Two-dimensional electrophoresis(2-DE) were carried out to study the proteomic change in of tea leaves in responsive to polyethylene glycol(PEG) stress, and expression profiles of 18 different proteins were analyzed under PEG stress. Sixteen different proteins were identified by MS/MS using 4700 Proteomics Analyzer(Applied Biosystems, USA),and 14 proteins were identified and represented 10 kinds of proteins. In these proteins, 5 were the same protein—ribulose 1, 5-bisphosphate carboxylase/oxygenase large subunit(RubisoLSU), other 9 proteins were response proteins to PEG stress including photosystem II 23 kDa polypeptide,chloroplast chaperonin 21, triose phosphate isomerase(TPI), ATP synthase subunit, S-adenosylmethionine synthetase 1(SAMS1), centroradialis(CEN), nascent polypeptide associated complex alpha chain(α-NAC), 20 S proteasome alpha subunit E2(PAE2) and translation initiation factor 5A(eIF5A). These proteins will play an important role in many life activities of plant, such as chloroplast composition, sugar metabolism, energy metabolism, sulfur metabolism, protein metabolism, signal transduction, gene expression regulation and programmed cell death.
GUO Chun-fang
,
SUN Yun
,
LAI Cheng-chun
,
ZHANG Mu-qing
. Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress[J]. Journal of Tea Science, 2009
, 29(2)
: 79
-88
.
DOI: 10.13305/j.cnki.jts.2009.2.001
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