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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (3): 325-334.doi: 10.13305/j.cnki.jts.2019.03.010

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Analysis of the Chloroplast Proteome Difference of ‘Baiye 1’ [Camellia sinensis (L.) O Kuntze] during Periodic Albinism

LI Qin1,2,3, CHENG Xiaomei1, LI Yongdi1, YANG Peidi4, HUANG Jian'an1,3*, LIU Zhonghua1,2,3*   

  1. 1. Tea Key Lab of the Ministry of National Teaching of Hunan Agricultural University, Changsha 410128, China; 2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China; 3. Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China; 4. Tea Research Institute, Hunan Academy of Agriculture Sciences, Changsha 410128, China
  • Received:2018-11-05 Revised:2019-01-01 Online:2019-06-15 Published:2019-06-15

Abstract: ‘Baiye 1’ is a kind of temperature sensitive tea cultivar. The change of chloroplast is the key factor for the periodic albinism of ‘Baiye 1’. To understand the mechanism of periodic albinism of ‘Baiye 1’, two dimensional electrophoresis (2-DE) and mass spectrometry was adopted to separate and identify the chloroplast proteins, which were significantly changed during the three developmental periods. The results show that 726, 748 and 718 protein spots were separated at the pre-albinistic, albinistic and regreen stages, respectively. The expression levels of 59 protein spots varied markedly during the three development stages. A total of 22 protein spots were successfully identified by MS, which were involved in photosynthesis, stress response, metabolism of nucleic acid, substance metabolism and unknown function. Photosynthetic proteins were the most affected proteins, which account for 31.82% in the significantly changed proteins. These results indicate that these physiological processes might play crucial roles in the periodic albinism. The gene expression profiles of the differentially expressed proteins were also verified by real-time PCR analysis. The results show that the expressions of genes and proteins were not consistent, which might be related to the protein processing and post-modification. These results provide a theoretical basis for understanding the molecular mechanism of periodic albinism in ‘Baiye 1’.

Key words: Baiye 1, periodic albinism, chloroplast proteome, 2-DE

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