To in vitro and in vivo evaluate and compare the antioxidant and anti-aging activities of white tea with different sources of raw materials, Baihaoyinzhen (YZ), Baimudan (MD), Shoumei (SM) were prepared and extracted to measure the main efficacy components. In vitro antioxidant assay, the antioxidantβactivitiesβwereβ also tested. For in vivo anti-aging test, the mice were subcutaneously injected with D-galactose and separately orally administered white tea extracts for aging model analysis. The contents of main efficacy components in YZ extract were higher than those in MD and SM extracts. The contents of tea polyphenols and flavonoids in SM extract were higher than those in MD extract. In vitro, YZ extract had better antioxidant capacity, and there was no obvious difference between SM and MD extract. In vivo, the degree of liver lipid peroxidation in YZH and YZL mice were significantly (P<0.05) decreased. The antioxidant enzyme activities were significantly (P<0.05) increased in serum of SMH and SML mice. But it did not show a dose-dependent manner. The 3 kinds of white tea extracts could up-regulate the expression levels of antioxidant enzymes and anti-aging genes to improve the oxidative stress state and it showed a dose-dependent manner. And there was no hepatotoxicity in these group mice.
[1] 中华人民共和国国家质量监督检验检疫总局. GB/T 30766—2014 茶叶分类[S]. 北京: 中国标准出版社, 2014-10-27.
[2] 叶乃兴. 白茶、白叶茶与白毛茶[J]. 福建茶叶, 2010, 32(10): 44-46.
[3] 邓静, 王远兴, 丁建. 白茶与安吉白茶的研究进展[J]. 食品工业科技, 2013(4): 368-371.
[4] 佚名. 白茶保健养生功效研究成果首次权威发布[J]. 茶世界, 2012(6): 25-26.
[5] Luo L N, Liu L Y, Sun Y, et al.Variations of Main Biochemical Components in White Tea during the Continuous and Warmed Withering[J]. Tea Science & Technology, 2014(2): 8-11.
[6] Azizi S, Shahri M M, Rahman H S, et al.Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line[J]. International Journal of Nanomedicine, 2017, 12: 8841.
[7] Santana-Rios G, Orner G A, Amantana A, et al.Potent antimutagenic activity of white tea in comparison with green tea in the Salmonella, assay[J]. Mutation Research, 2001, 495(1/2): 61.
[8] 王刚, 赵欣. 两种白茶的抗突变和体外抗癌效果[J]. 食品科学, 2009, 30(11): 243-245.
[9] 郑丽, 侯彩云, 任发政. 几种茶叶提取物预防高脂饮食小鼠肥胖的研究[J/OL]. 食品科学: 1-8. [2017.6.11]. http://kns. cnki.net/kcms/detail/11.2206.ts.20170519.1049.140.html.
[10] 吕海鹏, 张悦, 陈兴华, 等. 不同花色种类白茶的抗氧化活性及其主要品质化学成分分析[J]. 食品科学, 2016, 37(20): 42-50.
[11] 中华人民共和国卫生部. 保健食品检验与评价技术规范(2003年版)[S]. 北京: 卫生部卫生法制与监督司编印, 2003: 115-117.
[12] 中华人民共和国国家质量监督检验检疫总局. GB/T 8313—2008. 茶叶中茶多酚和儿茶素含量的检测方法[S]. 北京: 中国标准出版社, 2008-05-04.
[13] 宗春燕, 苏学军. 三氯化铝比色法测定稻壳中总黄酮含量的方法研究[J]. 化学工程师, 2017(10): 66-69.
[14] 中华人民共和国国家质量监督检验检疫总局. GB/T 8312—2013. 茶咖啡碱测定[S]. 北京: 中国标准出版社, 2013-12-31.
[15] Ramadan MF, Kroh LW, Morsel JT.Radical scavenging activity of black cumin (Nigella sativa L.) coriander (Coriandrum sativum L.) and niger (Guizotia abyssinca Cass.) crude seed oils and oil fractions[J]. Journal of Agricultural & Food Chemisty, 2003, 51: 6961-6969.
[16] 王蝶. 茶叶对肥胖大鼠的减肥作用及机制研究[D]. 长沙: 湖南农业大学, 2012.
[17] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-\Delta\Delta C_{T}}$ method[J]. Methods, 2012, 25(4): 402-408.
[18] Sang S, Lambert J D, Ho C T, et al.The chemistry and biotransformation of tea constituents[J]. Pharmacological Research, 2011, 64(2): 87-99.
[19] Tao L, Forester S C, Lambert J D.The role of the mitochondrial oxidative stress in the cytotoxic effects of the green tea catechin, (-)-epigallocatechin-3-gallate, in oral cells[J]. Molecular Nutrition & Food Research, 2014, 58(4): 665-676.
[20] Basu A, Betts N M, Mulugeta A, et al.Green tea supplementation increases glutathione and plasma antioxidant capacity in adults with the metabolic syndrome[J]. Nutrition Research, 2013, 33(3): 180-187.
[21] Zhao Y, Chen P, Lin L, et al.Tentative identification, quantitation, and principal component analysis of green pu-erh, green, and white teas using UPLC/DAD/MS[J]. Food Chemistry, 2011, 126(3): 1269.
[22] Oda Y, Hidaka M, Suzuki A.Caffeine has a synergistic anticancer effect with cisplatin via inhibiting Fanconi anemia group D2 protein monoubiquitination in hepatocellular carcinoma cells[J]. Biological & Pharmaceutical Bulletin, 2017, 40(11): 2005-2009.
[23] 陈燕, 樊柏林, 田洁, 等. 虾青素人参茶叶对老龄小鼠的抗氧化作用[J]. 公共卫生与预防医学, 2016, 27(2): 34-37.
[24] 吴克芬, 胡予. D-半乳糖致衰老动物模型的建立及评价[J]. 中华老年多器官疾病杂志, 2012, 11(1): 74-78.
[25] Su J J, Wang X Q, Song W J, et al.Reducing oxidative stress and hepatoprotective effect of the water extracts from Pu-erh tea on rats fed with high-fat diet[J]. Food Science & Human Wellness, 2016, 5(4): 199-206.
[26] Sun S.Free radical scavenging abilities in vitro and antioxidant activities in vivo of black tea and its main polyphenols[J]. Journal of Medicinal Plant Research, 2012, 6(1): 114-121.
[27] Peng X, Kong B, Yu H, et al.Protective effect of whey protein hydrolysates against oxidative stress in d-galactose-induced ageing rats[J]. International Dairy Journal, 2014, 34(1): 80-85.
[28] Wang L, Yao Y, He R, et al.Methane ameliorates spinal cord ischemia-reperfusion injury in rats: antioxidant, anti-inflammatory and anti-apoptotic activity mediated by Nrf2 activation[J]. Free Radical Biology & Medicine, 2017, 103: 69-86.
[29] 郭煜晖, 胡大军, 陈静, 等. 衰老相关基因Klotho的研究进展[J]. 中国老年学, 2017, 37(3): 759-761.
[30] 万艳, 常剑波, 白艳霞, 等. 血清学指标在非酒精性脂肪性肝病诊断中的意义[J]. 临床肝胆病杂志, 2017, 33(5): 963-968.
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