一株分离自茶轮斑病病叶的病原物鉴定

doi:10.13305/j.cnki.jts.2026.02.001

摘要/Abstract

摘要： 茶轮斑病是我国茶区重要病害之一,时常见到在茶树老叶、成叶、幼嫩芽叶上发生。从贵州都匀毛尖产茶区茶轮斑病罹病茶树叶片上分离纯化出一个菌株L352,根据形态学结合ITS、β-tub和EF-1α基因构建的多基因串联系统发育树,对其进行种类鉴定。结果表明,菌株L352分生孢子大部分为5个细胞4个横隔膜,中间3个有色细胞;偶有发现6个细胞5个横隔膜,中间有4个色细胞;系统发育树显示其与Pestalotiopsis rosea聚集成一个相对独立的进化枝,支持率为100%,最终确定该病原菌为P. rosea,并首次发现该菌种对茶树致病。

关键词: 茶轮斑病, 拟盘多毛孢, 玫瑰拟盘多毛孢, 鉴定

Abstract: Tea grey blight is one of the most important diseases in tea producing areas in China, which is common in old leaves, mature leaves and young bud leaves. In this study, a strain L352 was isolated and purified from tea grey blight leaves in Duyun City, Guizhou. The strain L352 was identified based on morphological characteristics and a multi-gene phylogenetic tree was established using the ITS, β-tub and EF-1α genes. The results show that the conidia of strain L352 were mostly composed of 5 cells with 4 transverse-septa, featuring three pigmented cells in the middle. Occasionally, 6 cells with 5 transverse-septa were observed, containing four pigmented cells in the middle. The phylogenetic tree indicates that it was clustered into a relatively independent clade with Pestalotiopsis rosea, with a support rate of 100%. The pathogen was finally identified as Pestalotiopsis rosea, and it was the first discovery of this species causing tea grey blight disease in Camellia sinensis.

Key words: tea grey blight, Pestalotiopsis, Pestalotiopsis rosea, identification

中图分类号:

刘荣, 姚玉仙, 王兴娥, 刘冰翔, 帅琴, 赵永田. 一株分离自茶轮斑病病叶的病原物鉴定[J]. 茶叶科学, 2026, 46(2): 311-317. doi: 10.13305/j.cnki.jts.2026.02.001.

LIU Rong, YAO Yuxian, WANG Xing′e, LIU Bingxiang, SHUAI Qin, ZHAO Yongtian. Identification of A Pathogen Isolated from Tea Grey Blight Leaves[J]. Journal of Tea Science, 2026, 46(2): 311-317. doi: 10.13305/j.cnki.jts.2026.02.001.

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