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Study on Process of Anthocyanins Extraction from ‘Zijuan’ Tea by Response Surface Analysis

  • FEI Xu-yuan ,
  • LIN Zhi ,
  • LIANG Ming-zhi ,
  • WANG Li-bo ,
  • CHEN Ji-wei ,
  • LV Hai-peng ,
  • TAN Jun-feng ,
  • GUO Li
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  • 1. Tea Research Insititute, Chinese Academy of Agricultual Sciences, National Engineering Technology Research Center for Tea Industry; Key Laboratory of Processing and Quality Control of Tea and Beverage Plants, Ministry of Agriculture, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100089, China;
    3. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China

Received date: 2012-01-12

  Revised date: 2012-02-08

  Online published: 2019-09-05

Abstract

Process extraction of anthocyanins from Zijuan tea was optimized by response surface methodology (RSM). Selected test factors and levels on the basis of the single-factor test, analysis of the significance of each factor and their interaction by using three factors and three levels of response surface analysis according to central composite (Box-Behnken) experimental design principles. The results showed that the optimum conditions for the extraction Zijuan tea anthocyanin are reaction temperature 29℃, reaction time 132min, the material-liquid ratio 1︰26 in acidic methanol, under these conditions the concentration of anthocyanins in the extract can be achieved 5.94mg/mL.

Cite this article

FEI Xu-yuan , LIN Zhi , LIANG Ming-zhi , WANG Li-bo , CHEN Ji-wei , LV Hai-peng , TAN Jun-feng , GUO Li . Study on Process of Anthocyanins Extraction from ‘Zijuan’ Tea by Response Surface Analysis[J]. Journal of Tea Science, 2012 , 32(3) : 197 -202 . DOI: 10.13305/j.cnki.jts.2012.03.002

References

[1] 包云秀, 夏丽飞, 李友勇, 等. 茶树新品种紫娟[J]. 园艺学报, 2008, 35(6): 934.
[2] 季鹏章, 梁名志, 宋维希, 等. 茶树珍稀品种紫娟的叶片色素含量与叶色变化的关系研究[J]. 西南农业学报, 2010, 23(6): 1860-1862.
[3] A. Degenhardt.Separation and Purification of Anthocyanins by High-Speed Countercurrent Chromatography and Screening for Antioxidant Activity[J]. J Agric Food Chem, 2000, 48(2): 338-343.
[4] 宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2007.
[5] Tsuda T, Horio F, Kitoh J, et al. Protective effects of dietary cyanidin 3-O-beta-D-glucoside on liver ischemia-reperfusion injury in rats[J]. Arch Biochem Biophys, 1999, 368(2): 361-366.
[6] Narayan M S, Naidu K A, Ravishankar G A, et al. Antioxidant effect of anthocyanin on enzymatic and non-enzymatic lipid peroxidation[J]. Prostaglandins Leukot Essent Fatty Acids, 1999, 60(1): 1-4.
[7] Pacheco-Palencia L A, Talcott S T. Chemical stability of acai fruit (Euterpe oleracea Mart) anthocyanins as influenced by naturally occurring and externally added polyphenolic cofactors in model systems[J]. Food Chemistry, 2010, 118(1): 17-25.
[8] Cignarella A, Nastasi M, Cavalli F, et al. Novel lipid-lowering properties of Yaccinium myrtillus L. leaves,a traditional antidiabetic treatment, in several models of rat dyslipidaemia: a comparison with ciprofibrate[J]. Thromb Res, 1996, 84(5): 311-322.
[9] 杨玲, 刘利军, 赵永莉. 不同提取剂对紫罗兰马铃薯花青素提取含量的影响[J]. 石油化工应用, 2008, 27(5): 6-8.
[10] 肖丽霞, 汪芬, 张超, 等. 提取剂对紫玉米花青素提取量的影响[J]. 农产品加工, 2009(6): 38-41.
[11] 罗正飞, 梁名志, 夏丽飞, 等. “紫娟”茶花青素提取工艺研究[J]. 福建茶叶, 2011(3): 22-24.
[12] 陈健, 孙爱东, 高雪娟, 等. 响应面分析法优化超声波提取槟榔原花青素工艺[J]. 食品科学, 2011, 32(4): 82-86.
[13] 黄意欢. 茶学试验技术[M]. 北京: 中国农业出版社, 1995.
[14] 董发明, 白喜婷. 响应面法优化超声提取杜仲雄花中黄酮类化合物的工艺参数[J]. 食品科学, 2008, 29(8): 227-231.
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