SNPs were mined from tea-derived EST sequences. Primers were designed to evaluate the predicted SNPs in genomic DNA from tea plant. The PCR products were sequenced from both directions to confirm the valid SNPs. Meanwhile, the dCAPS primers were designed based on the valid SNPs and the genomic DNA was used for amplification. The amplicons were digested by restrict enzymes to convert SNPs into dCAPS markers. Furthermore, we also detected the separation of the new markers in a F1 population. The results showed that seventeen SNPs (54.8%) were confirmed in 11 amplycons, eight SNPs were successfully converted to dCAPS markers (72.7%), two of them which have polymorphisms in parents exhibited a separate ratio of 1︰1 in the F1 population. The new markers would be useful in tea genetic research. It is also believed that dCAPS technology will promote the use of SNP technology in genetics and breeding research in tea plant.
Key words：
tea; EST; SNP; dCAPS
ZHANG Cheng-cai
,
WANG Li-yuan
,
WEI Kang
,
CHENG Hao
,
BAO Yun-xiu
,
LIU Ben-ying
,
WANG Yun-gang
. Study of SNP and Relative dCAPS Markers in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2012
, 32(6)
: 517
-522
.
DOI: 10.13305/j.cnki.jts.2012.06.013
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