In order to develop an efficient protein extraction method suitable for two-dimensional electrophoresis from tea plant, three methods (TCA/acetone precipitation, improved Tris-HCl extraction and phenol-methanol/ammonium acetate precipitation) for total protein extraction in plant were compared. The result showed that the improved Tris-HCl extraction was the best method suitable for tea leaf protein sample preparation in these three techniques and the extracts were separated by two-dimensional electrophoresis. Consequently, 1117±9.89 protein spots were found and their isoelectric points were found to lie between pI5~7. The relative molecular weight of proteins was between 15.00~95.00kD. The improved Tris-HCl extraction could get the highest resolution of two dimensional electrophoresis and high yield of protein, and it could meet the requirement for the research on tea proteomecis.
LI Qin
,
HUANG Jian-an
,
LIU Shuo-qian
,
LI Juan
,
LIU Zhong-hua
. Protein Extraction Method Suitable for Tea Plant[J]. Journal of Tea Science, 2011
, 31(3)
: 173
-178
.
DOI: 10.13305/j.cnki.jts.2011.03.014
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