L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究

doi:10.13305/j.cnki.jts.2016.05.004

茶叶科学 ›› 2016, Vol. 36 ›› Issue (5): 469-476.doi: 10.13305/j.cnki.jts.2016.05.004

L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究

刘遵莹^1,2, 刘秋玲^1,2, 邓燕莉^1,2, 陈凌^1,2, 肖文军^1,2,3,*

1. 湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2. 湖南农业大学国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
3. 湖南农业大学湖南省植物功能成分利用协同创新中心,湖南长沙 410128

收稿日期:2016-03-24 出版日期:2016-10-15 发布日期:2019-08-26
通讯作者: *,xiaowenjun88@sina.com
作者简介:刘遵莹,女,硕士研究生,主要从事茶叶功能成分利用研究。
基金资助:
国家“十二五”科技支撑计划项目（2011BAD10B00）

Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC

LIU Zunying^1,2, LIU Qiuling^1,2, DENG Yanli^1,2, CHEN Ling^1,2, XIAO Wenjun^1,2,3,*

1. Key Lab of Tea Science of Ministry of Education, Changsha 410128, China;
2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China

Received:2016-03-24 Online:2016-10-15 Published:2019-08-26

摘要/Abstract

摘要： 以SPF级Balb/c雌性小鼠为实验动物,对其适应性饲养3 d后连续30 d灌喂不同剂量L-茶氨酸,然后腹腔注射产肠毒素大肠杆菌E44813诱导免疫应激,5 h后取样,分析研究了L-茶氨酸对小鼠体重,回肠组织谷胱甘肽过氧化物酶（Glutathione peroxidase, GSH-Px）、过氧化氢酶（Homogenate catalase, CAT）与诱导型一氧化氮合成酶（Inducible nitric oxide synthase, iNOS）活性,丙二醛（Malondialdehyde, MDA）含量、空肠组织病理学变化,以及血清肿瘤坏死因子α （Tumor necrosis factor-α, TNF-α）、细胞间粘附分子1（Intercellular cell adhesion molecule-1, ICAM-1）和白介素1β（Interleukin-1β, IL-1β）表达量的影响,以探明L-茶氨酸对产肠毒素大肠杆菌免疫应激小鼠肠道的保护作用。结果表明,各剂量L-茶氨酸均能显著增加小鼠体重,可减轻产肠毒素大肠杆菌E44813引起的肠道组织病变程度,升高回肠组织GSH-Px和CAT酶活性,降低MDA含量和iNOS酶活性,在不同程度上抑制血清TNF-α、IL-1β和ICAM-1的表达量。说明L-茶氨酸可通过抑制炎性细胞因子表达水平、降低脂质过氧化程度和提高抗氧化能力来维护肠道组织形态与结构的完整性,并达到保护肠道的作用。

关键词: L-茶氨酸, 产肠毒素大肠杆菌, 免疫应激, 保护肠道

Abstract: SPF grade Balb/c female mice were used as experimental animals. After adaptive feeding for 3 days, mice were orally pre-treated with different dosages of L-theanine once daily for 30 days before ETEC E44813 intraperitoneal injection. The body weight change in mice was analyzed during the breeding process. Mice were scarified 5 hours after ETEC injection and tissues were collected for analysis. The activities of ileum Glutathione peroxidase (GSH-Px), homogenate Catalase (CAT), Malondialdehyde (MDA) and Inducible nitric oxide synthase (iNOS) were measured and the pathological changes of intestinal and the levels of serum Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β) and Intercellular cell adhesion molecule-1(ICAM-1) were examined. This study was

Key words: L-theanine, Enterotoxigenic Escherichia coli., immune stress, intestinal protection

中图分类号:

Q51
Q939.1

刘遵莹, 刘秋玲, 邓燕莉, 陈凌, 肖文军. L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究[J]. 茶叶科学, 2016, 36(5): 469-476. doi: 10.13305/j.cnki.jts.2016.05.004.

LIU Zunying, LIU Qiuling, DENG Yanli, CHEN Ling, XIAO Wenjun. Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC[J]. Journal of Tea Science, 2016, 36(5): 469-476. doi: 10.13305/j.cnki.jts.2016.05.004.

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L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究

Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC

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