Small GTPase binding proteins are a kind of important signal transduction proteins, which are involved in various life activities of plants. However, few relative studies were reported in tea plants (Camellia sinensis). Here, a small GTPase binding protein named CsRAC5 was cloned by using a cDNA template from tea cultivar ‘Longjingchangye’. The results showed that the length of its open reading frame (ORF) is 597βbp, encoding 198 amino acids. It has a conserved Rho domain which belongs to ROP family. Multiple alignment of CsRAC5 with homologue genes in other plant species showed that their identity could reach 95.96%. CsRAC5 is a hydrophilic protein with the theoretical relative weight of 21.79βkDa. The subcellular assay showed that CsRAC5 was localized in the nuclear and membrane. In addition, the results of RT-PCR analysis showed that the highest expression level of CsRAC5 was in leaves but the lowest in pollen. The expression level of CsRAC5 was decreased under cold stress.
YE Xiaoli
,
PAN Junting
,
ZHU Jiaojiao
,
SHU Zaifa
,
CUI Chuanlei
,
XING Anqi
,
NONG Shouhua
,
ZHU Xujun
,
FANG Wanping
,
WANG Yuhua
. Cloning and Expression Analysis of Small GTPase (CsRAC5) under Cold Stress in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2018
, 38(2)
: 146
-154
.
DOI: 10.13305/j.cnki.jts.2018.02.005
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