The polyphenol oxidase (PPO, GenBank accession No. DQ812086) gene was amplified by PCR. Bioinformatics analysis indicated that the cds of PPO DNA sequence is 1800bp, which encoded a protein of 599 amino acid residues (molecular formula: C3008H4677N813O900S18) and predicted that it had two Cu-band functional domains with no transmembrane. The phylogeny analysis showed that it has a most close phylogenetic relationship with Camellia nitidissima. The PPO was cloned into pET28a vector to construct recombination prokaryotic expression vector pET-ppo. After transformed to E1coli BL21 and induced by isopropyl-β-D-thiogalactopyranoside(IPTG), recombinant protein about 71kD was expressed in pET28a system and separated by SDS-PAGE electrophoresis.
WANG Nai-dong
,
ZHANG Li-xia
,
XIANG Qin-zeng
,
LI Wei-wei
. Bioinformatic Analysis and Prokaryotic Expression of Polyphenol Oxidase Gene in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2011
, 31(1)
: 33
-39
.
DOI: 10.13305/j.cnki.jts.2011.01.006
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