等离子活化乳酸钠增强幼龄茶树次生代谢及生理抗性

茶叶科学 ›› 2026, Vol. 46 ›› Issue (1): 61-72.

等离子活化乳酸钠增强幼龄茶树次生代谢及生理抗性

张云帆^1,2, 周凤珏², 胡钧铭^1,*, 宋传奎³, 郑富海¹, 张俊辉¹, 李婷婷¹, 李宇翔¹

1.广西农业科学院农业资源与环境研究所/广西耕地保育重点实验室,广西南宁 530007;
2.广西大学农学院,广西南宁 530004;
3.安徽农业大学茶业学院,安徽合肥 230036

收稿日期:2025-06-07 修回日期:2025-09-04 出版日期:2026-02-15 发布日期:2026-02-06
通讯作者: * jmhu06@126.com
作者简介:张云帆,男,硕士研究生,主要从事茶树抗逆生理及品质形成方面的研究。
基金资助:
广西科技基地和人才专项（桂科AA23026002）; 中央引导地方科技发展资金项目（桂科ZY23055047）; 广西茶叶试验站建设专项（桂TS202106）; 广西农业科学院创新团队项目（桂农科2021YT040）

Plasma-Activated Sodium Lactate Enhances Secondary Metabolites and Physiological Resistance of Young Tea Plants

ZHANG Yunfan^1,2, ZHOU Fengjue², HU Junming^1,*, SONG Chuankui³, ZHENG Fuhai¹, ZHANG Junhui¹, LI Tingting¹, LI Yuxiang¹

1. Agricultural Resources and Environmental ResearchInstitute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Arable Land Conservation, Nanning 530007, China;
2. Agricultural College, Guangxi University, Nanning 530004, China;
3. School of Tea Science, Anhui Agricultural University, Hefei 230036, China

Received:2025-06-07 Revised:2025-09-04 Online:2026-02-15 Published:2026-02-06

摘要/Abstract

摘要： 抗逆性是植物通过生理生化调节应对逆境胁迫的适应能力。为明确等离子活化物对氧化胁迫下的幼龄茶树次生代谢物及游离氨基酸的调控机制,采用等离子活化乳酸钠（Plasma-activated sodium lactate,PAL）对幼龄茶树进行胁迫处理,设置高浓度PAL（稀释25倍,P₁）、低浓度PAL（稀释100倍,P₂）两个处理,在温室中培养,通过对照试验和靶向代谢组学研究PAL对茶树的生物量、抗氧化、次生代谢物质和游离氨基酸积累的影响。研究结果表明：（1）茶树喷施PAL能提升幼龄茶树次生代谢物的积累,喷施P₁后表儿茶素没食子酸酯（ECG）含量提高了6.93%;喷施P₂后,ECG和表没食子儿茶素（EGC）分别提高了4.68%和4.84%。（2）PAL改变了幼龄茶树游离氨基酸积累量,显著提高瓜氨酸（Cit）的含量;天冬酰胺（Asn）、组氨酸（His）、苯丙氨酸（Phe）、精氨酸（Arg）、γ-氨基丁酸（GABA）、茶氨酸（Theanine）参与了幼龄茶树抗性的调控,促进茶树生长并参与缓解毒性及物质转运相关蛋白质的合成。（3）PAL能显著增加幼龄茶树的生物量,便于茶树获取更多资源和能量,以应对外界环境胁迫。外源施用PAL可提升幼龄茶树抗逆生理活性并增强茶树活力,是一种调控茶树次生代谢物抗性的重要途径。

关键词: 等离子活化乳酸钠, 幼龄茶树, 次生代谢物, 生理抗性

Abstract: Plant stress resistance refers to the adaptive capacity of plants to cope with environmental stress through physiological and biochemical regulation. Young tea plants have relatively weak physiological resistance. This study aimed to clarify the regulatory mechanism of plasma-activated substances on secondary metabolites and free amino acids in young tea plants under oxidative stress. Plasma-activated sodium lactate (PAL) was used to treat young tea plants under stress conditions. Two dilution gradients were set: a high concentration of 25 times and a low concentration of 100 times. Through control experiments and targeted metabolomics in a greenhouse, the study investigated the effects of plasma-activated sodium lactate on biomass, antioxidant capacity, secondary metabolites, and free amino acid accumulation in tea plants. The results show that: (1) Both concentrations of PAL significantly increased the accumulation of secondary metabolites in young tea plants. Epicatechingallate (ECG) increased by 4.68% at the low concentration and 6.93% at the high concentration, while epigallocatechin (EGC) increased by 4.84% at the low concentration. (2) Plasma sodium lactate altered the accumulation of free amino acids in young tea plants, significantly increasing the contents of citrulline (Cit), asparagine (Asn), histidine (His), phenylalanine (Phe), arginine (Arg), γ-aminobutyric acid (GABA) and theanine (Theanine) in young tea plants, promoting tea plant growth and participating in the synthesis of proteins related to toxicity alleviation and substance transports. (3) Plasma-treated sodium lactate significantly increased the biomass of young tea plants, enabling them to access more resources and energy to cope with external environmental stresses. Exogenous application of plasma-treated sodium lactate influences the stress resistance physiology of young tea plants and enhances their vitality, representing an important method for regulating the stress resistance of secondary metabolites in tea plants.

Key words: plasma-activated sodium lactate, young tea plants, secondary metabolite, physiological resistance

中图分类号:

张云帆, 周凤珏, 胡钧铭, 宋传奎, 郑富海, 张俊辉, 李婷婷, 李宇翔. 等离子活化乳酸钠增强幼龄茶树次生代谢及生理抗性[J]. 茶叶科学, 2026, 46(1): 61-72.

ZHANG Yunfan, ZHOU Fengjue, HU Junming, SONG Chuankui, ZHENG Fuhai, ZHANG Junhui, LI Tingting, LI Yuxiang. Plasma-Activated Sodium Lactate Enhances Secondary Metabolites and Physiological Resistance of Young Tea Plants[J]. Journal of Tea Science, 2026, 46(1): 61-72.

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