茯砖茶不同萃取物对消化酶活性的影响

doi:10.13305/j.cnki.jts.2008.01.003

茶叶科学 ›› 2008, Vol. 28 ›› Issue (1): 62-66.doi: 10.13305/j.cnki.jts.2008.01.003

茯砖茶不同萃取物对消化酶活性的影响

傅冬和^1,2, 刘仲华^1,2,*, 黄建安^1,2, 陈惠衡²

1. 湖南农业大学教育部茶学重点实验室,湖南长沙 410128;
2. 湖南农业大学园艺园林学院,湖南长沙 410128

收稿日期:2007-07-20 修回日期:2007-09-17 出版日期:2008-02-15 发布日期:2019-09-16
通讯作者: *larkin-liu@163.com
作者简介:傅冬和(1967— ),女,湖南醴陵人,副教授,博士,主要研究方向植物功能成分研究与开发。
基金资助:
科技部十五重大科技攻关项目(2004BA542C)及湖南省教育厅基金（06C404）

The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme

FU Dong-he^1,2, LIU Zhong-hua^1,2,*, HUANG Jian-an^1,2, CHEN Hui-heng²

1. Tea Key Lab of the Ministry of National Teaching, Changsha 410128, China;
2. College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China

Received:2007-07-20 Revised:2007-09-17 Online:2008-02-15 Published:2019-09-16

摘要/Abstract

摘要： 将茯砖茶依次用三氯甲烷、乙酸乙酯及正丁醇萃取后制备样品,测定各部分样品对α-淀粉酶及胰脂肪酶活性的影响。研究发现茯砖茶各部分样品对胰脂肪酶活性的影响程度不同,三氯甲烷层稍有抑制作用,活性只有对照的0.82倍,水提物、乙酸乙酯萃取层及正丁醇萃取层有显著的激活作用,正丁醇萃取层部分激活作用最明显,达2.54倍。茯砖茶各部分样品对α-淀粉酶活性均有激活作用,其中以乙酸乙酯萃取层部位的激活作用最强,激活倍数为4.09,其次是水提物,其激活倍数为2.99,水层的激活作用不显著。三氯甲烷萃取层抑制作用不显著,活性只有对照的0.88倍。乙酸乙酯萃取层及正丁醇萃取层部分经过HP-20树脂再分离后,各部分仍有较高的活性。

关键词: 茯砖茶, α-淀粉酶, 胰脂肪酶, 酶活性, 内含成分

Abstract: Fuzhuan Tea was extracted by chloroform, ethyl acetate, 1-butanol in turn. The effect of Fuzhuan Tea on the activities of metabolize enzyme was investigated. The results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity. The sample extracted by chloroform could restrain pancreatic lipase a little and the relative activity was 0.82. The water extracts, the sample extracted by ethyl acetate and 1-butanol showed obvious activation, that of the sample extracted by 1-butanol was the most active. The multiple was 2.54. All fractions of Fuzhuan Tea were active to α-amylase, among them, the sample extracted by ethyl acetate was the most active. The relative activiy was 4.09. The water extracts was the second one and its relative activity was 2.99. That of the layer of water sample was not obvious. The sample extracted by chloroform could restrain α-amylase a little and the relative activity was 0.88. The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to α-amylase and pancreatic lipase respectively.

Key words: Fuzhuan Tea, α-amylase, pancreatic lipase, enzyme activity, component

中图分类号:

傅冬和, 刘仲华, 黄建安, 陈惠衡. 茯砖茶不同萃取物对消化酶活性的影响[J]. 茶叶科学, 2008, 28(1): 62-66. doi: 10.13305/j.cnki.jts.2008.01.003.

FU Dong-he, LIU Zhong-hua, HUANG Jian-an, CHEN Hui-heng. The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme[J]. Journal of Tea Science, 2008, 28(1): 62-66. doi: 10.13305/j.cnki.jts.2008.01.003.

参考文献

[1] 屠幼英, 须海荣, 梁惠玲, 等. 紧压茶对胰酶活性和肠道有益菌的作用[J]. 食品科学, 2002, 23(10): 113~116.
[2] 吴媛媛, 屠幼英, 陈文峰, 等. 紧压茶对α-淀粉酶促活作用的研究[J]. 中国茶叶加工, 2002, 16(1): 38~39.
[3] 张盛, 刘仲华, 黄建安, 等. 吸附树脂法制备高纯儿茶素的研究[J]. 茶叶科学. 2002, 22(2): 125~130.
[4] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶学科学, 2004, 24(1): 53~59.
[5] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1995.
[6] The Amylase Research Society of J apan (Ed). Handbook of Amylase and Related Enzymes[M]. Oxford: Oxford Pergamon Press, 1988: 53~56.
[7] 周景祥, 王桂芹, 余涛. 蛋白酶和淀粉酶活性检测方法探讨[J]. 中国饲料, 2001, 11: 23~24.
[8] 曹建明, 谢作叙, 周铁丽. 改良的淀粉酶测定法[J]. 陕西医学检验, 2001, 16(3): 63.
[9] Gotz F, Poop F, Schleifer K H.Complete nucleotide sequence of the lipase gene from staphylococcus hyicus cloned in staphylococcus hyicus[J].Nucleic Acid Res., 1985, 13: 5895~5906.
[10] 屠幼英, 梁慧玲, 陈暄, 等. 紧压茶儿茶素和有机酸的组成分析[J]. 茶叶, 2002, 28(1): 22~24.
[11] Datta S, Lou C, Li W, et a1.Human hepaticlipase[J]. J Biol Chem, 1988, 263: 1107~1110.
[12] Winkler F K, D'Arcy A, Hunzuker W. Structure of human pancreatic lipase[J]. Nature, 1990, 343: 771~774.
[13] Huang A H C.In Lipases (Borgströn B and Brockman H L, eds) Elsevier[M]. Amsterdam, 1984: 365~416.
[14] Cardenas J, Alvarez E,de Castro-Alvarez M-S, et a1.Screening and catalytic activity in organlc synthesis of novel fungal and yeast lipase[J]. J Mol Catal B, 2001, 14: 111~123.
[15] Osada K, Takahashi K, Hatano M.Polyunsaturated fatty acid glyceride syntheses by microbial lipase[J]. J Am 0il Chem Soc, 1990, 67: 921~922.
[16] Kaimal T N B, Saroja M. Enhancement of cata1ytic activity of porcine pancreatic lipase by reductive alkylation[J]. Biotechno1 lett, 1989, 11: 31.

茯砖茶不同萃取物对消化酶活性的影响

The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme

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