In the present work, the effect of treatments with EGCG, Zn2+ and EGCG+Zn2+ on inducing apoptosis and content of adenylate nucleotide (ATP, ADP and AMP) in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC. Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 µmol/L EGCG, 80 µmol/L Zn2+ and 80 µmol/L EGCG+80 µmol/L Zn2+ in PC-3 cells. In the determination of adenylate nucleotide, after treated with EGCG, Zn2+ and EGCG+Zn2+, content of total adenylate nucleotide, EC and ATP/ADP value were significantly decreased in PC-3 cells, which demonstrated that EGCG and Zn2+ have a markedly inhibitory effect on energy metabolism of PC-3 cells.
SUN Shi-li
,
LING Cai-jin
,
YU Hai-ning
,
SHEN Sheng-rong
,
MIAO Ai-qing
,
PAN Shun-shun
,
PANG Shi
,
ZHUO Min
,
ZHAO Chao-yi
. Effect of Interactions Between EGCG and Zn2+ on Energy Metabolism of PC-3 Cells[J]. Journal of Tea Science, 2009
, 29(4)
: 251
-256
.
DOI: 10.13305/j.cnki.jts.2009.4.001
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