To identify male parent of Xiangbolv 2 and to provide a reference for the choice of DNA marker in the study of relationship analysis, SSR, SRAP and ISSR were used to identify the male parent of Xiangbolv 2 among 5 possible tea varieties. Percentage of polymorphic bands (PPB), polymorphic information content (PIC), diversity index (DI), resolving power (Rp), marker index (MI) and other indicators were used to compare the marker efficiencies of SSR, SRAP and ISSR. Totally 83 (2.86 per primer) alleles were identified by 29 pairs of SSR primers. The average PPB, PIC, DI, Rp, MI were 77.01%, 0.55, 0.29, 1.31, 0.95 respectively. Meanwhile, 139 (8.18 per primer) alleles were amplified by 17 pairs of SRAP, with the average PPB, PIC, DI, Rp, MI of 71.70%, 0.84, 0.15, 3.65, 3.59 respectively. 90 (9.00 per primer) alleles were detected by 10 ISSR primer, with the average PPB, PIC, DI, Rp, MI of 69.76%, 0.85, 0.15, 4.21, 4.71 respectively. MI, Rp and PIC showed the same trend among SSR, SRAP and ISSR, with the highest value of ISSR, followed by SRAP and SSR, the PPB and DI showed the opposite trend. These results indicated that ISSR and SRAP markers had high marker efficiency, and SSR markers had extensive polymorphism. Similarity coefficients among 7 accessions were different by different 3 DNA markers, but Xiangbolv 2, Xiangbolv and Fuding Dabaicha were gathered together irrespective of DNA markers, indicating that Xiangbolv might be the male parent of Xiangbolv 2.
LIU Zhen
,
ZHAO Yang
,
YANG Peidi
,
CHENG Yang
,
NING Jing
,
YANG Yang
. Comparison of Parents Identification for Tea Variety Based on SSR, SRAP and ISSR Markers[J]. Journal of Tea Science, 2014
, 34(6)
: 617
-624
.
DOI: 10.13305/j.cnki.jts.2014.06.024
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