The full cDNA length of NRT2.5 gene was obtained via rapid amplification of cDNA ends (RACE) from tea plant [Camellia sinessis (L.)] leaves. The cDNA sequence of this gene was 2 457 bp and opening reading frame (ORF) was 1 362 bp, encoding for 454 amino acids. The putative protein of this gene had an isoelectric point of 9.63 and a calculated molecular weight of 48.7 kD. Bioinformatics analysis showed that NRT2.5 of tea plant was highly homologous to the genes such as TcNRT2.5 and AtNRT2.5, and its encoded protein had the common structural characteristics of NRT family. Fluorescence quantitative real-time PCR analysis showed that NRT2.5 gene were detected in all the tested tissues of tea plant but was mainly detected in the mature leaves and roots. The expression level in different nitrogen concentration showed that the expression of NRT2.5 gene in low N concentration was significant higher than that in high concentration.
FENG Suhua
,
WANG Liyuan
,
CHEN Changsong
,
LIN Zhenghe
,
CHENG Hao
,
WEI Kang
,
ZHANG Chengcai
. Cloning and Expressing Analysis of a Nitrogen Transporter 2.5 Gene from Tea Plant[Camellia sinensis (L.)][J]. Journal of Tea Science, 2014
, 34(4)
: 364
-370
.
DOI: 10.13305/j.cnki.jts.2014.04.007
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