best pH value of liquid for separation and concentration theanine by ultra-filtration,the effect on the yield rate and purity of theanine by 2500βDa、3500βDa、5000βDa ultra-filtration and four concentrating method of 300βDa nano-filtration、200βDa nano-filtration、reverse osmosis、vacuum vaporation concentration were comparatively studied in processing the de-catechined tea extracts. The results indicated that it was beneficial to separate and concentrate theanine from de-catechined tea extracts when the value of pH was 2.8~3.5.3500βDa ultrafiltration had a good effect in separating and concentrating theanine ,the major tea polyphenol ,water soluble carbohydrate were preserved and the cut rate reached 89.90%、92.20% respectively, the yield rate and purity of theanine was 54.50%,8.92% respectuvely. The lost rate of theanine in concentrating of 300βDa nano-filtration、200βDa nano-filtration、reverse osmosis、vacuum vaporation concentration was 4.51%、3.62%、0.45%、5.15% respectively. Take all thing into account, it is feasible to separate and concentrate theanine from de-catechined tea extracts in tea comprehensive processing industry by the joint application of ultra-filtration and reverse osmosis,and the yield rate and purity of theanine can reach 54.05% and 8.53% respectively.
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    Advances in Tea Plant Genetics and Breeding
    LIANG Yuerong, SHI Meng
    Journal of Tea Science    2015, 35 (2): 103-109.   DOI: 10.13305/j.cnki.jts.2015.02.001
    Abstract103)      PDF(pc) (567KB)(81)       Save
    Advances in germplasm enhancement of tea breeding, techniques for identification of new tea cultivars, changes in breeding objectives, improvement of breeding program, achievements of tea breeding, system of propagation and extension of new tea cultivars were reviewed in this paper, which provides useful information for further studies in tea genetics and breeding. Hybridization is still the major method for innovating tea breeding materials, and the physical and chemical mutagenesis methods have been extensively used, while transgenic technique remains to be further improved. Combination of the high resolution inspecting equipments and the gene identification technology with the forecasting models established by computer technology made the early identification of tea cultivars more accurate. The breeding target has been diversified after going through yield breeding, quality breeding and early flushing breeding stages. Improving early identification accuracy and shortening breeding cycle will be the aim for tea breeding technology development.
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    Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain
    MAO Qing-li, SHI Zhao-peng, LI Lin, LIU Zhong-hua, ZHU Qi
    Journal of Tea Science    2007, 27 (3): 243-247.   DOI: 10.13305/j.cnki.jts.2007.03.006
    Abstract96)      PDF(pc) (495KB)(26)       Save
    This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.The results showed that the catechin (TC80), which MIC90 was 25~100 μg/ml, showed the obvious inhibition of Agrobacterium rhizogenes. This showed that the plenty of polyphenols in the tea plant was a key factor to restrict Agrobacterium rhizogenes-mediated transformation of tea plant. An anti-polyphenol strain R1000AP obtained by anti-polyphenol screening, which anti-polyphenol capability enhanced 10~40 times. The hairy root frequency induced by the strain increased by 46.5%(P<0.05)compared to the non-anti-polyphenol strain.
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    Positive Studies on Characteristics of Tea Consumers in Hangzhou City
    TANG Yi, HUANG Han-dan
    Journal of Tea Science    2007, 27 (4): 323-327.   DOI: 10.13305/j.cnki.jts.2007.04.011
    Abstract95)      PDF(pc) (391KB)(91)       Save
    According to the results of questionnaire survey in Hangzhou city, Ordinal Logit model was applicated to explain relations between characteristics of consumers and level of tea demand. The regression results showed: custom of tea drinking showed the most important impact on tea consumption amount of Hangzhou citizen, moreover the major factor to affect purchasing price of tea is level of income. Other relevant factors include age, degree of trust in tea’s efficacy, price sensitivity and so on, whereas gender, educational background and occupation showed no obvious effect on tea demand.
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    Study on the Method of Fetching Unbroken Tea Color with Computer
    LI Jie, QI Gui-nian
    Journal of Tea Science    2007, 27 (4): 328-334.   DOI: 10.13305/j.cnki.jts.2007.04.012
    Abstract117)      PDF(pc) (315KB)(42)       Save
    A new method of fetching unbroken tea color with computer,named L Method,has been established. The accuracy of the method was verified,and the further investigation also has been fulfilled. The results showed that the L method can fetch the color of tea accurately and quickly. Scanned with resolution 100βdpi,the credible interval(95%) of the result is: L=0,-0.45≤a≤0.45,-0.71≤b≤0.71. When the scanned area is 11βcm×11βcm or more,the color parameters of tea scanning photo may fully stand for the tea actual color. The SE is: -0.16≤L≤0.16,-0.13≤a≤0.13,b=0. There was obvious distinction between the color of unbroken tea and crush tea, so the later can't represent the tea actual color. Using computer to fetch color parameters is better than chroma meter.
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    The Detection Method of Nitrite in Tea
    GU Xiao-ling, LU Cheng-yin*, LIU xin, YU Liang-zi, CHEN-Li-yan, WANG-Qing-hua
    Journal of Tea Science    2007, 27 (2): 159-162.   DOI: 10.13305/j.cnki.jts.2007.02.011
    Abstract67)      PDF(pc) (379KB)(29)       Save
    A spectophotometric method on the determination of nitrite in tea was investigated, which is based on the diazotization of nitrite with p-aminobenzene sulfonic acid under acidic conditions then coupled with N-(1-Naphthyl)- ethylenediamine dihydrochloride in forming the colour-producing response. Because of the interference of polyphenols, pigment and ascorbic acid in tea, the optimum operating conditions of measurement was established. The method showed a good analytical characteristics with correlation coefficients of 0.9993, variation coefficient of 4.6%, and recovery was ranging from 72.90% to 86.53%. It is a rapid, accurate, simple method and applicable for the determination of nitrite in tea.
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    Studies on the Extraction of Tea-leaf Protein
    CAI Zhi-ning, WANG Chun-yan, LIANG Cui-jin, LI Qing-yu
    Journal of Tea Science    2008, 28 (4): 309-312.   DOI: 10.13305/j.cnki.jts.2008.04.001
    Abstract78)      PDF(pc) (278KB)(38)       Save
    :The investigation was designed by using these reference factor including the solid to liquid ratio, extraction time, pH value and extraction temperature, as to determine the optimum technological parameters of tea-leaf protein extraction process and the optimum combination. Results showed that the optimum condition is as follows: solid to liquid ratio of 1:8, pH11, 60℃, 60 min. The protein extraction rate was 21.89% under these conditions. The extraction process of tea protein and the equipment used are simple. The optimal pH of sedimentation is pH 4.0.
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    The Regulation of Oolong Herb Tea on the Serum Lipid Level and the Preventive Effects of ECV in Experimental Hyperlipidemia Rats
    ZHENG Liang-pu, LIN Wei, YE Hong-zhi, CHEN Wen-lie, HU Hai-xia
    Journal of Tea Science    2008, 28 (2): 107-110.   DOI: 10.13305/j.cnki.jts.2008.02.012
    Abstract61)      PDF(pc) (216KB)(49)       Save
    To establish the hyperlipidmia rat models by feeding high lipid diet, then the herb, tea and oolong herb tea were irrigated into stomach separately for 35 days in succession, the level on the activity of ALT, AST enzyme and the level of cholesterols of TCHO, TG, HDL-C, LDL-C, as well the contents of NO and ET-1 in rat blood measured were measured. Meanwhile, the general conditions and histopathological changes in tissues of liver and kidney were observed to explore the influence of Oolong herb tea on the serum lipid level and the preventive effect on vein endothelium cells in experimental hyperlipidemia rats investigated. Results showed that compared the blood levels of TCHO, TC, LDL-C, TCHO and the ET-1 content were reduced significantly in treatment group while HDL-C and NO increased(P<0.05, P<0.01) compared with the control group. The effect of Oolong herb tea was more remarkable than that of herb and tea treatment. It is concluded that the herb, tea and Oolong herb tea are able to regulate the level of serum lipid effectively prevent hyperlipidemia and have a protective effect of vein endothelium cells. It is showed that the synergistic effect between herb and tea and improve the efficacy in Oolong herb tea.
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    Study on the GCG in Green Tea
    LU Hai-peng, TAN Jun-feng, GUO Li, GU Ji-ping, LIN Zhi
    Journal of Tea Science    2008, 28 (2): 79-82.   DOI: 10.13305/j.cnki.jts.2008.02.002
    Abstract101)      PDF(pc) (246KB)(40)       Save
    The GCG content in different tea germplasms, change of GCG content during green tea processing and the tea leaf treated with high temperature steam were studied by using the HPLC determination. Results showed that the content of GCG in five tested tea cultivars was more than 1.5% among 200 tea germplasms, and it was increased continuously during the green tea processing. Temperature was the key factor that influenced the GCG content in tea, and the high temperature steam treatment could effectively increase the GCG content in green tea.
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    Study on the Burning Character of Tea-seed Oil as An Alternative Fuel in Diesel Engine
    MA Rong-chao, QIN Wen
    Journal of Tea Science    2004, 24 (2): 105-108.   DOI: 10.13305/j.cnki.jts.2004.02.006
    Abstract74)      PDF(pc) (312KB)(46)       Save
    Vegetal energy is becoming one of the important energy sources for diesel engine. A test of burning character on S195 diesel engine was carried out using tea-seed oil as the alternative fuel. The results showed that it is feasible to burn mixture oil of tea-seed oil and diesel oil without changing the structure of the engine. When burning the tea-seed oil or mixture fuel in the engine, the exhaust pollution is on decline, but the specific fuel consumption rats and deposit charcoal of combustion chambers and injection nozzles are more than ever. The performance of the engine is rather good when burning the mixture of tea-seed oil and diesel oil in same volume and the angle of pre-oil-supplied is 19o CA.
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    Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice
    PIAO Zai-ri, Wang Yue-fei, YANG Xian-qiang, CHEN Liu-ji, YANG Yong, ZHAO Bao-lu, JIAO Hong-li, ZHOU Dang-yi
    Journal of Tea Science    2003, 23 (2): 119-123.   DOI: 10.13305/j.cnki.jts.2003.02.007
    Abstract75)      PDF(pc) (304KB)(146)       Save
    The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.
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    Cited: Baidu(8)
    Effect of Heat Sterilization and Storage on Quality Attributes of Fresh Green Tea Beverages
    ZHANG Ling-yun, LIANG Yue-rong, SUN Qi-fu, SUN Qing-lei, LU Jian-liang, E.G. Mamati
    Journal of Tea Science    2003, 23 (2): 171-176.   DOI: 10.13305/j.cnki.jts.2003.02.017
    Abstract65)      PDF(pc) (356KB)(46)       Save
    Effect of heat sterilization and storage on quality attributes of fresh green tea beverages was studied. The results showed that the lightness and greenness of the infusions decreased with the treatments of heat sterilization and storage whereas the yellowness increased. The concentration of tea polyphenols and amino acids did not correspondingly change with the colors, but the concentration of caffeine in the beverage decreased during storage. Among the major catechins, EGCG、ECG、EGC decreased, and GCG、CG、GC increased with heat sterilization and storage treatments. The changes in quality attributes of the different beverages could be due to variation in content of tea catechins, amino acid and caffeine.
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    Isolation and Molecular Identification of Fungal Colonization on LiuPao Tea in Guangxi
    MAO Yan, HUANG Li, WEI Bao-yao, XIA Ning, TENG Jian-wen
    Journal of Tea Science    2013, 33 (6): 556-561.   DOI: 10.13305/j.cnki.jts.2013.06.003
    Abstract100)      PDF(pc) (699KB)(36)       Save
    A strain of “Jinhua” fungi was isolated from Liupao tea which manufactured Guangxi Yintai Tea Factory in 2008 (No. 6918). The morphology of colony of the tested fungi was observed with microscope, and the tested fungi was identified in the molecular level by DNA sequencing. According to the characteristic of the morphology, microscopic as well as the DNA sequencing of the tested strains, it was identified as Eurotium niveoglaucum.
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    Effects of Different Biomass Materials on the Amelioration of Acidic Tea Garden Soil
    XIE Shao-hua, ZONG Liang-gang, CHU Hui, WANG Zhang-yi, QIU Xiao-lei, MA Ai-jun, HE Ren-hong
    Journal of Tea Science    2013, 33 (3): 279-288.   DOI: 10.13305/j.cnki.jts.2013.03.009
    Abstract85)      PDF(pc) (758KB)(32)       Save
    The amelioration effects of different biomass materials applied to three acidic tea garden soils were examined with the incubation experiments. The results indicated that the pH of three soils (LC, LG and LX) increased by 0.44, 0.31 and 0.26 units averagely while the contents of soil exchangeable acidity, exchangeable aluminum, total soluble aluminum, and total monomeric aluminum decreased significantly after biomass materials treatment for 45 days. Among different biomass materials, the amelioration effects of cow dung, straw compost and commercial organic fertilizer were more prominent than the others. The straw carbon was more effective than other materials in increasing the exchangeable cation contents and the degree of soil base saturation, as well as the soil cation exchange capacity and soil organic matter contents. These results help to better understand the mechanisms of the amelioration.
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    Influence of AM on the Growth of Tea Plant and Tea Quality under Salt Stress
    LIU Jie, XIAO Bin, WANG Li-xia, LI Jiao, PU Guo-tao, GAO Ting, LIU Wen
    Journal of Tea Science    2013, 33 (2): 140-146.   DOI: 10.13305/j.cnki.jts.2013.02.012
    Abstract84)      PDF(pc) (628KB)(57)       <