普洱茶茶褐素的分级及相关性质初步研究

doi:10.13305/j.cnki.jts.2011.03.006

茶叶科学 ›› 2011, Vol. 31 ›› Issue (3): 187-194.doi: 10.13305/j.cnki.jts.2011.03.006

普洱茶茶褐素的分级及相关性质初步研究

杨新河^1,2, 王丽丽^1,3, 黄建安^1,3, 吴文亮^1,3, 刘仲华^1,3*

1. 湖南农业大学茶学教育部重点实验室, 湖南长沙 410128;
2. 孝感学院生命科学技术学院, 湖北孝感 432000;
3. 国家植物功能成分利用工程技术研究中心, 湖南长沙410128

收稿日期:2010-12-13 修回日期:2011-02-20 出版日期:2011-06-15 发布日期:2019-09-06
通讯作者: Larkin-liu@163.com
作者简介:杨新河（1974— ）,男,湖南益阳人,博士研究生,主要从事植物功能成分化学研究。
基金资助:
科技部支撑计划重大项目(2007BAD58B00-03),云南省政府茶叶专项（2007YNCXB-01-01）

Preliminary Study on Fractions and Correlative Properties of Theabrownin from Pu-erh Tea

YANG Xin-he^1,2, WANG Li-li^1,3, HUANG Jian-an^1,3, WU Wen-liang^1,3, LIU Zhong-hua^1,3*

1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
2. School of Life Science and Technology, Xiaogan College, Xiaogan 432000, China;
3. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingedients, Changsha 410128, China;

Received:2010-12-13 Revised:2011-02-20 Online:2011-06-15 Published:2019-09-06

摘要/Abstract

摘要： 普洱茶水提物依次经氯仿、乙酸乙酯、正丁醇萃取后剩余的水层色素-茶褐素上羟丙基葡聚糖凝胶（sephadex LH-20）柱,以丙酮-水溶液进行洗脱,按照色素条带分别收集得到级分并初步研究它们的理化与光谱性质。结果表明,以40%丙酮-水溶液为洗脱剂,流速为0.05BV/h时,茶褐素的分级效果最好,柱中呈现6条明显的条带,它们的色差参数值、还原力、pH值、常规成分含量、紫外-可见光谱扫描、红外光谱扫描及稳定性等存在差异。该研究进一步表征了普洱茶茶褐素组成与性质的复杂性,同时为深入研究茶褐素的生物活性与化学成分的本质奠定了基础。

关键词: 普洱茶, 茶褐素, 分级, 性质

Abstract: The water extract of pu-erh tea was extracted with chloroform, ethyl acetate, n-butanol in turn. The remaining water pigment-theabrownin obtained was installed in hydroxypropyl glucan gel (sephadex LH-20) column with acetone-aqueous solution for elution and the fractions of theabrownin were collected respectively and their physico-chemical and spectral properties were studied preliminarily. The results indicated that the 40% acetone-aqueous solution was the optimum eluent and flow was 0.05BV/h and classification of theabrownin was best in presenting 6 obvious strips in column, with difference of their chromatism parameter values, total reducing power, pH value, conventional component, UV and IR spectroscopy scanning, and stability etc. The study further demonstrated complexity of compositions and properties of theabrownin. At the same time, this investigation laid a foundation for the further research of bioactivities and chemical essence of theabrownin.

Key words: pu-erh tea, theabrownin, fraction, property

中图分类号:

TS272.5⁺6

杨新河, 王丽丽, 黄建安, 吴文亮, 刘仲华. 普洱茶茶褐素的分级及相关性质初步研究[J]. 茶叶科学, 2011, 31(3): 187-194. doi: 10.13305/j.cnki.jts.2011.03.006.

YANG Xin-he, WANG Li-li, HUANG Jian-an, WU Wen-liang, LIU Zhong-hua. Preliminary Study on Fractions and Correlative Properties of Theabrownin from Pu-erh Tea[J]. Journal of Tea Science, 2011, 31(3): 187-194. doi: 10.13305/j.cnki.jts.2011.03.006.

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普洱茶茶褐素的分级及相关性质初步研究

Preliminary Study on Fractions and Correlative Properties of Theabrownin from Pu-erh Tea

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