In order to produce xylose from tea seed shells, The method of hydrolysis of tea seed shells under preeure was used, and the dilute hydrochloric acid as a catalyst, and the ultrasonic waveas the assisted method. The xylose was monitored by 3,5-dinitrosalicylic acid (DNS) colorimetric method. The hydrolytic factors including pre-extraction time, hydrochloric acid concentration, hydrolysis time were investigated by means of orthogonal test. Results have demonstrated that the importance of various factors were arranged in following order: time of hydrolysis>concentration of acid>particlesize>time of ultrasonic wave pre-extration. The optimum factors production were as follows: 45min, ultra-sonic wave pre-extration, ultrasonic frequency of 50kHz at 25~30℃, hydrochloric acid concentration at 0.6mol/L, particle size of seed shell at 40 mesh hydrolysis time for 3h. Under the above mentioned conditions, the highest productivity of xylose was obtained, the yield was up to 28.44%. This study provided an simple method on the comprehensive utilization of tea seed shells.