The aim of this study was to show the effects of long-term fertilization on the activities of protease, urease, invertase, catalase and phosphatase of the tea garden soil in red-yellow soil area. It consisted of six treatments: no fertilization, the whole rate of chemical fertilizer, half rate of chemical fertilizer + half rate of organic fertilizer, the whole rate of organic fertilizer, the whole rate of chemical fertilizer + leguminous green manure and half rate of chemical fertilizer + half rate of organic fertilizer + leguminous green manure. Results indicated that: The activities of protease, urease and phosphatase in soil were enhanced by all those treatments, especially by half rate of chemical fertilizer + half rate of organic fertilizer + leguminous green manure treatment, the protease activity was increased 2.82 times, urease by 7.73 times and phosphatase by 0.33 times compared to those under no fertilization treatment, while the activities of those enzymes in the whole rate of chemical fertilizer treatment were less than those in half rate of chemical fertilizer + half rate of organic fertilizer + leguminous green manure treatment. In addition, organic matter, soil total N and P, hydrolysable N, available P and K, soil water content, cation exchange capacity, plant height and tea yield were highly positively correlated with individual enzymes such as protease, urease and phosphatase rather than catalase activity, respectively. It is concluded that the protease, urease and phosphatase showed the most sensitive to the treatments, suggesting that those enzymes should be used as sensitive indicators of variation in soil quality and the growth vigor of tea plant induced by different fertilization treatment. Overall, a faster nitrogen and phosphorus turn-over is found under half rate of chemical fertilizer + half rate of organic fertilizer + leguminous green manure treatment, which is beneficial to increase the supply of these nutrients to tea plant.
WANG Li-min
,
LI Wei-hua
,
FAN Ping
,
QIU Xiao-xuan
,
HUANG Dong-feng
,
LIN Xin-jian
. Variation in Soil Enzyme Activities under Long-term Fertilization of Tea Garden in Red-yellow Soil Area[J]. Journal of Tea Science, 2012
, 32(4)
: 347
-352
.
DOI: 10.13305/j.cnki.jts.2012.04.012
[1] 李娟, 赵秉强, 李秀英, 等. 长期有机无机肥料配施对土壤微生物学特性及土壤肥力的影响[J]. 中国农业科学, 2008, 41(1): 144-152.
[2] Khorsandi N, Nourbakhsh F.Effect of amendment of manure and corn residues on soil N mineralization and enzyme activity[J]. Agronomy for Sustainable Development, 2007, 27(2): 139-143.
[3] Elfstrand S, Hedlund K, Mårtensson A.Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring[J]. Applied Soil Ecology, 2007, 35(3): 610-621.
[4] Harris J A.Measurements of the soil microbial community for estimating the success of restoration[J]. European Journal of Soil Science, 2003, 54(4): 801-808.
[5] Caldwell B A.Enzyme activities as a component of soil biodiversity: a review[J]. Pedobiologia, 2005, 49(6): 637-644.
[6] 姚槐应, 何振梁, 陈国潮, 等. 红壤微生物量在土壤-黑麦草系统中的肥力意义[J]. 应用生态学报, 1999, 10(6): 725-728.
[7] Roldán A, Salinas-García J R, Alguacil M M, et al. Soil enzyme activities suggest advantages of conservation tillage practices in sorghum cultivation under subtropical conditions[J]. Geoderma, 2005, 129(3/4): 178-185.
[8] Ushio M, Kitayama K, Balser T C.Tree species effects on soil enzyme activities through effects on soil physicochemical and microbial properties in a tropical montane forest on Mt. Kinabalu, Borneo[J]. Pedobiologia, 2010, 53(4): 227-233.
[9] Kim H, Kang H.The impacts of excessive nitrogen additions on enzyme activities and nutrient leaching in two contrasting forest soils[J]. The Journal of Microbiology, 2011, 49(3): 369-375.
[10] Iyyemperumal K, Shi W.Soil enzyme activities in two forage systems following application of different rates of swine lagoon effluent or ammonium nitrate[J]. Applied Soil Ecology, 2008, 38(2): 128-136.
[11] 王芸, 韩宾, 史忠强, 等. 保护性耕作对土壤微生物特性及酶活性的影响[J]. 水土保持学报, 2006, 20(4): 120-122, 142.
[12] 中华人民共和国林业部科技司. 林业标准汇编[G]. 北京: 中国林业出版社, 1991: 96-293.
[13] 杨兰芳, 曾巧, 李海波, 等. 紫外分光光度法测定土壤过氧化氢酶活性[J]. 土壤通报, 2011, 42(1): 207-210.
[14] 姚槐应, 黄昌勇. 土壤微生物生态学及其实验技术[M]. 北京: 科学出版社, 2006: 186-191.
[15] 关松荫, 沈桂琴, 孟昭鹏, 等. 我国主要土壤剖面酶活性状况[J]. 土壤学报, 1984, 21(4): 368-381.
[16] Nayak D R, Babu Y J, Adhya T K.Long-term application of compost influences microbial biomass and enzyme activities in a tropical Aeric Endoaquept planted to rice under flooded condition[J]. Soil Biology and Biochemistry, 2007, 39(8): 1897-1906.
[17] Chang E H, Chung R S, Tsai Y H.Effect of different application rates of organic fertilizer on soil enzyme activity and microbial population[J]. Soil Science and Plant Nutrition, 2007, 53(2): 132-140.
[18] Stark C H, Condron L M, O’Callaghan M, et al. Differences in soil enzyme activities, microbial community structure and short-term nitrogen mineralization resulting from farm management history and organic matter amendments[J]. Soil Biology and Biochemistry, 2008, 40(6): 1352-1363.
[19] Shen W, Lin X, Shi W, et al. Higher rates of nitrogen fertilization decrease soil enzyme activities, microbial functional diversity and nitrification capacity in a Chinese polytunnel greenhouse vegetable land[J]. Plant and Soil, 2010, 337(1/2): 137-150.
[20] Gunapala N, Scow K M.Dynamics of soil microbial biomass and activity in conventional and organic farming systems[J]. Soil Biology and Biochemistry, 1998, 30(6): 805-816.
[21] 张科, 袁玲, 施娴, 等. 不同植烟模式对烤烟产质量、土壤养分和酶活性的影响[J]. 植物营养与肥料学报, 2010, 16(1): 124-128.
[22] Busto M D, Perez-Mateos M.Extraction of humic-β-glucosidase fractions from soil[J]. Biology and Fertility of Soils, 1995, 20(1): 77-82.
[23] Rao M A, Violante A, Gianfreda L.Interaction of acid phosphatase with clays, organic molecules and organo-mineral complexes: kinetics and stability[J]. Soil Biology and Biochemistry, 2000, 32(7): 1007-1014.
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