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Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis

  • LIU Ya-jun ,
  • GAO Li-ping ,
  • XIA Tao ,
  • GAO Ke-jun
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  • 1. Key Lab of Tea Biochemistry & Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China;;
    2. School of Biology Science, Anhui Agricultural University, Hefei 230036, China

Received date: 2008-07-18

  Revised date: 2008-11-17

  Online published: 2019-09-06

Abstract

To preserve eminent cell lines with stable metabolism, the cryopreservation of suspension culture cells by vitrification in Camellia sinensis has been preliminarily investigated in this article. It showed that the preculture for four days was best, loading with 60% PVS2 in freezing bath for 20 minutes was optimal. Treating with 100% PVS2 dehydration in freezing bath for 60 minutes was suggested. The cryopreservation of suspension culture cells warmed in 40℃water bath had the highest survival rate. The cells’ survival rate was as high as 76% when these parameters were adopted through the whole trial. The procedure of cryopreservation of suspension culture cells by vitrification in Camellia sinensis was preliminarily established.

Cite this article

LIU Ya-jun , GAO Li-ping , XIA Tao , GAO Ke-jun . Study on Cryopreservation of Suspension Culture Cells by Vitrification in Camellia Sinensis[J]. Journal of Tea Science, 2009 , 29(2) : 120 -126 . DOI: 10.13305/j.cnki.jts.2009.2.006

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