Differences of gene expression between young and mature leaves of tea plant (Camellia sinensis var. assamica, cultivar Zijuan) were studied by cDNA-AFLP. The results showed that 59 Transcript-Derived Fragments (TDFs) were obtained, including 26 up-regulated TDFs from young leaves, and 33 up-regulated TDFs from mature leaves. According to blasting results, these TDFs were included signal transduction genes, transcription factors, primary metabolism genes, putative protein and unknown protein and no significant homology found. RT-PCR was used to analyze the expression of ZM4, ZM7, ZM12, showed that expression of these ZM4, ZM7, ZM12 increased obviously in mature leaves. This study established a basis for further understanding the purple mechanism of tea leaves in cultivar “Zijuan” and related gene cloning.
CHEN Lin-bo
,
XIA Li-fei
,
SUN Yun-nan
,
LIANG Ming-zhi
,
ZHANG Zheng-zhu
,
LI Ye-yun
,
WAN Xiao-chun
. Analysis of Differential Gene Expression on Specific Tea Cultivar “Zijuan” for Leaf Color Changing[J]. Journal of Tea Science, 2012
, 32(1)
: 59
-65
.
DOI: 10.13305/j.cnki.jts.2012.01.013
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