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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (4): 242-248.doi: 10.13305/j.cnki.jts.2005.04.002

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Phylogenetic Relationship of Tea Tussork(Euproctis pseudoconspersa) Nucleopolyhedrovirus and Other Baculoviruses Based on P24、Rr1 and Lef1 Genes

Nie Ting-ting1, XIAO Qiang2, YIN Kun-shan2, XING Li-Ping1, ZHANG Chuan-Xi1*   

  1. 1.Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Online:2005-12-25 Published:2019-09-16

Abstract: An EcoRI-XhoI 5942 bp DNA fragment from the Euproctis pseudoconspersa nucleopolyhedrovirus (EupsNPV) genome was cloned and its sequence was determined. Five Open Reading Frames (ORFs) encoding P24, Rr1, 38.7 K, Lef1 and Ep-ld124 were identified in the fragment. Comparative and phylogenetic analysis of the 5 genes with their homologues from other baculoviruses revealed that EupsNPV was a nucleopolyhedrovirus distinct from other known baculoviruses. The result also showed that EupsNPV was one of the NPV II members, and was most closely related to Ectropis obliqua SNPV (EcobSNPV). The sequence data reported in this paper have been assigned the EMBL accession number AJ920288.

Key words: Euproctis pseudoconspersa Strand, Nucleopolyhedrovirus, P24, Lef1, Rr1, Phylogenetic tree

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