In this study, Aspergillus niger H-15 and yeast stain P1,the dominant microorganisms, isolated from Puer tea were used to ferment green tea in liquid. Results showed that the changes of active components during liquid fermentation were different from those of solid-state fermentation. During liquid fermentation, tea polyphenols and caffeine showed a small dynamic change. Content of amino-acid decreased from 0.58mg/mL to 0.14mg/mL, soluble sugar decreased from 13.38mg/mL to 11.34mg/mL, and pH droped from 4.93 to 3.25. Ester catechins were degraded and could not be detected by HPLC after 40h, and the non-galloylated catechins, such as EGC、DL-C、EC and GA increased significantly, their increase rate up to 589.9%、26.5%、139.9% and 219.4% respectively.
LIU Wen-yao
,
CHEN Zhong-zheng
,
LI Bin
,
FANG Xiang
. Studies on the Changes of Components of GreenTea by Mixed Liquid Fermentation[J]. Journal of Tea Science, 2011
, 31(3)
: 225
-229
.
DOI: 10.13305/j.cnki.jts.2011.03.009
[1] 薛水英, 张勇. 普洱茶的主要化学成分及其保健作用的研究[J]. 农业与技术, 2008, 28(5): 71-73.
[2] 方祥, 李斌, 陈栋, 等. 普洱茶功效成分及其品质形成机理研究进展[J]. 食品工业科技, 2008, 29(6): 314-315.
[3] Michiharu Abe, Naohiro Takaoka, Yoshito Idemoto, et al. Characteristic fungi observed in the fermentation process for Puer tea[J]. International Journal of Food Microbiology, 2008, 124(2): 199-203.
[4] 周春红, 黄振兴, 阮文权, 等. 微生物对普洱茶渥堆过程中特定风味成分变化的影响[J]. 食品与发酵工业, 2009, 35(7): 36-39.
[5] 王汉生. 茶叶生物化学实验[M]. 广州: 华南农业大学, 1998: 13-15.
[6] 傅冬和, 刘仲华, 黄建安, 等. 茯砖茶加工过程中主要化学成分的变化[J]. 食品科学, 2008, 29(2): 64-67.
[7] 朱亨政. 柠檬酸发酵[J]. 食品与发酵工业, 1994(6): 69-74.
[8] 梁名志, 孙荣琴. 普洱茶品质化学研究进展[C]. 云南省首届普洱茶国际研讨会论文集编委会. 云南省首届普洱茶国际研讨会论文集, 2004: 121-128.
[9] 张新福, 龚加顺, 周红杰, 等. 云南普洱茶中多酚类物质与品质的关系研究[J]. 食品科学, 2008, 29(4): 230-233.
[10] 吕海鹏, 林智, 谷记平, 等. 普洱茶中的没食子酸研究[J]. 茶叶科学, 2007, 27(2): 104-110.
[11] 张冬英, 邵宛芳, 刘仲华, 等. 普洱茶功能成分单体降糖降脂作用研究[J]. 茶叶科学, 2009, 29(1): 41-46.
[12] 赵远艳, 吕有才, 冯雷. 普洱茶加工过程中儿茶素变化规律的研究[J]. 茶叶通讯, 2008, 35(3): 6-8.
[13] 黄群, 李彦坡, 陈林杰, 等. 冠突散囊菌液态发酵过程中黑茶活性成分变化研究[J]. 食品科学, 2007, 28(12): 232-233.
[14] 王赞苗, 李永刚. 单宁酶对酯型儿茶素作用研究[J]. 中国食品添加剂, 2005(1): 42-44.
[15] 宁井铭, 方世辉, 夏涛, 等. 单宁酶及协同物质对绿茶饮料稳定性的影响[J]. 食品与发酵工业, 2006, 32(6): 69-72.