To explore the genetic basis for important traits, the full-length transcriptome of the ‘Yuncha 1’ (Camellia assamica) was sequenced by using PacBio Platform. A total of 213β389 polished consensus were generated, 223β120 coding sequences were predicted and annotated, and 195β062 SSR loci were found. According to NR databases, 170β264 homologous sequences were mapped to 980 species, 103β124 unigenes were further annotated and grouped into 26 functional categories in KOG databases, 65β524 unigenes were annotated against GO database and divided into cellular component, molecular function and biological process categories with a total of 55 functional groups. KEGG pathway analysis showed that 105β972 unigenes could be broadly classified into 216 metabolism pathways according to their function, and some of them were involved in quality, bioactive substances, and resistance gene, etc. It is also predicted that there were 5β785 transcription factors belonging to 60 transcription factor families. The experimental results will give important data for development of SSRs of specific traits, genetic analysis and studies involved in quality formation and resistence mechanism in tea cultivar ‘Yuncha 1’.
ZHU Xingzheng
,
XIA Lifei
,
CHEN Linbo
,
SUN Yunnan
,
TIAN Yiping
,
SONG Weixi
,
JIANG Huibin
. Full-length Transcriptome Analysis of Protected Cultivation ‘Yuncha 1’ (Camellia sinensis Var assamica)[J]. Journal of Tea Science, 2018
, 38(2)
: 193
-201
.
DOI: 10.13305/j.cnki.jts.2018.02.010
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