Analysis of the Chloroplast Proteome Difference of ‘Baiye 1’ [Camellia sinensis (L.) O Kuntze] during Periodic Albinism

李勤1; 2; 3，程晓梅1，李永迪1，杨培迪4，黄建安1; 3*，刘仲华1; 2; 3*

doi:10.13305/j.cnki.jts.2019.03.010

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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (3) : 325-334. DOI: 10.13305/j.cnki.jts.2019.03.010

Analysis of the Chloroplast Proteome Difference of ‘Baiye 1’ [Camellia sinensis (L.) O Kuntze] during Periodic Albinism

LI Qin^1,2,3, CHENG Xiaomei¹, LI Yongdi¹, YANG Peidi⁴, HUANG Jian'an^1,3*, LIU Zhonghua^1,2,3*

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Abstract

‘Baiye 1’ is a kind of temperature sensitive tea cultivar. The change of chloroplast is the key factor for the periodic albinism of ‘Baiye 1’. To understand the mechanism of periodic albinism of ‘Baiye 1’, two dimensional electrophoresis (2-DE) and mass spectrometry was adopted to separate and identify the chloroplast proteins, which were significantly changed during the three developmental periods. The results show that 726, 748 and 718 protein spots were separated at the pre-albinistic, albinistic and regreen stages, respectively. The expression levels of 59 protein spots varied markedly during the three development stages. A total of 22 protein spots were successfully identified by MS, which were involved in photosynthesis, stress response, metabolism of nucleic acid, substance metabolism and unknown function. Photosynthetic proteins were the most affected proteins, which account for 31.82% in the significantly changed proteins. These results indicate that these physiological processes might play crucial roles in the periodic albinism. The gene expression profiles of the differentially expressed proteins were also verified by real-time PCR analysis. The results show that the expressions of genes and proteins were not consistent, which might be related to the protein processing and post-modification. These results provide a theoretical basis for understanding the molecular mechanism of periodic albinism in ‘Baiye 1’.

Key words

2-DE / Baiye 1 / chloroplast proteome / periodic albinism

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LI Qin, CHENG Xiaomei, LI Yongdi, YANG Peid, HUANG Jian'an, LIU Zhonghua. Analysis of the Chloroplast Proteome Difference of ‘Baiye 1’ [Camellia sinensis (L.) O Kuntze] during Periodic Albinism[J]. Journal of Tea Science. 2019, 39(3): 325-334 https://doi.org/10.13305/j.cnki.jts.2019.03.010

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